Summary of Study ST003095
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003095 |
| Study Title | Lactiplantibacillus plantarum intervention on serum choline derivatives of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of serum choline derivatives of germ-free mice. L. plantarum during gestation of humanized GF mice showed no significant differences on the serum choline derivatives. |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai distric |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001923 |
| Project DOI: | doi: 10.21228/M85149 |
| Project Title: | Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice |
| Project Summary: | The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism. |
| Institute: | Beijing Sanyuan Foods Co. Ltd |
| Last Name: | Lijun |
| First Name: | Chen |
| Address: | 8th yingchang street, yinghai distric |
| Email: | chenlijun@sanyuan.com.cn |
| Phone: | 01056306594 |
Subject:
| Subject ID: | SU003210 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA333311 | P220801156 | control |
| SA333312 | P220801159 | control |
| SA333313 | P220801157 | control |
| SA333314 | P220801158 | control |
| SA333315 | P220801165 | intervention |
| SA333316 | P220801164 | intervention |
| SA333317 | P220801161 | intervention |
| SA333318 | P220801160 | intervention |
| SA333319 | P220801162 | intervention |
| SA333320 | P220801163 | intervention |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003203 |
| Collection Summary: | Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003219 |
| Treatment Summary: | Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003216 |
| Sampleprep Summary: | Samples were added in a 2 mL centrifuge tube, add 10 μL of internal standard solution, then add 750 μL of 1% formic acid-acetonitrile solution, vortex, centrifuge for 10 min at 12,000 rpm at 4^oC, and then resovle and filter the supernatant by 0.22 μm membrane for LC-MS analysis. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Chromatography:
| Chromatography ID: | CH003825 |
| Instrument Name: | AB SCIEX Jasper HPLC |
| Column Name: | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0~1 min, 80% B; 1~2 min, 80~70% B; 2~2.5 min, 70% B; 2.5~3 min, 70~50% B; 3~3.5 min, 50% B; 3.5~4 min, 50~80% B; 4~6 min, 80% B |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 10 mM ammonium formate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | LC |
Analysis:
| Analysis ID: | AN005066 |
| Analysis Type: | MS |
| Chromatography ID: | CH003825 |
| Num Factors: | 2 |
| Num Metabolites: | 5 |
| Units: | μmol/L |
