Summary of Study ST002645

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002645
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Gastrocnemius - Untargeted Lipidomics, Reversed-Phase Positive
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Georgia Institute of Technology
DepartmentDepartment of Chemistry and Biochemistry
LaboratorySystems Mass Spectrometry Core (SyMSC)
Last NameFacundo
First NameFernandez
Address901 Atlantic Dr NW, Atlanta, GA 30332-0400
Emailfacundo.fernandez@chemistry.gatech.edu
Phone(404) 385-4432
Submit Date2023-05-01
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Fernandez Facundo Fernandez Facundo
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002747
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26295890252015510Control 8 weeks of training or control time Female
SA26295990265015510Control 8 weeks of training or control time Female
SA26296090266015510Control 8 weeks of training or control time Female
SA26296190248015510Control 8 weeks of training or control time Female
SA26296290245015510Control 8 weeks of training or control time Female
SA26296390229015510Control 8 weeks of training or control time Male
SA26296490217015510Control 8 weeks of training or control time Male
SA26296590239015510Control 8 weeks of training or control time Male
SA26296690232015510Control 8 weeks of training or control time Male
SA26296790237015510Control 8 weeks of training or control time Male
SA262968SampleBlank05QC-Blank 0 hour -
SA262969SampleBlank06QC-Blank 0 hour -
SA262970SampleBlank01QC-Blank 0 hour -
SA262971SampleBlank04QC-Blank 0 hour -
SA262972SampleBlank02QC-Blank 0 hour -
SA262973SampleBlank03QC-Blank 0 hour -
SA262974QC03QC-Pooled 0 hour -
SA262975QC02QC-Pooled 0 hour -
SA262976QC04QC-Pooled 0 hour -
SA262977QC01QC-Pooled 0 hour -
SA262978QC07QC-Pooled 0 hour -
SA262979QC08QC-Pooled 0 hour -
SA262980QC06QC-Pooled 0 hour -
SA262981QC05QC-Pooled 0 hour -
SA262982IPE_Reference_GastrocnemiusQC-Reference 0 hour -
SA262983IPE_Reference_Gastrocnemius02QC-Reference 0 hour -
SA262984SED_Reference_GastrocnemiusQC-Reference 0 hour -
SA262985SED_Reference_Gastrocnemius02QC-Reference 0 hour -
SA26298690560015510Training 1 week of training or control time Female
SA26298790559015510Training 1 week of training or control time Female
SA26298890567015510Training 1 week of training or control time Female
SA26298990571015510Training 1 week of training or control time Female
SA26299090564015510Training 1 week of training or control time Female
SA26299190430015510Training 1 week of training or control time Male
SA26299290423015510Training 1 week of training or control time Male
SA26299390421015510Training 1 week of training or control time Male
SA26299490426015510Training 1 week of training or control time Male
SA26299590422015510Training 1 week of training or control time Male
SA26299690578015510Training 2 weeks of training Female
SA26299790581015510Training 2 weeks of training Female
SA26299890585015510Training 2 weeks of training Female
SA26299990587015510Training 2 weeks of training Female
SA26300090576015510Training 2 weeks of training Female
SA26300190450015510Training 2 weeks of training Male
SA26300290449015510Training 2 weeks of training Male
SA26300390444015510Training 2 weeks of training Male
SA26300490439015510Training 2 weeks of training Male
SA26300590441015510Training 2 weeks of training Male
SA26300690410015510Training 4 weeks of training Female
SA26300790406015510Training 4 weeks of training Female
SA26300890412015510Training 4 weeks of training Female
SA26300990416015510Training 4 weeks of training Female
SA26301090420015510Training 4 weeks of training Female
SA26301190280015510Training 4 weeks of training Male
SA26301290281015510Training 4 weeks of training Male
SA26301390283015510Training 4 weeks of training Male
SA26301490292015510Training 4 weeks of training Male
SA26301590289015510Training 4 weeks of training Male
SA26301690254015510Training 8 weeks of training or control time Female
SA26301790251015510Training 8 weeks of training or control time Female
SA26301890259015510Training 8 weeks of training or control time Female
SA26301990258015510Training 8 weeks of training or control time Female
SA26302090267015510Training 8 weeks of training or control time Female
SA26302190222015510Training 8 weeks of training or control time Male
SA26302290218015510Training 8 weeks of training or control time Male
SA26302390225015510Training 8 weeks of training or control time Male
SA26302490227015510Training 8 weeks of training or control time Male
SA26302590223015510Training 8 weeks of training or control time Male
Showing results 1 to 68 of 68

Collection:

Collection ID:CO002740
Collection Summary:-
Sample Type:Gastrocnemius

Treatment:

Treatment ID:TR002756
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002753
Sampleprep Summary:IPA extraction with three cycles of freeze/thaw(with sonication)
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004317
Analysis type MS
Chromatography type Reversed phase
Chromatography system Vanquish horizon LC
Column Thermo Accucore C30 (100 x 2.1mm,2.6um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH003223
Chromatography Summary:global lipid separation
Methods Filename:pass1b_lrpneg_lrppos_methods.pdf
Instrument Name:Vanquish horizon LC
Column Name:Thermo Accucore C30 (100 x 2.1mm,2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=1 min: 40%/60% (%A/%B) @ 0.4 ml/min | t=5 min: 30%/70% (%A/%B) @ 0.4 ml/min | t=8 min: 10%/90% (%A/%B) @ 0.4 ml/min | t=8.2 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.5 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.7 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=12 min: 80%/20% (%A/%B) @ 0.4 ml/min
Flow Rate:0.4 ml/min
Solvent A:40% water/60% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Solvent B:10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004064
Analysis ID:AN004317
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:none
Ion Mode:POSITIVE
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