Summary of Study ST002596

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002596
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Gastrocnemius - Targeted Oxylipins
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Emory University
DepartmentBiochemistry
LaboratoryIntegrated Lipidomics and Metabolomics Core
Last NameOrtlund
First NameEric
Address1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Emaileortlun@emory.edu
Phone(404) 727-5014
Submit Date2023-04-25
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Eric Ortlund Eric Ortlund
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002698
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA25974690265015511Control 8 weeks of training or control time Female
SA25974790245015511Control 8 weeks of training or control time Female
SA25974890248015511Control 8 weeks of training or control time Female
SA25974990266015511Control 8 weeks of training or control time Female
SA25975090252015511Control 8 weeks of training or control time Female
SA25975190217015511Control 8 weeks of training or control time Male
SA25975290239015511Control 8 weeks of training or control time Male
SA25975390237015511Control 8 weeks of training or control time Male
SA25975490229015511Control 8 weeks of training or control time Male
SA25975590232015511Control 8 weeks of training or control time Male
SA259756QC_blank_69QC-Blank 0 hour -
SA259757QC_blank_68QC-Blank 0 hour -
SA259758QC_blank_79QC-Blank 0 hour -
SA259759QC_blank_78QC-Blank 0 hour -
SA259760QC_blank_65QC-Blank 0 hour -
SA259761QC_blank_4QC-Blank 0 hour -
SA259762QC_Standard_71QC-ExternalStandard 0 hour -
SA259763QC_Standard_74QC-ExternalStandard 0 hour -
SA259764QC_Standard_75QC-ExternalStandard 0 hour -
SA259765QC_Standard_76QC-ExternalStandard 0 hour -
SA259766QC_Standard_77QC-ExternalStandard 0 hour -
SA259767QC_Standard_73QC-ExternalStandard 0 hour -
SA259768QC_Standard_72QC-ExternalStandard 0 hour -
SA259769QC_Standard_70QC-ExternalStandard 0 hour -
SA259770QC_62QC-Pooled 0 hour -
SA259771QC_51QC-Pooled 0 hour -
SA259772QC_67QC-Pooled 0 hour -
SA259773QC_40QC-Pooled 0 hour -
SA259774QC_7QC-Pooled 0 hour -
SA259775QC_29QC-Pooled 0 hour -
SA259776QC_18QC-Pooled 0 hour -
SA259777QC_prerun_1QC-PreRun 0 hour -
SA259778QC_prerun_2QC-PreRun 0 hour -
SA259779QC_prerun_3QC-PreRun 0 hour -
SA259780G_QC_1_63QC-Reference 0 hour -
SA259781G_QC_2_6QC-Reference 0 hour -
SA259782QC_SRMQC-Reference 0 hour -
SA259783G_QC_1_5QC-Reference 0 hour -
SA259784G_QC_2_64QC-Reference 0 hour -
SA25978590571015511Training 1 week of training or control time Female
SA25978690564015511Training 1 week of training or control time Female
SA25978790559015511Training 1 week of training or control time Female
SA25978890567015511Training 1 week of training or control time Female
SA25978990560015511Training 1 week of training or control time Female
SA25979090423015511Training 1 week of training or control time Male
SA25979190430015511Training 1 week of training or control time Male
SA25979290426015511Training 1 week of training or control time Male
SA25979390421015511Training 1 week of training or control time Male
SA25979490422015511Training 1 week of training or control time Male
SA25979590587015511Training 2 weeks of training Female
SA25979690581015511Training 2 weeks of training Female
SA25979790576015511Training 2 weeks of training Female
SA25979890585015511Training 2 weeks of training Female
SA25979990578015511Training 2 weeks of training Female
SA25980090449015511Training 2 weeks of training Male
SA25980190444015511Training 2 weeks of training Male
SA25980290441015511Training 2 weeks of training Male
SA25980390450015511Training 2 weeks of training Male
SA25980490439015511Training 2 weeks of training Male
SA25980590412015511Training 4 weeks of training Female
SA25980690410015511Training 4 weeks of training Female
SA25980790406015511Training 4 weeks of training Female
SA25980890420015511Training 4 weeks of training Female
SA25980990416015511Training 4 weeks of training Female
SA25981090292015511Training 4 weeks of training Male
SA25981190280015511Training 4 weeks of training Male
SA25981290283015511Training 4 weeks of training Male
SA25981390281015511Training 4 weeks of training Male
SA25981490289015511Training 4 weeks of training Male
SA25981590254015511Training 8 weeks of training or control time Female
SA25981690259015511Training 8 weeks of training or control time Female
SA25981790258015511Training 8 weeks of training or control time Female
SA25981890267015511Training 8 weeks of training or control time Female
SA25981990251015511Training 8 weeks of training or control time Female
SA25982090227015511Training 8 weeks of training or control time Male
SA25982190225015511Training 8 weeks of training or control time Male
SA25982290223015511Training 8 weeks of training or control time Male
SA25982390222015511Training 8 weeks of training or control time Male
SA25982490218015511Training 8 weeks of training or control time Male
Showing results 1 to 79 of 79

Collection:

Collection ID:CO002691
Collection Summary:-
Sample Type:Gastrocnemius

Treatment:

Treatment ID:TR002710
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002704
Sampleprep Summary:purification of oxylipids with SPE column
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004268
Analysis type MS
Chromatography type Reversed phase
Chromatography system ExionLC AC
Column ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 Qtrap
Ion Mode NEGATIVE
Units ng/uL

Chromatography:

Chromatography ID:CH003174
Chromatography Summary:Lipids were separated by liquid chromatography with 20 minutes gradient
Methods Filename:pass1b_oxylipneg_etamidpos_methods.pdf
Instrument Name:ExionLC AC
Column Name:ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @ 0.5 ml/min
Flow Rate:0.5 ml/min
Solvent A:100% water; 10 mM ammonium acetate
Solvent B:100% acetonitrile; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004015
Analysis ID:AN004268
Instrument Name:ABI Sciex 5500 Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Multiple reaction monitoring mode (MRM)
Ion Mode:NEGATIVE
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