Summary of Study ST002797

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001745. The data can be accessed directly via it's Project DOI: 10.21228/M8513X This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002797
Study TitleFetal metabolic adaptations to cardiovascular stress in twin-twin transfusion syndrome
Study SummaryMonochorionic-diamniotic twin pregnancies comprise 70% of identical twin pregnancies and are susceptible to unique complications arising from a single placenta shared by two fetuses. Twin-twin transfusion syndrome (TTTS) is a constellation of disturbances caused by unequal blood flow within the shared placenta giving rise to a major hemodynamic imbalance between the twins. If untreated, it leads to fetal cardiac failure and death. Here, we applied TTTS as a model to uncover fetal metabolic adaptations to cardiovascular stress. We compared untargeted mass spectrometry-based metabolomic analyses of amniotic fluid samples from a cohort of severe TTTS cases showing sonographic evidence of increased afterload and heart failure vs. uncomplicated singleton controls. Amniotic fluid metabolites demonstrated footprints of changes in fatty acid, glucose, and steroid hormone metabolism in TTTS. Among TTTS cases, unsupervised principal component analysis revealed two distinct clusters of disease defined by levels of glucose metabolites, amino acids, urea, and redox status. Our results suggest that the human fetal heart can adapt to hemodynamic stress by modulating its glucose metabolism. Furthermore, we have uncovered heterogeneity among cases of severe TTTS suggesting potential differences in the ability of individual fetuses to respond to cardiovascular stress.
Institute
University of Texas Health Science Center at Houston
Last NameParchem
First NameJacqueline
Address6431 Fannin, MSB 3.286
Emailjacqueline.g.parchem@uth.tmc.edu
Phone415-250-6257
Submit Date2023-07-01
Raw Data AvailableYes
Raw Data File Type(s)mzdata.xml
Analysis Type DetailLC-MS
Release Date2023-08-15
Release Version1
Jacqueline Parchem Jacqueline Parchem
https://dx.doi.org/10.21228/M8513X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001745
Project DOI:doi: 10.21228/M8513X
Project Title:Amniotic fluid metabolites in TTTS
Project Summary:Monochorionic-diamniotic twin pregnancies comprise 70% of identical twin pregnancies and are susceptible to unique complications arising from a single placenta shared by two fetuses. Twin-twin transfusion syndrome (TTTS) is a constellation of disturbances caused by unequal blood flow within the shared placenta giving rise to a major hemodynamic imbalance between the twins. If untreated, it leads to fetal cardiac failure and death. Here, we applied TTTS as a model to uncover fetal metabolic adaptations to cardiovascular stress. We compared untargeted mass spectrometry-based metabolomic analyses of amniotic fluid samples from a cohort of severe TTTS cases showing sonographic evidence of increased afterload and heart failure vs. uncomplicated singleton controls. Amniotic fluid metabolites demonstrated footprints of changes in fatty acid, glucose, and steroid hormone metabolism in TTTS. Among TTTS cases, unsupervised principal component analysis revealed two distinct clusters of disease defined by levels of glucose metabolites, amino acids, urea, and redox status. Our results suggest that the human fetal heart can adapt to hemodynamic stress by modulating its glucose metabolism. Furthermore, we have uncovered heterogeneity among cases of severe TTTS suggesting potential differences in the ability of individual fetuses to respond to cardiovascular stress.
Institute:The University of Texas Health Science Center at Houston
Last Name:Parchem
First Name:Jacqueline
Address:6431 Fannin, MSB 3.286, Houston, TX, 77030, USA
Email:jacqueline.g.parchem@uth.tmc.edu
Phone:415-250-6257

Subject:

Subject ID:SU002904
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA300454C1_160Control
SA300455C9_213Control
SA300456C10_215Control
SA300457C2_171Control
SA300458C7_190Control
SA300459C8_194Control
SA300460C6_190Control
SA300461C4_186Control
SA300462C3_183Control
SA300463C5_189Control
SA300464T17_281DTTTS
SA300465T16_262DTTTS
SA300466T15_258DTTTS
SA300467T14_253RTTTS
SA300468T13_231RTTTS
SA300469T22_421DTTTS
SA300470T21_360RTTTS
SA300471T20_322RTTTS
SA300472T19_317RTTTS
SA300473T18_287DTTTS
SA300474T7_121DRTTTS
SA300475T4_109RTTTS
SA300476T3_106DRTTTS
SA300477T2_52DTTTS
SA300478T1_48DTTTS
SA300479T5_114RTTTS
SA300480T6_118DRTTTS
SA300481T11_181RTTTS
SA300482T10_175RTTTS
SA300483T9_164DTTTS
SA300484T8_161RTTTS
SA300485T12_184RTTTS
Showing results 1 to 32 of 32

Collection:

Collection ID:CO002897
Collection Summary:Amniotic fluid was previously collected and banked from pregnant individuals with monochorionic-diamniotic twins complicated by TTTS undergoing fetoscopic laser ablation of placental anastomoses at the UTHealth Houston Fetal Center at Children’s Memorial Hermann Hospital. Samples were collected from the recipient twin sac immediately upon entry with the operative cannula and prior to placental laser ablation or amnioinfusion for improving the visualization. The amniotic fluid was centrifuged, and the supernatant was stored at -80 C for future use. Frozen genetic amniocentesis samples, discarded from further analyses, served as controls. For TTTS cases, demographics, clinical characteristics, and outcomes were abstracted from the Fetal Center research database which is maintained by trained research staff. For controls, available clinical variables were limited to maternal age, gestational age, indication for amniocentesis, and genetic testing results.
Sample Type:Amniotic fluid

Treatment:

Treatment ID:TR002913
Treatment Summary:not applicable

Sample Preparation:

Sampleprep ID:SP002910
Sampleprep Summary:Amniotic fluid metabolites were extracted by addition of 1 part amniotic fluid to 15 parts 70% acetonitrile in ddH2O (vol:vol). The mixture was briefly vortexed and then centrifuged for 5 min at 16,000 × g to pellet precipitated proteins. The protein pellet was solubilized in 0.2M NaOH and quantified by DC Protein Assay (Bio-Rad). The volume of metabolite extract was normalized by amniotic protein content.

Chromatography:

Chromatography ID:CH003419
Instrument Name:Agilent 6550 QTOF
Column Name:MicroSolv Diamond Hydride (150 x 2.1mm, 4um)
Column Temperature:25
Flow Gradient:0-1.0 min, 99% B; 1.0-15.0 min, to 20% B; 15.0 to 29.0, 0% B; 29.1 to 37min, 99% B
Flow Rate:0.4 mL/min
Solvent A:50% isopropanol/50% water; 0.025% acetic acid
Solvent B:90% acetonitrile/10% water; 5 mM ammonium acetate
Chromatography Type:Normal phase

Analysis:

Analysis ID:AN004550
Analysis Type:MS
Chromatography ID:CH003419
Num Factors:2
Num Metabolites:165
Rt Units:Minutes
Units:intensity
  
Analysis ID:AN004551
Analysis Type:MS
Chromatography ID:CH003419
Num Factors:2
Num Metabolites:155
Rt Units:Minutes
Units:intensity
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