Summary of Study ST002483

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001493. The data can be accessed directly via it's Project DOI: 10.21228/M8R403 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST002483
Study TitleBrain metabonomics of rats in CO and ASO groups from 4500m were compared.
Study SummaryTo study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), After 48 hours of successful modeling, we re-group the HIE group: CO group (n=8) and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil for 30 days, rats in the CO group were treated by an equal volume of corn oil.
Institute
Bao Feng Key Laboratory of Genetics and Metabolism
Last NameChen
First NameXianyang
AddressRoom 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core Zone, Shunyi District, Beijing
Emailcxyibcas@163.com
Phone13681398052
Submit Date2022-12-27
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2026-01-02
Release Version1
Xianyang Chen Xianyang Chen
https://dx.doi.org/10.21228/M8R403
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001493
Project DOI:doi: 10.21228/M8R403
Project Title:Lipidomic profiling indicate protection of hypoxic-ischemic brain injury on neonatal rats ingesting Acer truncatum Bunge oil
Project Summary:Hypoxic ischemic encephalopathy (HIE) has a great impact on the growth and development of newborns. At present, there is a lack of other effective intervention measures except hypothermia therapy, and the effective rate of hypothermia therapy is less than 50%. Using high-throughput metabonomics, we analyzed the serum and brain tissues of 7-day-old HIE mice, mice fed with Acer truncatum Bunge seed oil (ASO) for 30 days and mice fed with their respective ages. All the results revealed that a variety of metabolites changed significantly during injury and repair, and there were many significant enrichment pathways of designed lipid metabolism and neurotrophic factors. Multi-group comparative analysis identified metabolites that may be related to the injury and repair of hypoxic-ischemic encephalopathy, such as glycerol phospholipids and sphingomyelins. Our results suggest that ASO may be a potential special medical food for ischemic hypoxia newborns.
Institute:Bao Feng Key Laboratory of Genetics and Metabolism
Last Name:Chen
First Name:xianyang
Address:Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core Zone, Shunyi District, Beijing
Email:cxyibcas@163.com
Phone:13681398052

Subject:

Subject ID:SU002573
Subject Type:Mammal
Subject Species:Rattus norvegicus
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA24859320220818_27H01
SA24859420220819_27H01
SA24859520220818_21H02
SA24859620220819_21H02
SA24859720220819_23H03
SA24859820220818_23H03
SA24859920220819_14H04
SA24860020220818_14H04
SA24860120220819_18H05
SA24860220220818_18H05
SA24860320220818_12H06
SA24860420220819_12H06
SA24860520220818_16H07
SA24860620220819_16H07
SA24860720220819_25H08
SA24860820220818_25H08
SA24860920220819_13HS1
SA24861020220818_13HS1
SA24861120220818_22HS2
SA24861220220819_22HS2
SA24861320220819_15HS3
SA24861420220818_15HS3
SA24861520220818_19HS4
SA24861620220819_19HS4
SA24861720220818_17HS5
SA24861820220819_17HS5
SA24861920220818_26HS6
SA24862020220819_26HS6
SA24862120220818_28HS7
SA24862220220819_28HS7
SA24862320220818_24HS8
SA24862420220819_24HS8
Showing results 1 to 32 of 32

Collection:

Collection ID:CO002566
Collection Summary:Take the whole brain according to the experimental purpose, and then immediately wash the blood residue on the tissue with precooled normal saline or deionized water. Divide the homogenized brain tissue into sterile tubes for every 250mg of tissue samples. Take multiple tubes for each sample for backup, mark them, seal them with sealing membrane, and freeze them at - 80 ℃.
Sample Type:Brain
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002585
Treatment Summary:To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI). After 48 hours of successful modeling, we re-group the HIE group: CO group (n=8) and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil for 30 days, rats in the CO group were treated by an equal volume of corn oil. Based on the individual recommended intake NA of 0.3 g/kg/d, the equivalent dose of the drug was calculated according to the bodyweight of the human and the animal, in which the daily dose of the rat was equivalent to six times that of adults.

Sample Preparation:

Sampleprep ID:SP002579
Sampleprep Summary:We use isopropanol (IPA) to extract lipids from serum and brain tissue. In brief, 100mg of brain tissue homogenized was extracted with 600 μL of precooled IPA temperature of 4°C, vortexed for 1 minute and incubated for 10 minutes at room temperature; the extracted mixture was stored overnight at -20 ° C. Samples were centrifuged for 20 minutes at 14000 rpm, and the supernatants were transferred into a new centrifuge tube and diluted to 1:10 with IPA/ACN/H2O (2.5:1:1, v: v: v). Before LC/MS analysis, the sample was stored at -80°C.

Combined analysis:

Analysis ID AN004053 AN004054
Chromatography ID CH003000 CH003000
MS ID MS003800 MS003801
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity I-Class Waters Acquity I-Class
Column Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) Waters Acquity BEH C8 (100 x 2.1mm, 1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Xevo-G2-XS Waters Xevo-G2-XS
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH003000
Methods Filename:Chrom__methods_.pdf
Instrument Name:Waters Acquity I-Class
Column Name:Waters Acquity BEH C8 (100 x 2.1mm, 1.7um)
Column Temperature:550 ℃
Flow Gradient:The injected 1 µL sample was initially eluted with 40% B, graded linearly to 43% B in 2 minutes, and then increased to 50% B in 0.1 minutes. In the next 9.9 minutes, the gradient was further increased to 54% B and then increased to 70% in 0.1 minutes. In the last part of the gradient, the amount of B was increased to 99% in 5.9 minutes. Finally, the column was balanced for 1.9 minutes before the next injection, and solution B returned to 40% in 0.1 minutes.
Flow Rate:0.4 mL/min
Solvent A:60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS003800
Analysis ID:AN004053
Instrument Name:Waters Xevo-G2-XS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS003801
Analysis ID:AN004054
Instrument Name:Waters Xevo-G2-XS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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