Summary of Study ST002879
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001800. The data can be accessed directly via it's Project DOI: 10.21228/M81X5D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002879 |
| Study Title | Metabolomic profiles in P. gingivalis treated with curcumin |
| Study Summary | Curcumin, a major constituent of turmeric rhizomes (Curcuma-longa), has been reported to have various functions, including anti-oxidant, anti-inflammatory, anti-tumor, and anti-bacterial effects. Thus, curcumin is expected to effective for the prevention of periodontal disease. We previously showed that curcumin markedly inhibited the growth of P. gingivalis, and prevented biofilm formation as well as bacterial protease activity at a low concentration. Nevertheless, negligible bactericidal effects against some oral bacterial species such as Aggregatibacter actinomycetemcomitans and Streptococcus mitis, were found in order investigations. These reports suggest that curcumin exhibit species-specific antimicrobial activities, the molecular basis remains to be elucidated. This metabolomic analysis was conducted to investigate the mechanism of the antimicrobial action of curcumin toward P. gingivalis. |
| Institute | Osaka University |
| Department | Graduate School of Dentistry |
| Laboratory | Joint Research Laboratory for Advanced Oral Environmental Science (SARAYA) |
| Last Name | Murai |
| First Name | Hiroki |
| Address | 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan |
| murai-h@saraya.com | |
| Phone | +81-6-6879-2922 |
| Submit Date | 2023-09-22 |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-09-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001800 |
| Project DOI: | doi: 10.21228/M81X5D |
| Project Title: | Metabolomic profiles in P. gingivalis cells treated with curcumin |
| Project Summary: | Metabolomics data for Porphyromonas gingivalis in the presence or absence of curcumin |
| Institute: | Osaka University |
| Department: | Graduate School of Dentistry |
| Laboratory: | Joint Research Laboratory for Advanced Oral Environmental Science (SARAYA) |
| Last Name: | Murai |
| First Name: | Hiroki |
| Address: | 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan |
| Email: | murai-h@saraya.com |
| Phone: | +81-6-6879-2922 |
Subject:
| Subject ID: | SU002992 |
| Subject Type: | Bacteria |
| Subject Species: | Porphyromonas gingivalis ATCC33277 |
| Taxonomy ID: | 431947 |
| Species Group: | Bacteria |
Factors:
Subject type: Bacteria; Subject species: Porphyromonas gingivalis ATCC33277 (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA314672 | Control1 | incubated in TSB |
| SA314673 | Control3 | incubated in TSB |
| SA314674 | Control2 | incubated in TSB |
| SA314675 | C3 | incubated in TSB+Curcumin(10mg/L) |
| SA314676 | C1 | incubated in TSB+Curcumin(10mg/L) |
| SA314677 | C2 | incubated in TSB+Curcumin(10mg/L) |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO002985 |
| Collection Summary: | Incubated cells were collected by centrifugation at 4℃. Collected cells were washed with Milli-Q water and re-collected by centrifugation at 4℃. Bacterial pellets (>5E+09 cells) were immediately fixed by methanol containing 5 µM internal standard. |
| Sample Type: | Bacterial cells |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003001 |
| Treatment Summary: | P. gingivalis cells were incubated anaerobically with TSB(DMSO0.1%) in the presence or absence of 1mg/L of curcumin at 37℃ for 24h |
Sample Preparation:
| Sampleprep ID: | SP002998 |
| Sampleprep Summary: | Samples were stirred with 2000μL chloroform and 800 μL of Milli-Q water, and centrifuged at 4℃. After centrifugation, the aqueous layer is filtered by ultrafiltration tube. Filtrate samples were dried and dissolved in 25μL Milli-Q water. |
Chromatography:
| Chromatography ID: | CH003552 |
| Instrument Name: | Agilent CE |
| Column Name: | Agilent Fused silica capillary (80cm x 50um) |
| Column Temperature: | none |
| Flow Gradient: | none |
| Flow Rate: | none |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | CE |
Analysis:
| Analysis ID: | AN004717 |
| Analysis Type: | MS |
| Chromatography ID: | CH003552 |
| Num Factors: | 2 |
| Num Metabolites: | 162 |
| Units: | Relative area |
| Analysis ID: | AN004718 |
| Analysis Type: | MS |
| Chromatography ID: | CH003552 |
| Num Factors: | 2 |
| Num Metabolites: | 105 |
| Units: | Relative area |
