Summary of Study ST002931

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001823. The data can be accessed directly via it's Project DOI: 10.21228/M82Q61 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002931
Study TitleAnalyzing Metabolic Alterations in the Gut, Blood, and Brain of Mice Induced by Running Exercise Through Gas Chromatography Mass Spectrometry.
Study SummaryStudying the metabolic impact of running exercise on the gut, blood, and specific brain regions like the hippocampus and brainstem is crucial for comprehending the broader health effects of physical activity. In our six-week study, we utilized a mouse model (C57BL/6 genotype) to investigate these metabolic changes. Employing gas chromatography coupled with mass spectrometry followed by metabolomics for a comprehensive analysis, our approach offers insights into how exercise influences metabolic processes, including brain function. Our findings hold the potential to shape more effective exercise strategies for enhancing overall health and cognitive function.
Institute
University of Puerto Rico, School of Medicine
DepartmentBiochemistry
Last NameChorna
First NameNataliya
AddressUniversity of Puerto Rico, Medical Sciences Campus, San Juan, PR 00935
Emailnataliya.chorna@upr.edu
Phone787-758-2525 x 1640
Submit Date2023-10-14
Num Groups2
Total Subjects24
Num Males24
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2023-10-30
Release Version1
Nataliya Chorna Nataliya Chorna
https://dx.doi.org/10.21228/M82Q61
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001823
Project DOI:doi: 10.21228/M82Q61
Project Title:Analyzing Metabolic Alterations in the Gut, Blood, and Brain of Mice Induced by Running Exercise Through Gas Chromatography Mass Spectrometry
Project Summary:Studying the metabolic impact of running exercise on the gut, blood, and specific brain regions like the hippocampus and brainstem is crucial for comprehending the broader health effects of physical activity. In our six-week study, we utilized a mouse model (C57BL/6 genotype) to investigate these metabolic changes. Employing gas and liquid chromatography coupled with mass spectrometry followed by metabolomics for a comprehensive analysis, our approach offers insights into how exercise influences metabolic processes, including brain function. Our findings hold the potential to shape more effective exercise strategies for enhancing overall health and cognitive function.
Institute:University of Puerto Rico, School of Medicine
Department:Biochemistry
Last Name:Chorna
First Name:Nataliya
Address:University of Puerto Rico, Medical Sciences Campus, San Juan, PR 00935
Email:nataliya.chorna@upr.edu
Phone:7877582525 ext 1640
Funding Source:NIGMS-NIH-PRINBRE-P20GM103475

Subject:

Subject ID:SU003044
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:20 wks
Gender:Male
Animal Animal Supplier:Jackson Lab

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample Treatment
SA318126bd_r3blood Running
SA318127bd_r8blood Running
SA318128bd_r9blood Running
SA318129bd_r4blood Running
SA318130bd_r2blood Running
SA318131bd_r1blood Running
SA318132bd_r7blood Running
SA318133bd_r6blood Running
SA318134bd_r5blood Running
SA318135bd_s9blood Sedentary
SA318136bd_s8blood Sedentary
SA318137bd_s3blood Sedentary
SA318138bd_s1blood Sedentary
SA318139bd_s2blood Sedentary
SA318140bd_s7blood Sedentary
SA318141bd_s6blood Sedentary
SA318142bd_s4blood Sedentary
SA318143bd_s5blood Sedentary
SA318144bs_r6brainstem Running
SA318145bs_r5brainstem Running
SA318146bs_r3brainstem Running
SA318147bs_r2brainstem Running
SA318148bs_r7brainstem Running
SA318149bs_r4brainstem Running
SA318150bs_r9brainstem Running
SA318151bs_r12brainstem Running
SA318152bs_r1brainstem Running
SA318153bs_r11brainstem Running
SA318154bs_r10brainstem Running
SA318155bs_r8brainstem Running
SA318156bs_s10brainstem Sedentary
SA318157bs_s4brainstem Sedentary
SA318158bs_s3brainstem Sedentary
SA318159bs_s2brainstem Sedentary
SA318160bs_s1brainstem Sedentary
SA318161bs_s5brainstem Sedentary
SA318162bs_s6brainstem Sedentary
SA318163bs_s11brainstem Sedentary
SA318164bs_s9brainstem Sedentary
SA318165bs_s8brainstem Sedentary
SA318166bs_s7brainstem Sedentary
SA318167bs_s12brainstem Sedentary
SA318168fec_r4feces Running
SA318169fec_r5feces Running
SA318170fec_r3feces Running
SA318171fec_r2feces Running
SA318172fec_r1feces Running
SA318173fec_r6feces Running
SA318174fec_r7feces Running
SA318175fec_r11feces Running
SA318176fec_r10feces Running
SA318177fec_r9feces Running
SA318178fec_r8feces Running
SA318179fec_r12feces Running
SA318180fec_s10feces Sedentary
SA318181fec_s7feces Sedentary
SA318182fec_s9feces Sedentary
SA318183fec_s11feces Sedentary
SA318184fec_s12feces Sedentary
SA318185fec_s6feces Sedentary
SA318186fec_s8feces Sedentary
SA318187fec_s2feces Sedentary
SA318188fec_s5feces Sedentary
SA318189fec_s3feces Sedentary
SA318190fec_s1feces Sedentary
SA318191fec_s4feces Sedentary
SA318192hp_r4hippocampus Running
SA318193hp_r5hippocampus Running
SA318194hp_r3hippocampus Running
SA318195hp_r2hippocampus Running
SA318196hp_r1hippocampus Running
SA318197hp_r6hippocampus Running
SA318198hp_r7hippocampus Running
SA318199hp_r9hippocampus Running
SA318200hp_r11hippocampus Running
SA318201hp_r12hippocampus Running
SA318202hp_r10hippocampus Running
SA318203hp_r8hippocampus Running
SA318204hp_s3hippocampus Sedentary
SA318205hp_s1hippocampus Sedentary
SA318206hp_s4hippocampus Sedentary
SA318207hp_s2hippocampus Sedentary
SA318208hp_s6hippocampus Sedentary
SA318209hp_s11hippocampus Sedentary
SA318210hp_s12hippocampus Sedentary
SA318211hp_s10hippocampus Sedentary
SA318212hp_s9hippocampus Sedentary
SA318213hp_s7hippocampus Sedentary
SA318214hp_s8hippocampus Sedentary
SA318215hp_s5hippocampus Sedentary
Showing results 1 to 90 of 90

