Summary of Study ST002746

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001709. The data can be accessed directly via it's Project DOI: 10.21228/M8ST56 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002746
Study TitleFibroblast growth factor-21 treatment induces skeletal muscle atrophy and increases plasma amino acids in female mice: a potential role for glucocorticoids.
Study TypePharmacological treatment of DIO mice
Study SummaryMale and female mice with FGF21 or saline, delivered either peripherally or directly to the brain, to determine its effect on skeletal muscle. To identify metabolic pathways affected by FGF21, we analyzed untargeted primary metabolites measured in plasma by GCTOF-MS
Institute
University of California, Davis
DepartmentNPB
LaboratoryKaren K. Ryan
Last NameMichael
First NameGoodson
AddressSVM APC, 1 Shields Ave, Davis, CA 95616
Emailmlgoodson@ucdavis.edu
Phone5306019969
Submit Date2023-06-26
Num Groups4
Total Subjects46
Num Males24
Num Females22
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2025-06-02
Release Version1
Goodson Michael Goodson Michael
https://dx.doi.org/10.21228/M8ST56
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001709
Project DOI:doi: 10.21228/M8ST56
Project Title:Fibroblast growth factor-21 treatment induces skeletal muscle atrophy and increases plasma amino acids in female mice: a potential role for glucocorticoids.
Project Type:Untargeted metabolomics
Project Summary:We analyzed untargeted 'primary metabolites' (carbohydrates and sugar phosphates, amino acids, hydroxyl acids, free fatty acids, purines, pyrimidines, aromatics, exposome-derived chemicals) in plasma from high fat diet-fed (60% kcal, Research Diets Cat# D12492) DIO male and female mice treated for 12 days with FGF21 (0.2mg/kg/d, i.p.) or saline (n=11-13/group).
Institute:University of California, Davis
Department:Neurobiology, Physiology and Behavior
Laboratory:Dr. Karen K. Ryan
Last Name:Goodson
First Name:Michael
Address:SVM APC, 1 Shields Ave. Davis, CA 95616
Email:mlgoodson@ucdavis.edu
Phone:530.602.9969
Funding Source:NIH NIDDK
Contributors:Karen K. Ryan, Karl Larsen, Michael L. Goodson

Subject:

Subject ID:SU002853
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Gender:Male and female
Animal Animal Supplier:Internal Breeding from JAX stock
Animal Housing:Single
Animal Light Cycle:12/12
Animal Feed:ad libidum high fat diet (60% kcal, Research Diets Cat#D12492)
Animal Water:ad libidum
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sex Treatment
SA289205170515aOEsa17_1F FGF21
SA289206170515aOEsa28_1F FGF21
SA289207170515aOEsa46_1F FGF21
SA289208170515aOEsa40_1F FGF21
SA289209170515aOEsa02_1F FGF21
SA289210170515aOEsa31_1F FGF21
SA289211170515aOEsa43_1F FGF21
SA289212170515aOEsa14_1F FGF21
SA289213170515aOEsa36_1F FGF21
SA289214170515aOEsa42_1F FGF21
SA289215170515aOEsa12_1F FGF21
SA289216170515aOEsa08_1F Saline
SA289217170515aOEsa07_1F Saline
SA289218170515aOEsa45_1F Saline
SA289219170515aOEsa26_1F Saline
SA289220170515aOEsa15_1F Saline
SA289221170515aOEsa41_1F Saline
SA289222170515aOEsa32_1F Saline
SA289223170515aOEsa21_1F Saline
SA289224170515aOEsa38_1F Saline
SA289225170515aOEsa25_1F Saline
SA289226170515aOEsa35_1F Saline
SA289227170515aOEsa01_1M FGF21
SA289228170515aOEsa30_1M FGF21
SA289229170515aOEsa13_1M FGF21
SA289230170515aOEsa23_1M FGF21
SA289231170515aOEsa06_1M FGF21
SA289232170515aOEsa39_1M FGF21
SA289233170515aOEsa44_1M FGF21
SA289234170515aOEsa16_1M FGF21
SA289235170515aOEsa05_1M FGF21
SA289236170515aOEsa22_1M FGF21
SA289237170515aOEsa33_1M FGF21
SA289238170515aOEsa29_1M FGF21
SA289239170515aOEsa18_1M Saline
SA289240170515aOEsa24_1M Saline
SA289241170515aOEsa10_1M Saline
SA289242170515aOEsa20_1M Saline
SA289243170515aOEsa27_1M Saline
SA289244170515aOEsa19_1M Saline
SA289245170515aOEsa09_1M Saline
SA289246170515aOEsa47_1M Saline
SA289247170515aOEsa37_1M Saline
SA289248170515aOEsa04_1M Saline
SA289249170515aOEsa03_1M Saline
SA289250170515aOEsa11_1M Saline
SA289251170515aOEsa34_1M Saline
Showing results 1 to 47 of 47

Collection:

Collection ID:CO002846
Collection Summary:K3EDTA Plasma was collected at necropsy.
Sample Type:Blood (plasma)
Collection Location:Trunk Blood

Treatment:

Treatment ID:TR002862
Treatment Summary:DIO mice treated for 12 days with FGF21 (0.2mg/kg/d, i.p)
Treatment Compound:human FGF21 (ProSpecBio, Rehovot, Israel)
Treatment Route:IP
Treatment Dose:0.2 mg/kg
Treatment Vehicle:Saline
Animal Endp Euthanasia:Pentobarbital injection

Sample Preparation:

Sampleprep ID:SP002859
Sampleprep Summary:Samples extracted using 1mL of 3:3:2 ACN:IPA:H2O (v/v/v). Half of the sample was dried to completeness and then derivatized using 10 uL of 40 mg/mL of Methoxyamine in pyridine. They are shaken at 30C for 1.5 hours. Then 91 uL of MSTFA + FAMEs to each sample and they are shaken at 37C for 0.5 hours to finish derivatization.

Chromatography:

Chromatography ID:CH003344
Chromatography Summary:We use a 7890A GC coupled with a LECO TOF. 0.5 uL of derivatized sample is injected using a splitless method onto a RESTEK RTX-5SIL MS column with an Intergra-Guard at 275C with a helium flow of 1 mL/min. The GC oven is set to hold at 50C for 1 min then ramp to 20C/min to 330C and then hold for 5 min. The transferline is set to 280C while the EI ion source is set to 250C. The Mass spec parameters collect data from 85m/z to 500m/z at an acquisition rate of 17 spectra/sec.
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
Column Temperature:-
Flow Gradient:N/A for GC
Flow Rate:-
Solvent A:N/A for GC
Solvent B:N/A for GC
Chromatography Type:GC
Solvent C:N/A for GC

Analysis:

Analysis ID:AN004453
Analysis Type:MS
Chromatography ID:CH003344
Num Factors:4
Num Metabolites:101
Units:Peak Height
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