Summary of Study ST002251

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001439. The data can be accessed directly via it's Project DOI: 10.21228/M8QD9Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002251
Study TitleUntargeted metabolomics on plasma from children with asthma, comparing exacerbation-prone to non-exacerbation-prone
Study TypeUntargeted MS
Study SummaryBackground: Some children with asthma remain poorly controlled and have recurrent exacerbations despite treatment with inhaled corticosteroids. Aside from prior exacerbations, there are currently no reliable predictors of exacerbation-prone asthma in these children and limited understanding of potential underlying mechanisms. Objective: We sought to quantify small molecules in the plasma of children with exacerbation- prone asthma through mass spectrometry-based metabolomics. We hypothesized that the plasma metabolome of these children would differ from that of children with non-exacerbation- prone asthma. Methods: Plasma metabolites were extracted from four pediatric asthma cohorts (n=215 total, n=41 with exacerbation-prone asthma) and detected using a ZIC-HILIC column coupled to a Q Exactive HF mass spectrometer. High-confidence annotations were retained for univariate analysis and were confirmed by a sensitivity analysis in subjects on high-dose inhaled corticosteroids. Metabolites that varied by cohort were excluded. Metaboanalyst was used to identify pathways of interest. Concentrations were calculated by reference standardization to NIST SRM 1950. Results: We identified 32 unique, cohort-independent metabolites that differed in children with exacerbation-prone asthma compared to children with non-exacerbation-prone asthma. Comparison of metabolite concentrations to literature-reported values for healthy children revealed that most metabolites were decreased in both asthma groups, but more so in exacerbation-prone asthma. Pathway analysis identified arginine, lysine, and methionine pathways as most impacted. Conclusions: Several plasma metabolites are perturbed in children with exacerbation-prone asthma and are largely related to arginine, lysine, and methionine pathways. While validation is needed, plasma metabolites may be potential biomarkers for exacerbation-prone asthma in children.
Institute
Emory University
DepartmentPediatrics
LaboratoryJoshua Chandler, PhD
Last NameChandler
First NameJoshua
Address2015 Upper Gate Drive NE, Atlanta, GA 30322
Emailjoshua.chandler@emory.edu
Phone404-727-3536
Submit Date2022-07-25
Num Groups2
Total Subjects215
Num Males135
Num Females80
PublicationsJACI-D-22-00220
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-01-02
Release Version1
Joshua Chandler Joshua Chandler
https://dx.doi.org/10.21228/M8QD9Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001439
Project DOI:doi: 10.21228/M8QD9Z
Project Title:Exacerbation-prone pediatric asthma is associated with arginine, lysine, methionine pathway alterations
Project Type:Untargeted MS
Project Summary:Untargeted metabolomics of plasma samples from children with exacerbation-prone asthma compared to children with non-exacerbation-prone asthma
Institute:Emory University
Department:Pediatrics
Laboratory:Joshua Chandler, PhD
Last Name:Chandler
First Name:Joshua
Address:2015 Uppergate Drive NE, Atlanta, GA 30322
Email:joshua.chandler@emory.edu
Phone:404-727-3536
Funding Source:National Institute of Nursing Research; National Center for Advancing Translational Research; National Heart, Lung, and Blood Institute
Publications:JACI-D-22-00220
Contributors:Kirsten A. Cottrill; Susan T. Stephenson; Ahmad F. Mohammad; Susan O. Kim; Nael A. McCarty; Rishikesan Kamaleswaran; Anne M. Fitzpatrick; Joshua D. Chandler

Subject:

