Summary of Study ST002593

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002593
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Gastrocnemius - Targeted Ethanolamides
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Emory University
DepartmentBiochemistry
LaboratoryIntegrated Lipidomics and Metabolomics Core
Last NameOrtlund
First NameEric
Address1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Emaileortlun@emory.edu
Phone(404) 727-5014
Submit Date2023-04-27
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Eric Ortlund Eric Ortlund
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002695
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA25955190265015511Control 8 weeks of training or control time Female
SA25955290245015511Control 8 weeks of training or control time Female
SA25955390248015511Control 8 weeks of training or control time Female
SA25955490266015511Control 8 weeks of training or control time Female
SA25955590252015511Control 8 weeks of training or control time Female
SA25955690217015511Control 8 weeks of training or control time Male
SA25955790239015511Control 8 weeks of training or control time Male
SA25955890237015511Control 8 weeks of training or control time Male
SA25955990229015511Control 8 weeks of training or control time Male
SA25956090232015511Control 8 weeks of training or control time Male
SA259561QC_blank_69QC-Blank 0 hour -
SA259562QC_blank_68QC-Blank 0 hour -
SA259563QC_blank_79QC-Blank 0 hour -
SA259564QC_blank_78QC-Blank 0 hour -
SA259565QC_blank_65QC-Blank 0 hour -
SA259566QC_blank_4QC-Blank 0 hour -
SA259567QC_Standard_71QC-ExternalStandard 0 hour -
SA259568QC_Standard_74QC-ExternalStandard 0 hour -
SA259569QC_Standard_75QC-ExternalStandard 0 hour -
SA259570QC_Standard_76QC-ExternalStandard 0 hour -
SA259571QC_Standard_77QC-ExternalStandard 0 hour -
SA259572QC_Standard_73QC-ExternalStandard 0 hour -
SA259573QC_Standard_72QC-ExternalStandard 0 hour -
SA259574QC_Standard_70QC-ExternalStandard 0 hour -
SA259575QC_62QC-Pooled 0 hour -
SA259576QC_51QC-Pooled 0 hour -
SA259577QC_67QC-Pooled 0 hour -
SA259578QC_40QC-Pooled 0 hour -
SA259579QC_7QC-Pooled 0 hour -
SA259580QC_29QC-Pooled 0 hour -
SA259581QC_18QC-Pooled 0 hour -
SA259582QC_prerun_1QC-PreRun 0 hour -
SA259583QC_prerun_2QC-PreRun 0 hour -
SA259584QC_prerun_3QC-PreRun 0 hour -
SA259585G_QC_1_63QC-Reference 0 hour -
SA259586G_QC_2_6QC-Reference 0 hour -
SA259587QC_SRMQC-Reference 0 hour -
SA259588G_QC_1_5QC-Reference 0 hour -
SA259589G_QC_2_64QC-Reference 0 hour -
SA25959090571015511Training 1 week of training or control time Female
SA25959190564015511Training 1 week of training or control time Female
SA25959290559015511Training 1 week of training or control time Female
SA25959390567015511Training 1 week of training or control time Female
SA25959490560015511Training 1 week of training or control time Female
SA25959590423015511Training 1 week of training or control time Male
SA25959690430015511Training 1 week of training or control time Male
SA25959790426015511Training 1 week of training or control time Male
SA25959890421015511Training 1 week of training or control time Male
SA25959990422015511Training 1 week of training or control time Male
SA25960090587015511Training 2 weeks of training Female
SA25960190581015511Training 2 weeks of training Female
SA25960290576015511Training 2 weeks of training Female
SA25960390585015511Training 2 weeks of training Female
SA25960490578015511Training 2 weeks of training Female
SA25960590449015511Training 2 weeks of training Male
SA25960690444015511Training 2 weeks of training Male
SA25960790441015511Training 2 weeks of training Male
SA25960890450015511Training 2 weeks of training Male
SA25960990439015511Training 2 weeks of training Male
SA25961090412015511Training 4 weeks of training Female
SA25961190410015511Training 4 weeks of training Female
SA25961290406015511Training 4 weeks of training Female
SA25961390420015511Training 4 weeks of training Female
SA25961490416015511Training 4 weeks of training Female
SA25961590292015511Training 4 weeks of training Male
SA25961690280015511Training 4 weeks of training Male
SA25961790283015511Training 4 weeks of training Male
SA25961890281015511Training 4 weeks of training Male
SA25961990289015511Training 4 weeks of training Male
SA25962090254015511Training 8 weeks of training or control time Female
SA25962190259015511Training 8 weeks of training or control time Female
SA25962290258015511Training 8 weeks of training or control time Female
SA25962390267015511Training 8 weeks of training or control time Female
SA25962490251015511Training 8 weeks of training or control time Female
SA25962590227015511Training 8 weeks of training or control time Male
SA25962690225015511Training 8 weeks of training or control time Male
SA25962790223015511Training 8 weeks of training or control time Male
SA25962890222015511Training 8 weeks of training or control time Male
SA25962990218015511Training 8 weeks of training or control time Male
Showing results 1 to 79 of 79

Collection:

Collection ID:CO002688
Collection Summary:-
Sample Type:Gastrocnemius

Treatment:

Treatment ID:TR002707
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002701
Sampleprep Summary:purification of oxylipids with SPE column
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004265
Analysis type MS
Chromatography type Reversed phase
Chromatography system ExionLC AC
Column ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 Qtrap
Ion Mode POSITIVE
Units ng/uL

Chromatography:

Chromatography ID:CH003171
Chromatography Summary:Lipids were separated by liquid chromatography with 20 minutes gradient
Methods Filename:pass1b_oxylipneg_etamidpos_methods.pdf
Instrument Name:ExionLC AC
Column Name:ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @ 0.5 ml/min
Flow Rate:0.5 ml/min
Solvent A:100% water; 10 mM ammonium acetate
Solvent B:100% acetonitrile; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004012
Analysis ID:AN004265
Instrument Name:ABI Sciex 5500 Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Multiple reaction monitoring mode (MRM)
Ion Mode:POSITIVE
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