Summary of Study ST002952

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001835. The data can be accessed directly via it's Project DOI: 10.21228/M8HT5Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002952
Study TitleInvestigate the impact of feeding time on the hexosamine biosynthetic pathway (HBP) in the mouse liver and heart using targeted metabolomics: primary metabolism
Study SummaryThe overall goal of this project is to advance our understanding of post-translational mechanisms that mediate metabolic regulation of time-of-day-specific protein functions to orchestrate daily rhythms and maintain homeostasis in animals. Robust daily biological rhythms over the 24-hour (h) day-night cycles are key hallmarks of animal health span and are strongly regulated by circadian clocks. Circadian clocks are cell autonomous molecular timers present in the brain and in peripheral organs that enable animals to adapt to predictable daily changes in environment and regulate rhythmic processes such as sleep-wake cycles, feeding-fasting cycles, metabolism, hormonal signaling and neuronal excitability. Besides light, the dominant time cue for the brain clock, metabolic signals from clock-controlled feeding-fasting cycles represent the most potent time cue to entrain and synchronize peripheral clocks in key organs. Much effort has been dedicated to understanding the metabolic regulation of daily biological rhythms, but many important mechanisms are only just emerging. We recently established that metabolic signals from feeding-fasting cycles regulate daily biological rhythms in Drosophila through rhythmic O-linked-N-acetylglucosaminylation (O-GlcNAcylation). Protein O-GlcNAcylation is a nutrient sensitive posttranslational modification (PTM) that is tightly linked to metabolic status, as UDP-GlcNAc, the substrate of O-GlcNAcylation, is produced from hexosamine biosynthetic pathway (HBP), which integrates the metabolites from glucose, amino acid, lipid and nucleotide metabolism. We now propose to investigate whether feeding activity can regulate daily O-GlcNAcylation rhythm in mouse liver and heart and whether the levels of HBP metabolites in mouse liver and heart are affected by different feeding time within a day/night cycle. Here, we restricted the feeding time of C57BL/6 male mice to ZT12-24 (RF12-24. ZT, zeitgeber time; ZT0 indicates light on, while ZT12 indicates light off) v.s. ZT0-12 (RF0-12) for 3 weeks and collected liver and heart tissues every 4 hours over a 24-hour period. The liver and heart samples were subjected to targeted metabolomic analysis for HBP metabolites.
Institute
University of California, Davis
DepartmentDepartment of Entomology and Nematology
LaboratoryChiu lab
Last NameChiu
First NameJoanna
Address6352 Storer Hall, One Shields Avenue, Davis, CA 95616, USA
Emailjcchiu@ucdavis.edu
Phone(530) 752-1643
Submit Date2023-10-31
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2024-04-02
Release Version1
Joanna Chiu Joanna Chiu
https://dx.doi.org/10.21228/M8HT5Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001835
Project DOI:doi: 10.21228/M8HT5Q
Project Title:Investigate the impact of feeding time on the hexosamine biosynthetic pathway (HBP) in the mouse liver and heart using targeted metabolomics
Project Summary:The overall goal of this project is to advance our understanding of post-translational mechanisms that mediate metabolic regulation of time-of-day-specific protein functions to orchestrate daily rhythms and maintain homeostasis in animals. Robust daily biological rhythms over the 24-hour (h) day-night cycles are key hallmarks of animal health span and are strongly regulated by circadian clocks. Circadian clocks are cell autonomous molecular timers present in the brain and in peripheral organs that enable animals to adapt to predictable daily changes in environment and regulate rhythmic processes such as sleep-wake cycles, feeding-fasting cycles, metabolism, hormonal signaling and neuronal excitability. Besides light, the dominant time cue for the brain clock, metabolic signals from clock-controlled feeding-fasting cycles represent the most potent time cue to entrain and synchronize peripheral clocks in key organs. Much effort has been dedicated to understanding the metabolic regulation of daily biological rhythms, but many important mechanisms are only just emerging. We recently established that metabolic signals from feeding-fasting cycles regulate daily biological rhythms in Drosophila through rhythmic O-linked-N-acetylglucosaminylation (O-GlcNAcylation). Protein O-GlcNAcylation is a nutrient sensitive posttranslational modification (PTM) that is tightly linked to metabolic status, as UDP-GlcNAc, the substrate of O-GlcNAcylation, is produced from hexosamine biosynthetic pathway (HBP), which integrates the metabolites from glucose, amino acid, lipid and nucleotide metabolism. We now propose to investigate whether feeding activity can regulate daily O-GlcNAcylation rhythm in mouse liver and heart and whether the levels of HBP metabolites in mouse liver and heart are affected by different feeding time within a day/night cycle. Here, we restricted the feeding time of C57BL/6 male mice to ZT12-24 (RF12-24. ZT, zeitgeber time; ZT0 indicates light on, while ZT12 indicates light off) v.s. ZT0-12 (RF0-12) for 3 weeks and collected liver and heart tissues every 4 hours over a 24-hour period. The liver and heart samples were subjected to targeted metabolomic analysis for HBP metabolites.
Institute:University of California, Davis
Department:Department of Entomology and Nematology
Laboratory:Chiu lab
Last Name:Chiu
First Name:Joanna
Address:6352 Storer Hall, One Shields Avenue, Davis, CA 95616, USA
Email:jcchiu@ucdavis.edu
Phone:(530) 752-1643

