Summary of Study ST002643

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002643
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Gastrocnemius - Untargeted Ion-Pair Negative
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameBurant
First NameCharles
Address6120 Brehm Tower,1000 Wall St., Ann Arbor, MI, 48105-5714
Emailburantc@med.umich.edu
Phone(734) 232-0842
Submit Date2023-05-01
Num Groups65
Total Subjects50
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Charles Burant Charles Burant
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002745
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26280290245015509Control 8 weeks of training or control time Female
SA26280390248015509Control 8 weeks of training or control time Female
SA26280490252015509Control 8 weeks of training or control time Female
SA26280590265015509Control 8 weeks of training or control time Female
SA26280690266015509Control 8 weeks of training or control time Female
SA26280790232015509Control 8 weeks of training or control time Male
SA26280890237015509Control 8 weeks of training or control time Male
SA26280990239015509Control 8 weeks of training or control time Male
SA26281090217015509Control 8 weeks of training or control time Male
SA26281190229015509Control 8 weeks of training or control time Male
SA262812CS00000PB-02QC-Blank 0 hour -
SA262813CS00000PB-03QC-Blank 0 hour -
SA262814CS00000PB-04QC-Blank 0 hour -
SA262815CS00000SB-04QC-Blank 0 hour -
SA262816CS00000SB-02QC-Blank 0 hour -
SA262817CS00000SB-03QC-Blank 0 hour -
SA262818CS00000PB-01QC-Blank 0 hour -
SA262819CS00000MP-02QC-DriftCorrection 0 hour -
SA262820CS00000MP-09QC-DriftCorrection 0 hour -
SA262821CS00000MP-05QC-DriftCorrection 0 hour -
SA262822CS00000MP-03QC-DriftCorrection 0 hour -
SA262823CS00000MP-04QC-DriftCorrection 0 hour -
SA262824CS00000MP-06QC-DriftCorrection 0 hour -
SA262825CS00000MP-08QC-DriftCorrection 0 hour -
SA262826CS00000MP-07QC-DriftCorrection 0 hour -
SA262827CS000QCMP-02QC-Pooled 0 hour -
SA262828CS000QCMP-03QC-Pooled 0 hour -
SA262829CS0000OP0-03QC-Pooled 0 hour -
SA262830CS0000OP0-02QC-Pooled 0 hour -
SA262831CS000QCMP-01QC-Pooled 0 hour -
SA262832CS0000OP0-07QC-Pooled 0 hour -
SA262833CS0000OP0-04QC-Pooled 0 hour -
SA262834CS0000OP0-05QC-Pooled 0 hour -
SA262835CS0000OP0-06QC-Pooled 0 hour -
SA262836CS0000OP0-01QC-Pooled 0 hour -
SA262837CS000QCMP-04QC-Pooled 0 hour -
SA262838CS0UMRG01-01QC-ReCAS 0 hour -
SA262839CS0UMRG01-03QC-ReCAS 0 hour -
SA262840CS0UMRG01-04QC-ReCAS 0 hour -
SA262841CS0UMRG01-02QC-ReCAS 0 hour -
SA262842CSMR80008-02QC-Reference 0 hour -
SA262843CSMR80008-01QC-Reference 0 hour -
SA262844CSMR80008-03QC-Reference 0 hour -
SA262845CSMR80008-04QC-Reference 0 hour -
SA262846CSMR80009-03QC-Reference 0 hour -
SA262847CSMR80009-04QC-Reference 0 hour -
SA262848CSMR80009-02QC-Reference 0 hour -
SA262849CSMR80009-01QC-Reference 0 hour -
SA26285090567015509Training 1 week of training or control time Female
SA26285190559015509Training 1 week of training or control time Female
SA26285290564015509Training 1 week of training or control time Female
SA26285390571015509Training 1 week of training or control time Female
SA26285490560015509Training 1 week of training or control time Female
SA26285590430015509Training 1 week of training or control time Male
SA26285690422015509Training 1 week of training or control time Male
SA26285790426015509Training 1 week of training or control time Male
SA26285890421015509Training 1 week of training or control time Male
SA26285990423015509Training 1 week of training or control time Male
SA26286090587015509Training 2 weeks of training Female
SA26286190576015509Training 2 weeks of training Female
SA26286290578015509Training 2 weeks of training Female
SA26286390581015509Training 2 weeks of training Female
SA26286490585015509Training 2 weeks of training Female
SA26286590449015509Training 2 weeks of training Male
SA26286690450015509Training 2 weeks of training Male
SA26286790444015509Training 2 weeks of training Male
SA26286890441015509Training 2 weeks of training Male
SA26286990439015509Training 2 weeks of training Male
SA26287090412015509Training 4 weeks of training Female
SA26287190420015509Training 4 weeks of training Female
SA26287290406015509Training 4 weeks of training Female
SA26287390410015509Training 4 weeks of training Female
SA26287490416015509Training 4 weeks of training Female
SA26287590280015509Training 4 weeks of training Male
SA26287690281015509Training 4 weeks of training Male
SA26287790292015509Training 4 weeks of training Male
SA26287890289015509Training 4 weeks of training Male
SA26287990283015509Training 4 weeks of training Male
SA26288090267015509Training 8 weeks of training or control time Female
SA26288190259015509Training 8 weeks of training or control time Female
SA26288290251015509Training 8 weeks of training or control time Female
SA26288390254015509Training 8 weeks of training or control time Female
SA26288490258015509Training 8 weeks of training or control time Female
SA26288590218015509Training 8 weeks of training or control time Male
SA26288690222015509Training 8 weeks of training or control time Male
SA26288790227015509Training 8 weeks of training or control time Male
SA26288890225015509Training 8 weeks of training or control time Male
SA26288990223015509Training 8 weeks of training or control time Male
Showing results 1 to 88 of 88

Collection:

Collection ID:CO002738
Collection Summary:-
Sample Type:Gastrocnemius

Treatment:

Treatment ID:TR002754
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002751
Sampleprep Summary:See SOP
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004315
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity II
Column Agilent ZORBAX RRHD Extend C18 (150 x 2.1mm, 1.8µm,80A,1200 bar)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode NEGATIVE
Units peak area

Chromatography:

Chromatography ID:CH003221
Methods Filename:pass1b_rpneg_rppos_ionpneg_methods.pdf
Instrument Name:Agilent 1290 Infinity II
Column Name:Agilent ZORBAX RRHD Extend C18 (150 x 2.1mm, 1.8µm,80A,1200 bar)
Column Temperature:35C
Flow Gradient:t=0 min: 100%/0% (%A/%B) @ 0.25 ml/min | t=2 min: 100%/0% (%A/%B) @ 0.25 ml/min | t=12 min: 1%/99% (%A/%B) @ 0.25 ml/min | t=18 min: 1%/99% (%A/%B) @ 0.25 ml/min | t=28 min: 100%/0% (%A/%B) @ 0.40 ml/min | t=29.9 min: 100%/0% (%A/%B) @ 0.40 ml/min | t=30 min: 100%/0% (%A/%B) @ 0.25 ml/min
Flow Rate:0.25 ml/min; 0.4 ml/min
Solvent A:97% water/3% methanol; 15 mM tributylamine; 10 mM acetic
Solvent B:100% methanol; 15 mM tributylamine; 10 mM acetic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004062
Analysis ID:AN004315
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:See SOP protocol MiCAS_SOP004_Data post-processing and QC_v2_20190904.pdf
Ion Mode:NEGATIVE
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