Collection:

Collection ID:CO003037
Collection Summary:Mice were randomized into two groups, sedentary control and running experimental. Mice were housed with free access to a wireless running wheel (Med Associates). For the sedentary group, the wheels were locked in the experiment. After 42 days, blood (130 uL), feces, hippocampus and brainstem were collected and stored at -80°C.
Sample Type:blood, feces, hippocampus, brainstem
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003053
Treatment Summary:To evaluate our hypothesis that running exercise reshapes gut microbiota diversity that balances TRP metabolism in the gut, we used an established model of voluntary running exercise in 20-week-old male mice (C57BL/6J, Jackson Lab) housed individually in a standard cage in temperature-controlled (21°C) quarters with a 12-h light/12-h dark cycle. Animals were given water and food (Purina Chow) ad libitum as previously described (Chorna et al., 2013). Briefly, mice were randomized into two groups, sedentary control (n=12) and running experimental (n=12), housed with free access to a wireless running wheel (Med Associates) for six weeks. For the sedentary group, the wheels were locked in the experiment. Therefore, this group of mice could not perform running exercises. Exercise activities of the running group were recorded for each animal for the investigation to ensure that each mouse was physically active. The recording was conducted using an automatic counter and Med Associates software.

Sample Preparation:

Sampleprep ID:SP003050
Sampleprep Summary:Metabolites were extracted from feces, blood, brainstem and hippocampus. See Sample_preparation.pdf.
Sampleprep Protocol Filename:Sample_preparation.pdf
Processing Storage Conditions:Described in summary
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN004809
Analysis type MS
Chromatography type GC
Chromatography system Shimadzu GCMS/MS-TQ8050
Column Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type Triple quadrupole
MS instrument name Shimadzu GCMS/MS-TQ8050
Ion Mode POSITIVE
Units intensity

Chromatography:

Chromatography ID:CH003634
Chromatography Summary:1 μL of each sample was injected in the GC-MS. Injection mode – split (15:1). The injection port temperature was set at 280°C, and helium was used as the carrier gas at a constant linear velocity of 39 cm/sec. Metabolites were separated using a GC temperature ramping program. The GC oven was programmed from 100°C to 280°C at a rate of 4°C/min.
Methods Filename:Chromatography.pdf
Instrument Name:Shimadzu GCMS/MS-TQ8050
Column Name:Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um)
Column Temperature:280°C
Flow Gradient:NA
Flow Rate:4°C/min
Solvent A:NA
Solvent B:NA
Chromatography Type:GC

MS:

MS ID:MS004555
Analysis ID:AN004809
Instrument Name:Shimadzu GCMS/MS-TQ8050
Instrument Type:Triple quadrupole
MS Type:EI
MS Comments:MS acquisition: Metabolites were detected by setting the ion source filament energy to 70 eV, the ion source temperature - 200°C, and the scan range (mass-to-charge (m/z) 35 – 600 Da. Software/procedures used for feature assignments: Labsolution Shimadzu
Ion Mode:POSITIVE
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