Summary of Study ST002333

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001493. The data can be accessed directly via it's Project DOI: 10.21228/M8R403 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST002333
Study TitleBrain metabonomics of rats in sham, HIE, CO and ASO groups were compared.
Study SummaryTo study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), rats were randomly divided into two groups: Sham group (n=12), HIE group (n=8). After 48 hours of successful modeling, we re-group the HIE group: CO group (n=5) and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil for 30 days, rats in the CO group were treated by an equal volume of corn oil.
Institute
Bao Feng Key Laboratory of Genetics and Metabolism
Last NameChen
First Namexianyang
AddressRoom 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core Zone, Shunyi District, Beijing
Emailcxyibcas@163.com
Phone13681398052
Submit Date2022-10-20
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2026-01-02
Release Version1
xianyang Chen xianyang Chen
https://dx.doi.org/10.21228/M8R403
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001493
Project DOI:doi: 10.21228/M8R403
Project Title:Lipidomic profiling indicate protection of hypoxic-ischemic brain injury on neonatal rats ingesting Acer truncatum Bunge oil
Project Summary:Hypoxic ischemic encephalopathy (HIE) has a great impact on the growth and development of newborns. At present, there is a lack of other effective intervention measures except hypothermia therapy, and the effective rate of hypothermia therapy is less than 50%. Using high-throughput metabonomics, we analyzed the serum and brain tissues of 7-day-old HIE mice, mice fed with Acer truncatum Bunge seed oil (ASO) for 30 days and mice fed with their respective ages. All the results revealed that a variety of metabolites changed significantly during injury and repair, and there were many significant enrichment pathways of designed lipid metabolism and neurotrophic factors. Multi-group comparative analysis identified metabolites that may be related to the injury and repair of hypoxic-ischemic encephalopathy, such as glycerol phospholipids and sphingomyelins. Our results suggest that ASO may be a potential special medical food for ischemic hypoxia newborns.
Institute:Bao Feng Key Laboratory of Genetics and Metabolism
Last Name:Chen
First Name:xianyang
Address:Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core Zone, Shunyi District, Beijing
Email:cxyibcas@163.com
Phone:13681398052

Subject:

Subject ID:SU002422
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA23207320210630_28ASO1
SA23207420210702_28ASO1
SA23207520210702_64ASO2
SA23207620210630_64ASO2
SA23207720210702_18ASO3
SA23207820210630_18ASO3
SA23207920210702_79ASO4
SA23208020210630_79ASO4
SA23208120210630_56ASO5
SA23208220210702_56ASO5
SA23208320210702_76ASO6
SA23208420210630_76ASO6
SA23208520210630_57ASO7
SA23208620210702_57ASO7
SA23208720210630_59ASO8
SA23208820210702_59ASO8
SA23208920210702_27CO1
SA23209020210630_27CO1
SA23209120210630_37CO2
SA23209220210702_37CO2
SA23209320210702_24CO3
SA23209420210630_24CO3
SA23209520210702_74CO4
SA23209620210630_74CO4
SA23209720210702_77CO5
SA23209820210630_77CO5
SA23209920210702_29HIE1
SA23210020210630_29HIE1
SA23210120210630_33HIE2
SA23210220210702_33HIE2
SA23210320210630_12HIE3
SA23210420210702_12HIE3
SA23210520210630_61HIE4
SA23210620210702_61HIE4
SA23210720210702_63HIE5
SA23210820210630_63HIE5
SA23210920210630_83HIE6
SA23211020210702_83HIE6
SA23211120210702_78HIE7
SA23211220210630_78HIE7
SA23211320210702_72HIE8
SA23211420210630_72HIE8
SA23211520210630_16shaHIE1
SA23211620210702_16shaHIE1
SA23211720210702_14shaHIE10
SA23211820210630_14shaHIE10
SA23211920210702_50shaHIE11
SA23212020210630_50shaHIE11
SA23212120210630_19shaHIE12
SA23212220210702_19shaHIE12
SA23212320210702_67shaHIE2
SA23212420210630_67shaHIE2
SA23212520210702_39shaHIE3
SA23212620210630_39shaHIE3
SA23212720210630_40shaHIE4
SA23212820210702_40shaHIE4
SA23212920210702_44shaHIE5
SA23213020210630_44shaHIE5
SA23213120210630_41shaHIE6
SA23213220210702_41shaHIE6
SA23213320210630_65shaHIE7
SA23213420210702_65shaHIE7
SA23213520210630_35shaHIE8
SA23213620210702_35shaHIE8
SA23213720210702_20shaHIE9
SA23213820210630_20shaHIE9
Showing results 1 to 66 of 66

Collection:

Collection ID:CO002415
Collection Summary:Take the whole brain according to the experimental purpose, and then immediately wash the blood residue on the tissue with precooled normal saline or deionized water. Divide the homogenized brain tissue into sterile tubes for every 250mg of tissue samples. Take multiple tubes for each sample for backup, mark them, seal them with sealing membrane, and freeze them at - 80 ℃.
Sample Type:Brain

Treatment:

Treatment ID:TR002434
Treatment Summary:To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), rats were randomly divided into two groups: Sham group (n=12), HIE group (n=8). After 48 hours of successful modeling, we re-group the HIE group: CO group (n=5) and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil for 30 days, rats in the CO group were treated by an equal volume of corn oil25. Based on the individual recommended intake NA of 0.3 g/kg/d, the equivalent dose of the drug was calculated according to the bodyweight of the human and the animal, in which the daily dose of the rat was equivalent to six times that of adults.

Sample Preparation:

Sampleprep ID:SP002428
Sampleprep Summary:We use isopropanol (IPA) to extract lipids from serum and brain tissue. In brief, 100mg of brain tissue homogenized was extracted with 600 μL of precooled IPA temperature of 4°C, vortexed for 1 minute and incubated for 10 minutes at room temperature; the extracted mixture was stored overnight at -20 ° C. Samples were centrifuged for 20 minutes at 14000 rpm, and the supernatants were transferred into a new centrifuge tube and diluted to 1:10 with IPA/ACN/H2O (2.5:1:1, v: v: v). Before LC/MS analysis, the sample was stored at -80°C.

Combined analysis:

Analysis ID AN003812 AN003813
Chromatography ID CH002820 CH002820
MS ID MS003554 MS003555
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity I-Class Waters Acquity I-Class
Column Waters Acquity BEH C8 (100 x 2.1mm,1.7um) Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Xevo-G2-XS Waters Xevo-G2-XS
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH002820
Chromatography Summary:ACQUITYUPLC I-CLASS (Waters) and XEVO-G2XS quadrupole time-of-flight (QTOF) mass spectrometry (Waters) with ESI were used to analyze the samples.
Methods Filename:Chrom__methods_.pdf
Instrument Name:Waters Acquity I-Class
Column Name:Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Flow Rate:0.4 mL/min
Solvent A:60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS003554
Analysis ID:AN003812
Instrument Name:Waters Xevo-G2-XS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS003555
Analysis ID:AN003813
Instrument Name:Waters Xevo-G2-XS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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