Summary of Study ST002166
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001378. The data can be accessed directly via it's Project DOI: 10.21228/M8M12K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002166 |
| Study Title | Untargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours - NMR Study. |
| Study Type | Untargeted metabolic profiling |
| Study Summary | Comprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours |
| Institute | Institute of Nuclear Medicine and Allied Sciences |
| Last Name | Rana |
| First Name | Poonam |
| Address | DRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India |
| poonam@inmas.drdo.in | |
| Phone | +911123905319 |
| Submit Date | 2021-11-29 |
| Num Groups | 3 |
| Total Subjects | 21 |
| Num Males | 21 |
| Analysis Type Detail | NMR |
| Release Date | 2022-05-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001378 |
| Project DOI: | doi: 10.21228/M8M12K |
| Project Title: | Untargeted metabolomics following radiation exposure |
| Project Type: | Untargeted metabolic profiling |
| Project Summary: | Comprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours using LC-MS and NMR. |
| Institute: | Institute of Nuclear Medicine and Allied Sciences |
| Department: | Metabolomics facility |
| Laboratory: | Metabolomics facility |
| Last Name: | Rana |
| First Name: | Poonam |
| Address: | DRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India |
| Email: | poonam@inmas.drdo.in |
| Phone: | +911123905319 |
| Funding Source: | INM 313, UGC-JRF |
Subject:
| Subject ID: | SU002252 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA207601 | R_5 Gy 3 | 5Gy Irradiated |
| SA207602 | R_5 Gy 2 | 5Gy Irradiated |
| SA207603 | R_5 Gy 5 | 5Gy Irradiated |
| SA207604 | R_5 Gy 6 | 5Gy Irradiated |
| SA207605 | R_5 Gy 7 | 5Gy Irradiated |
| SA207606 | R_5 Gy 1 | 5Gy Irradiated |
| SA207607 | R_5 Gy 4 | 5Gy Irradiated |
| SA207608 | R_7.5 Gy 3 | 7.5Gy Irradiated |
| SA207609 | R_7.5 Gy 4 | 7.5Gy Irradiated |
| SA207610 | R_7.5 Gy 6 | 7.5Gy Irradiated |
| SA207611 | R_7.5 Gy 7 | 7.5Gy Irradiated |
| SA207612 | R_7.5 Gy 2 | 7.5Gy Irradiated |
| SA207613 | R_7.5 Gy 1 | 7.5Gy Irradiated |
| SA207614 | R_Ctrl 6 | Control |
| SA207615 | R_Ctrl 2 | Control |
| SA207616 | R_Ctrl 1 | Control |
| SA207617 | R_Ctrl 5 | Control |
| SA207618 | R_Ctrl 3 | Control |
| SA207619 | R_Ctrl 4 | Control |
| SA207620 | R_Ctrl 7 | Control |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO002245 |
| Collection Summary: | Animals were acclimatized for 48 hrs in polypropylene cages. Urine samples were collected at 24 hrs post radiation exposure. |
| Sample Type: | Urine |
| Storage Conditions: | -80℃ |
| Collection Tube Temp: | 4℃ |
Treatment:
| Treatment ID: | TR002264 |
| Treatment Summary: | Random categorization was done into three groups: control, 5 Gy (sub-lethal) and 7.5 Gy (lethal) irradiated (seven animals in each group). Mice were kept for 12 hrs light and dark cycle with room temperature (19–23°C) and humidity (45–65%) conditions properly maintained. Mice were exposed to total-body gamma radiation (field size; 35x35 cm) of 5 Gy and 7.5 Gy through Tele 60Co unit γ- irradiation facility (Bhabhatron II, Panacea Medical, Bangalore, India) with dose rate of 1 Gy/min. Control mice were sham irradiated. |
| Treatment: | Radiation exposure |
| Treatment Dose: | 5 Gy and 7.5 Gy Gamma-60Cobalt irradiation |
Sample Preparation:
| Sampleprep ID: | SP002258 |
| Sampleprep Summary: | 150 µl of urine was mixed with 375 µl of deuterated phosphate buffer solution (pH 7.2- 7.4) containing 0.5 mM TSP prepared in D2O. The solution was then transferred to 5 mm NMR tube for data acquisition |
Analysis:
| Analysis ID: | AN003549 |
| Analysis Type: | NMR |
| Results File: | ST002166_AN003549_Results.txt |
| Units: | Integral |
NMR:
| NMR ID: | NM000239 |
| Analysis ID: | AN003549 |
| Instrument Name: | Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600MHz |