Subject:

Subject ID:SU003065
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id organ Treatment
SA321748Heart ZT3-rep3_039Heart Control
SA321749Heart ZT3-rep1_037Heart Control
SA321750Heart ZT23-rep3_054Heart Control
SA321751Heart ZT7-rep1_040Heart Control
SA321752Heart ZT7-rep2_041Heart Control
SA321753Heart ZT11-rep1_043Heart Control
SA321754Heart ZT7-rep3_042Heart Control
SA321755Heart ZT23-rep2_053Heart Control
SA321756Heart ZT3-rep2_038Heart Control
SA321757Heart ZT15-rep2_047Heart Control
SA321758Heart ZT15-rep1_046Heart Control
SA321759Heart ZT23-rep1_052Heart Control
SA321760Heart ZT11-rep2_044Heart Control
SA321761Heart ZT15-rep3_048Heart Control
SA321762Heart ZT11-rep3_045Heart Control
SA321763Heart ZT19-rep1_049Heart Control
SA321764Heart ZT19-rep2_050Heart Control
SA321765Heart ZT19-rep3_051Heart Control
SA321766Heart ZT3-rep2_056Heart Expt
SA321767Heart ZT23-rep3_072Heart Expt
SA321768Heart ZT3-rep1_055Heart Expt
SA321769Heart ZT3-rep3_057Heart Expt
SA321770Heart ZT7-rep2_059Heart Expt
SA321771Heart ZT23-rep2_071Heart Expt
SA321772Heart ZT7-rep3_060Heart Expt
SA321773Heart ZT7-rep1_058Heart Expt
SA321774Heart ZT15-rep3_066Heart Expt
SA321775Heart ZT15-rep1_064Heart Expt
SA321776Heart ZT11rep3_063Heart Expt
SA321777Heart ZT11-rep2_062Heart Expt
SA321778Heart ZT15-rep2_065Heart Expt
SA321779Heart ZT11-rep1_061Heart Expt
SA321780Heart ZT19-rep3_069Heart Expt
SA321781Heart ZT19-rep2_068Heart Expt
SA321782Heart ZT19-rep1_067Heart Expt
SA321783Heart ZT23-rep1_070Heart Expt
SA321784Pool_001-HHeart Heart Pool QC
SA321785Pool_003-HHeart Heart Pool QC
SA321786Pool_002-HHeart Heart Pool QC
SA321787Liver ZT3-rep2_002Liver Control
SA321788Liver ZT3-rep1_001Liver Control
SA321789Liver ZT3-rep3_003Liver Control
SA321790Liver ZT7-rep2_005Liver Control
SA321791Liver ZT23-rep3_018Liver Control
SA321792Liver ZT7-rep3_006Liver Control
SA321793Liver ZT7-rep1_004Liver Control
SA321794Liver ZT15-rep1_010Liver Control
SA321795Liver ZT11-rep1_007Liver Control
SA321796Liver ZT11-rep2_008Liver Control
SA321797Liver ZT15-rep2_011Liver Control
SA321798Liver ZT23-rep2_017Liver Control
SA321799Liver ZT15-rep3_012Liver Control
SA321800Liver ZT11-rep3_009Liver Control
SA321801Liver ZT19-rep1_013Liver Control
SA321802Liver ZT23-rep1_016Liver Control
SA321803Liver ZT19-rep3_015Liver Control
SA321804Liver ZT19-rep2_014Liver Control
SA321805Liver ZT3-rep2_020Liver Expt
SA321806Liver ZT3-rep1_019Liver Expt
SA321807Liver ZT3-rep3_021Liver Expt
SA321808Liver ZT7-rep2_023Liver Expt
SA321809Liver ZT23-rep3_036Liver Expt
SA321810Liver ZT7-rep3_024Liver Expt
SA321811Liver ZT7-rep1_022Liver Expt
SA321812Liver ZT23-rep1_034Liver Expt
SA321813Liver ZT23-rep2_035Liver Expt
SA321814Liver ZT11-rep3_027Liver Expt
SA321815Liver ZT11-rep2_026Liver Expt
SA321816Liver ZT11-rep1_025Liver Expt
SA321817Liver ZT15-rep2_029Liver Expt
SA321818Liver ZT15-rep1_028Liver Expt
SA321819Liver ZT19-rep3_033Liver Expt
SA321820Liver ZT15-rep3_030Liver Expt
SA321821Liver ZT19-rep2_032Liver Expt
SA321822Liver ZT19-rep1_031Liver Expt
SA321823Pool_006-LLiver Liver Pool QC
SA321824Pool_001-LLiver Liver Pool QC
SA321825Pool_005-LLiver Liver Pool QC
Showing results 1 to 78 of 78

