Summary of Study ST002801

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001748. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ7M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002801
Study TitleInvestigation of metabolism in hypertrophic cardiomyopathy
Study SummaryWe propose the use of targeted metabolomics to define the metabolic and molecular pathways altered in 2 mouse models (R92W-TnT and R403Q-MHC) of hypertrophic cardiomyopathy (HCM) that span the spectrum of human disease (heart failure and sudden death), with the goal of identifying treatment targets. Parallel targeted metabolomics studies will be performed in heart tissue and plasma at rest and following inotrope stimulation. Since energy compromise is expected to be most marked when the heart is subject to increased workload, as is the case during high-intensity exercise or inotropic stimulation, we propose metabolomics studies in heart tissue and plasma, at rest and following inotrope stimulation. We anticipate that the results of these studies will allow us to move forward with further investigations into specific metabolites of interest as biomarkers, to be tested in HCM patients in future studies.
Institute
University of California, San Francisco
Last NameAbraham
First NameMaria Roselle
Address555 Mission Bay Boulevard South, San Francisco, CA 94158
EmailRoselle.Abraham@ucsf.edu
Phone415-502-3911
Submit Date2023-07-25
Num Groups2 groups of 2 (i.e., mutant vs control of two mouse models of HCM (R402Q-MyHC, R92W-TnT)
Total Subjects32
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2023-09-06
Release Version1
Maria Roselle Abraham Maria Roselle Abraham
https://dx.doi.org/10.21228/M8RQ7M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001748
Project DOI:doi: 10.21228/M8RQ7M
Project Title:Investigation of metabolism in hypertrophic cardiomyopathy
Project Summary:We propose the use of targeted metabolomics to define the metabolic and molecular pathways altered in 2 mouse models (R92W-TnT and R403Q-MHC) of hypertrophic cardiomyopathy (HCM) that span the spectrum of human disease (heart failure and sudden death), with the goal of identifying treatment targets. Parallel targeted metabolomics studies will be performed in heart tissue and plasma at rest and following inotrope stimulation. Since energy compromise is expected to be most marked when the heart is subject to increased workload, as is the case during high-intensity exercise or inotropic stimulation, we propose metabolomics studies in heart tissue and plasma, at rest and following inotrope stimulation. We anticipate that the results of these studies will allow us to move forward with further investigations into specific metabolites of interest as biomarkers, to be tested in HCM patients in future studies.
Institute:University of California, San Francisco
Department:Hypertrophic Cardiomyopathy Center of Excellence
Laboratory:UCSF Smith Cardiovascular Research Building
Last Name:Abraham
First Name:Maria Roselle
Address:555 Mission Bay Boulevard South, San Francisco, CA 94158
Email:Roselle.Abraham@ucsf.edu
Phone:415-502-3911
Contributors:Arpana Vaniya (avaniya@ucdavis.edu), Anja Karlstaedt (Anja.Karlstaedt@cshs.org)

Subject:

Subject ID:SU002908
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA300760M492Control MHC
SA300761M503Control MHC
SA300762M499Control MHC
SA300763M487Control MHC
SA300764M438Control MHC
SA300765M447Control MHC
SA300766M440Control MHC
SA300767M485Control MHC
SA300768T545Control TNT
SA300769T547Control TNT
SA300770T544Control TNT
SA300771T518Control TNT
SA300772T515Control TNT
SA300773T524Control TNT
SA300774T520Control TNT
SA300775T522Control TNT
SA300776M540MHC
SA300777M486MHC
SA300778M498MHC
SA300779M504MHC
SA300780M483MHC
SA300781M441MHC
SA300782M439MHC
SA300783M500MHC
SA300784T542TNT
SA300785T546TNT
SA300786T540TNT
SA300787T523TNT
SA300788T519TNT
SA300789T521TNT
SA300790T525TNT
SA300791T527TNT
Showing results 1 to 32 of 32

Collection:

Collection ID:CO002901
Collection Summary:Excision while the heart still beating and flash freeze within 15 seconds of excision
Sample Type:Heart

Treatment:

Treatment ID:TR002917
Treatment Summary:Saline IP and heparine SC injection in control & mutant group. After 10 minutes of saline injection we performed cervical dislocation, whole heart excision and flash freeze within 15 seconds of excision.

Sample Preparation:

Sampleprep ID:SP002914
Sampleprep Summary:2 mg of lyophilized tissue extracted with 1 mL 3:3:2 ACN/IPA/H2O 450 uL of extract dried and derivatized Derivatization is 10 uL of MeOX + Pyridine + 90 uL MSTFA

Combined analysis:

Analysis ID AN004557
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus IV TOF
Ion Mode POSITIVE
Units Peak Heights normalized by mTIC

Chromatography:

Chromatography ID:CH003424
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
Column Temperature:50-330°C
Flow Gradient:NA
Flow Rate:1mL/min
Solvent A:NA
Solvent B:NA
Chromatography Type:GC

MS:

MS ID:MS004304
Analysis ID:AN004557
Instrument Name:Leco Pegasus IV TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:Mass spectrometer settings: A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da.
Ion Mode:POSITIVE
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