Summary of Study ST002623

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002623
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:White Adipose - Targeted Ethanolamides
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Emory University
DepartmentBiochemistry
LaboratoryIntegrated Lipidomics and Metabolomics Core
Last NameOrtlund
First NameEric
Address1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Emaileortlun@emory.edu
Phone(404) 727-5014
Submit Date2023-04-28
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Eric Ortlund Eric Ortlund
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002725
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26137190266017010Control 8 weeks of training or control time Female
SA26137290245017010Control 8 weeks of training or control time Female
SA26137390265017010Control 8 weeks of training or control time Female
SA26137490252017010Control 8 weeks of training or control time Female
SA26137590248017010Control 8 weeks of training or control time Female
SA26137690217017010Control 8 weeks of training or control time Male
SA26137790239017010Control 8 weeks of training or control time Male
SA26137890232017010Control 8 weeks of training or control time Male
SA26137990229017010Control 8 weeks of training or control time Male
SA26138090237017010Control 8 weeks of training or control time Male
SA261381QC_blank_65QC-Blank 0 hour -
SA261382QC_blank_68QC-Blank 0 hour -
SA261383QC_blank_78QC-Blank 0 hour -
SA261384QC_blank_5QC-Blank 0 hour -
SA261385QC_blank_79QC-Blank 0 hour -
SA261386QC_blank_69QC-Blank 0 hour -
SA261387QC_Standard_70QC-ExternalStandard 0 hour -
SA261388QC_Standard_73QC-ExternalStandard 0 hour -
SA261389QC_Standard_74QC-ExternalStandard 0 hour -
SA261390QC_Standard_76QC-ExternalStandard 0 hour -
SA261391QC_Standard_72QC-ExternalStandard 0 hour -
SA261392QC_Standard_77QC-ExternalStandard 0 hour -
SA261393QC_Standard_71QC-ExternalStandard 0 hour -
SA261394QC_Standard_75QC-ExternalStandard 0 hour -
SA261395QC_62QC-Pooled 0 hour -
SA261396QC_67QC-Pooled 0 hour -
SA261397QC_51QC-Pooled 0 hour -
SA261398QC_40QC-Pooled 0 hour -
SA261399QC_18QC-Pooled 0 hour -
SA261400QC_7QC-Pooled 0 hour -
SA261401QC_29QC-Pooled 0 hour -
SA261402QC_prerun_1QC-PreRun 0 hour -
SA261403QC_prerun_3QC-PreRun 0 hour -
SA261404QC_prerun_2QC-PreRun 0 hour -
SA261405QC_SRMQC-Reference 0 hour -
SA261406G_QC_2_64QC-Reference 0 hour -
SA261407G_QC_2_6QC-Reference 0 hour -
SA261408G_QC_1_4QC-Reference 0 hour -
SA261409G_QC_1_63QC-Reference 0 hour -
SA26141090571017010Training 1 week of training or control time Female
SA26141190559017010Training 1 week of training or control time Female
SA26141290567017010Training 1 week of training or control time Female
SA26141390564017010Training 1 week of training or control time Female
SA26141490560017010Training 1 week of training or control time Female
SA26141590423017010Training 1 week of training or control time Male
SA26141690421017010Training 1 week of training or control time Male
SA26141790430017010Training 1 week of training or control time Male
SA26141890422017010Training 1 week of training or control time Male
SA26141990426017010Training 1 week of training or control time Male
SA26142090581017010Training 2 weeks of training Female
SA26142190587017010Training 2 weeks of training Female
SA26142290578017010Training 2 weeks of training Female
SA26142390576017010Training 2 weeks of training Female
SA26142490585017010Training 2 weeks of training Female
SA26142590450017010Training 2 weeks of training Male
SA26142690444017010Training 2 weeks of training Male
SA26142790439017010Training 2 weeks of training Male
SA26142890441017010Training 2 weeks of training Male
SA26142990449017010Training 2 weeks of training Male
SA26143090412017010Training 4 weeks of training Female
SA26143190420017010Training 4 weeks of training Female
SA26143290406017010Training 4 weeks of training Female
SA26143390410017010Training 4 weeks of training Female
SA26143490416017010Training 4 weeks of training Female
SA26143590281017010Training 4 weeks of training Male
SA26143690283017010Training 4 weeks of training Male
SA26143790289017010Training 4 weeks of training Male
SA26143890280017010Training 4 weeks of training Male
SA26143990292017010Training 4 weeks of training Male
SA26144090251017010Training 8 weeks of training or control time Female
SA26144190254017010Training 8 weeks of training or control time Female
SA26144290267017010Training 8 weeks of training or control time Female
SA26144390259017010Training 8 weeks of training or control time Female
SA26144490258017010Training 8 weeks of training or control time Female
SA26144590223017010Training 8 weeks of training or control time Male
SA26144690222017010Training 8 weeks of training or control time Male
SA26144790218017010Training 8 weeks of training or control time Male
SA26144890225017010Training 8 weeks of training or control time Male
SA26144990227017010Training 8 weeks of training or control time Male
Showing results 1 to 79 of 79

Collection:

Collection ID:CO002718
Collection Summary:-
Sample Type:White adipose

Treatment:

Treatment ID:TR002734
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002731
Sampleprep Summary:purification of oxylipids with SPE column
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004295
Analysis type MS
Chromatography type Reversed phase
Chromatography system ExionLC AC
Column ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 Qtrap
Ion Mode POSITIVE
Units ng/uL

Chromatography:

Chromatography ID:CH003201
Chromatography Summary:Lipids were separated by liquid chromatography with 20 minutes gradient
Methods Filename:pass1b_oxylipneg_etamidpos_methods.pdf
Instrument Name:ExionLC AC
Column Name:ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @ 0.5 ml/min
Flow Rate:0.5 ml/min
Solvent A:100% water; 10 mM ammonium acetate
Solvent B:100% acetonitrile; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004042
Analysis ID:AN004295
Instrument Name:ABI Sciex 5500 Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Multiple reaction monitoring mode (MRM)
Ion Mode:POSITIVE
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