Collection:

Collection ID:CO003058
Collection Summary:Mice were anesthetized using isoflurane. The liver or heart are dissected out and rinsed with PBS. Then samples were flash frozen in liquid nitrogen and stored at -80C. Samples were weight on dry ice before sending out for metabolomics analysis.
Sample Type:Heart and Liver

Treatment:

Treatment ID:TR003074
Treatment Summary:The mice were either fed at natural feeding time (during nighttime, Control group) or at unnatural feeding time (during daytime, Expt group).

Sample Preparation:

Sampleprep ID:SP003071
Sampleprep Summary:SOP -GCMS-02252019

Combined analysis:

Analysis ID AN004848 AN004849
Analysis type MS MS
Chromatography type GC GC
Chromatography system Agilent 7890 Agilent 7890
Column Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um) Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
MS Type EI EI
MS instrument type GC-TOF GC-TOF
MS instrument name Leco Pegasus IV TOF Leco Pegasus IV TOF
Ion Mode POSITIVE POSITIVE
Units peak heights ng/mg

Chromatography:

Chromatography ID:CH003661
Instrument Name:Agilent 7890
Column Name:Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
Column Temperature:NA
Flow Gradient:NA
Flow Rate:1 mL/min
Solvent A:NA
Solvent B:NA
Chromatography Type:GC

MS:

MS ID:MS004594
Analysis ID:AN004848
Instrument Name:Leco Pegasus IV TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:Mass spectrometer settings: A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da.
Ion Mode:POSITIVE
  
MS ID:MS004595
Analysis ID:AN004849
Instrument Name:Leco Pegasus IV TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:Mass spectrometer settings: A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da.
Ion Mode:POSITIVE
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