Summary of Study ST002256

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001443. The data can be accessed directly via it's Project DOI: 10.21228/M86H66 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002256
Study Title	Metabolomics and metagenomics of pediatric obesity (Serum)
Study Summary	Pediatric obesity has grown as important global health problem in the world. The pediatric obesity affects all the organs and it is closely linked to risks of metabolic diseases such as diabetes, cardiovascular disease, and mental disease. However, the mechanism of both the pediatric obesity and treatment of the obesity remains unclear. Therefore, we investigated metabolomic pathways related to the pediatric obesity and the treatment through metabolomics and metagenomics approaches.
Institute	Seoul National University College of Medicine and Hospital
Last Name	Lee
First Name	Yujin
Address	101, Daehak-ro, Jongno-gu, Seoul, Republic of Korea
Email	yoojinlee@snu.ac.kr
Phone	+821033804686
Submit Date	2022-06-27
Raw Data Available	Yes
Raw Data File Type(s)	smp
Analysis Type Detail	GC-MS
Release Date	2023-01-04
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR001443
Project DOI:	doi: 10.21228/M86H66
Project Title:	Mechanistic study of pediatric obesity through metabolomics and metagenomics
Project Summary:	Pediatric obesity has grown as an important global health problem in the world. Pediatric obesity affects all the organs and it is closely linked to risks of metabolic diseases such as diabetes, cardiovascular disease, and mental disease. Although many researchers reported results about the pediatric obesity study, the mechanism of both pediatric obesity and its treatment remains unclear. Therefore, we investigated the metabolomic pathways related to pediatric obesity and the treatment through metabolomics and metagenomics approaches.
Institute:	Department of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine and Hospital
Last Name:	Lee
First Name:	Yujin
Address:	101 Daehak-ro, Jongno-gu, Seoul 110-799, Korea, Seoul, Seoul, 03080, Korea, South
Email:	yoojinlee@snu.ac.kr
Phone:	+82-10-3380-4686

Subject:

Subject ID:	SU002342
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Gender	Group
SA217107	N14	F	Normal
SA217108	N10	F	Normal
SA217109	N15	F	Normal
SA217110	N16	F	Normal
SA217111	N22	F	Normal
SA217112	N08	F	Normal
SA217113	N12	F	Normal
SA217114	N05	F	Normal
SA217115	N03	F	Normal
SA217116	M07	F	Obe
SA217117	M14	F	Obe
SA217118	M11	F	Obe
SA217119	M10	F	Obe
SA217120	M16	F	Obe
SA217121	M09	F	Obe
SA217122	M41	F	Obe
SA217123	M25	F	Obe
SA217124	M31	F	Obe
SA217125	M06	F	Obe
SA217126	M19	F	Obe
SA217127	M32	F	Obe
SA217128	M29	F	Obe
SA217129	M08	F	Obe
SA217130	M02	F	Obe
SA217131	N17	M	Normal
SA217132	N09	M	Normal
SA217133	N07	M	Normal
SA217134	N18	M	Normal
SA217135	N06	M	Normal
SA217136	N13	M	Normal
SA217137	N19	M	Normal
SA217138	N11	M	Normal
SA217139	N02	M	Normal
SA217140	N01	M	Normal
SA217141	N04	M	Normal
SA217142	N20	M	Normal
SA217143	M33	M	Obe
SA217144	M28	M	Obe
SA217145	M37	M	Obe
SA217146	M42	M	Obe
SA217147	M40	M	Obe
SA217148	M26	M	Obe
SA217149	M36	M	Obe
SA217150	M35	M	Obe
SA217151	M21	M	Obe
SA217152	M12	M	Obe
SA217153	M15	M	Obe
SA217154	M01	M	Obe
SA217155	M03	M	Obe
SA217156	M05	M	Obe
SA217157	M17	M	Obe
SA217158	M18	M	Obe
SA217159	M23	M	Obe
SA217160	M22	M	Obe
SA217161	M04	M	Obe
SA217162	M20	M	Obe
SA217163	M24	M	Obe

Showing results 1 to 57 of 57

Showing results 1 to 57 of 57

Collection:

Collection ID:	CO002335
Collection Summary:	This longitudinal cohort study is an analysis of serum samples collected from obese children before and after a 2-month weight reduction program that consisted of three visits to the hospital.
Sample Type:	Blood (serum)

Treatment:

Treatment ID:	TR002354
Treatment Summary:	Participants completed the questionnaires on general lifestyle (the time spent studying and using electronic devices, the duration and frequency of regular exercise, the presence of easily accessible locations to exercise, and their mode of transportation to school) and eating habits (meal duration, the consumption of late-night snacks, the consumption of breakfast, and the intake of sugar-sweetened beverages) and submitted them at the first hospital visit.

Treatment ID:

TR002354

Treatment Summary:

Participants completed the questionnaires on general lifestyle (the time spent studying and using electronic devices, the duration and frequency of regular exercise, the presence of easily accessible locations to exercise, and their mode of transportation to school) and eating habits (meal duration, the consumption of late-night snacks, the consumption of breakfast, and the intake of sugar-sweetened beverages) and submitted them at the first hospital visit.

Sample Preparation:

Sampleprep ID:	SP002348
Sampleprep Summary:	Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min.

Sampleprep ID:

SP002348

Sampleprep Summary:

Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min.

Combined analysis:

Analysis ID	AN003685
Analysis type	MS
Chromatography type	GC
Chromatography system	Agilent 7890B
Column	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type	EI
MS instrument type	GC-TOF
MS instrument name	Leco Pegasus HT TOF
Ion Mode	NEGATIVE
Units	intensity

Chromatography:

Chromatography ID:	CH002732
Instrument Name:	Agilent 7890B
Column Name:	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Chromatography Type:	GC

MS:

MS ID:	MS003436
Analysis ID:	AN003685
Instrument Name:	Leco Pegasus HT TOF
Instrument Type:	GC-TOF
MS Type:	EI
MS Comments:	The LECO Corporation ChromaTOF®-GC software (v4.72) was used for data acquisition and extraction. This included computation of baseline (offset of 1; above the noise) and automatic entering the number of data points for smoothing. The software's Statistical Compare was used to align peaks. The MS spectral matching was performed using the NIST and Wiley9 libraries. Also, retention index method was used for identification.
Ion Mode:	NEGATIVE