Subject ID:SU002337
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:6-17 years
Weight Or Weight Range:Non-exacerbation-prone IQR: 30-68 kg; Exacerbation-prone IQR: 42-63 kg
Height Or Height Range:Non-exacerbation-prone IQR: 131-165 cm; Exacerbation-prone IQR: 140-163 cm
Gender:Male and female
Human Race:18 white, 189 Black, 7 more than one race, 1 Asian
Human Ethnicity:2 Hispanic, 113 Not Hispanic
Human Medications:92 on high-dose inhaled corticosteroids; 94 on long-acting beta adrenergic receptor agonists; 111 on Montelukast
Human Smoking Status:24 exposed to tobacco smoke
Human Inclusion Criteria:1) current or historical evidence of >=12% reversibility in forced expiratory volume in one second (FEV1) relative to baseline after bronchodilator administration; 2) airway hyperresponsiveness to methacholine, with a provocative concentration of methacholine causing a 20% drop in FEV1
Human Exclusion Criteria:1) premature birth before 35 weeks of gestation; 2) other chronic airway disorders that could mimic asthma.
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id ThreeorMoreBursts ICShighdose Study AgeatEnrollment AsthmaDurationYears Sex Ethnicity Race Height Weight BMI LABA Montelukast
SA21679120200724_049_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679220200725_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679320200725_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679420200725_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679520200720_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679620200725_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679720200725_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679820200725_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21679920200724_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680020200720_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680120200718_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680220200715_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680320200718_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680420200718_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680520200720_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680620200718_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680720200718_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680820200720_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21680920200720_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681020200718_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681120200720_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681220200720_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681320200715_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681420200715_005_qcn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681520200715_004_srm1950n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681620200715_006_qcn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681720200719_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681820200718_049_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21681920200718_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682020200715_003_srm1950n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682120200719_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682220200725_044_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682320200715_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682420200725_043_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682520200719_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682620200719_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682720200719_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682820200719_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21682920200725_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683020200715_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683120200719_049_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683220200715_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683320200715_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683420200719_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683520200715_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683620200718_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683720200718_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683820200715_007_qcn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21683920200725_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684020200715_008_qcn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684120200715_009_qcn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684220200719_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684320200715_010_qcn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684420200720_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684520200721_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684620200723_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684720200723_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684820200722_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21684920200722_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685020200722_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685120200722_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685220200721_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685320200721_049_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685420200716_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685520200716_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685620200717_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685720200717_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685820200716_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21685920200716_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686020200722_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686120200722_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686220200723_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686320200723_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686420200723_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686520200723_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686620200722_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686720200723_001_Blank-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686820200723_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21686920200716_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687020200716_049_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687120200716_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687220200719_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687320200722_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687420200716_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687520200722_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687620200717_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687720200717_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687820200721_004_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21687920200721_003_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688020200721_005_QC-ddMS2-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688120200721_006_QC-ddMS2-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688220200717_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688320200717_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688420200721_002_Blank-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688520200722_049_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688620200724_027_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688720200724_028_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688820200717_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21688920200717_049_QC-posn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
SA21689020200720_050_QC-negn/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a
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Collection:

Collection ID:CO002330
Collection Summary:Patients were not fasted prior to blood collection, but did withhold controller medication for 24 hours prior to blood collection. Blood was collected via venipuncture into EDTA-coated tubes. Plasma was isolated via centrifugation and stored at -80 *C.
Sample Type:Blood (plasma)
Storage Conditions:-80℃
Collection Vials:EDTA-coated tubes
Storage Vials:Eppendorf tubes
Collection Tube Temp:Room temperature
Additives:EDTA

Treatment:

Treatment ID:TR002349
Treatment Summary:Plasma samples were not treated with anything prior to metabolite extraction.

Sample Preparation:

Sampleprep ID:SP002343
Sampleprep Summary:50 uL of plasma was mixed with 200 uL of a 50:50 mixture of methanol and acetonitirile, vortexed, incubated on ice for 30 min, then centrifuged at 20,000 g for 10 min at 4 *C. 200 uL of the supernatant was collected for analysis.
Extraction Method:50:50 methanol:acetonitrile for a 1:2:2 samples:methanol:acetonitrile final
Extract Cleanup:centrifuged at 20,000 g for 10 min at 4 *C
Extract Storage:-80℃

Chromatography:

Chromatography ID:CH002727
Chromatography Summary:Equilibration time was 4 min at 90% B, with 3.5 min at 0.35 mL/min, then 0.5 min at 0.25 mL/min. After sample injection, a gradient ran from 90% to 20% B over 17.5 min at 0.25 mL/min, then flow was increased to 0.35 mL/min for 1 min and held at 20% B at 0.35 mL/min for 3.5 min.
Instrument Name:Vanquish Horizon Binary ultrahigh performance liquid chromatography system
Column Name:SeQuant ZIC-HILIC
Column Pressure:15-180 bar
Column Temperature:40
Flow Rate:0.25-0.35 mL/min
Sample Injection:2 uL
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:1.85-14.5 min
Chromatography Type:HILIC

Analysis:

Analysis ID:AN003677
Laboratory Name:Joshua Chandler, PhD
Analysis Type:MS
Acquisition Date:07/15/2020-07/25/2020
Software Version:Compound Discoverer 3.2
Chromatography ID:CH002727
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST002251_AN003677_Results.txt
Units:peak area
  
Analysis ID:AN003678
Laboratory Name:Joshua Chandler, PhD
Analysis Type:MS
Acquisition Date:07/15/2020-07/25/2020
Software Version:Compound Discoverer 3.2
Chromatography ID:CH002727
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST002251_AN003678_Results.txt
Units:peak area
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