Summary of Study ST002612

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002612
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Liver - Targeted Amines
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Mayo Clinic
DepartmentInternal Medicine
LaboratoryMayo Clinic Metabolomics Resource Core (MCMRC)
Last NameNair
First NameK. Sreekumaran
Address200 1st St SW, Rochester, MN 55902
Emailnair@mayo.edu
Phone(507) 284-2511
Submit Date2023-04-28
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
K. Sreekumaran Nair K. Sreekumaran Nair
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002714
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26078290266016813Control 8 weeks of training or control time Female
SA26078390245016813Control 8 weeks of training or control time Female
SA26078490248016813Control 8 weeks of training or control time Female
SA26078590265016813Control 8 weeks of training or control time Female
SA26078690252016813Control 8 weeks of training or control time Female
SA26078790239016813Control 8 weeks of training or control time Male
SA26078890237016813Control 8 weeks of training or control time Male
SA26078990229016813Control 8 weeks of training or control time Male
SA26079090232016813Control 8 weeks of training or control time Male
SA26079190217016813Control 8 weeks of training or control time Male
SA26079290559016813Training 1 week of training or control time Female
SA26079390560016813Training 1 week of training or control time Female
SA26079490564016813Training 1 week of training or control time Female
SA26079590567016813Training 1 week of training or control time Female
SA26079690571016813Training 1 week of training or control time Female
SA26079790430016813Training 1 week of training or control time Male
SA26079890421016813Training 1 week of training or control time Male
SA26079990423016813Training 1 week of training or control time Male
SA26080090426016813Training 1 week of training or control time Male
SA26080190422016813Training 1 week of training or control time Male
SA26080290587016813Training 2 weeks of training Female
SA26080390585016813Training 2 weeks of training Female
SA26080490576016813Training 2 weeks of training Female
SA26080590578016813Training 2 weeks of training Female
SA26080690581016813Training 2 weeks of training Female
SA26080790449016813Training 2 weeks of training Male
SA26080890450016813Training 2 weeks of training Male
SA26080990444016813Training 2 weeks of training Male
SA26081090441016813Training 2 weeks of training Male
SA26081190439016813Training 2 weeks of training Male
SA26081290406016813Training 4 weeks of training Female
SA26081390420016813Training 4 weeks of training Female
SA26081490410016813Training 4 weeks of training Female
SA26081590412016813Training 4 weeks of training Female
SA26081690416016813Training 4 weeks of training Female
SA26081790280016813Training 4 weeks of training Male
SA26081890281016813Training 4 weeks of training Male
SA26081990289016813Training 4 weeks of training Male
SA26082090292016813Training 4 weeks of training Male
SA26082190283016813Training 4 weeks of training Male
SA26082290251016813Training 8 weeks of training or control time Female
SA26082390254016813Training 8 weeks of training or control time Female
SA26082490267016813Training 8 weeks of training or control time Female
SA26082590258016813Training 8 weeks of training or control time Female
SA26082690259016813Training 8 weeks of training or control time Female
SA26082790222016813Training 8 weeks of training or control time Male
SA26082890218016813Training 8 weeks of training or control time Male
SA26082990225016813Training 8 weeks of training or control time Male
SA26083090227016813Training 8 weeks of training or control time Male
SA26083190223016813Training 8 weeks of training or control time Male
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Collection:

Collection ID:CO002707
Collection Summary:-
Sample Type:Liver

Treatment:

Treatment ID:TR002724
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002720
Sampleprep Summary:homogenized in PBS, isotope IS added, deproteinized
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004284
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity UPLC
Column Waters Acquity UPLC BEH C8 (150 x 2.1mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo TSQ Quantiva
Ion Mode POSITIVE
Units nano moles per vial

Chromatography:

Chromatography ID:CH003190
Chromatography Summary:Analytes were chromatographically separated with 40 minutes LC gradient
Methods Filename:pass1b_amines_methods.pdf
Instrument Name:Waters Acquity UPLC
Column Name:Waters Acquity UPLC BEH C8 (150 x 2.1mm, 1.7um)
Column Temperature:43C
Flow Gradient:t=0 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=1 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=2 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=5.5 min: 98.1%/1.9% (%A/%B) @ 0.4 ml/min | t=6.5 min: 98%/2% (%A/%B) @ 0.4 ml/min | t=10 min: 97.5%/2.4% (%A/%B) @ 0.4 ml/min | t=12 min: 96%/4% (%A/%B) @ 0.4 ml/min | t=20 min: 88%/12% (%A/%B) @ 0.4 ml/min | t=27 min: 86.5%/13.5% (%A/%B) @ 0.4 ml/min | t=31 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=32 min: 2%/98% (%A/%B) @ 0.5 ml/min | t=35 min: 2%/98% (%A/%B) @ 0.5 ml/min | t=35.5 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=39 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min
Flow Rate:0.4 ml/min; 0.5 ml/min
Solvent A:1% acetonitrile/99% water; 0.1% formic acid
Solvent B:1% water/99% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS004031
Analysis ID:AN004284
Instrument Name:Thermo TSQ Quantiva
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Multiple reaction monitoring mode (MRM); peak integration, concentrations calculated against multi-point standard curves;
Ion Mode:POSITIVE
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