Summary of Study ST003099
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003099 |
| Study Title | Lactiplantibacillus plantarum intervention on fecal short-chain fatty acids of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of fecal short-chain fatty acids of germ-free mice. L. plantarum during gestation of humanized GF mice showed that all fecal SCFAs showed no significant difference. |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai distric |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001923 |
| Project DOI: | doi: 10.21228/M85149 |
| Project Title: | Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice |
| Project Summary: | The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism. |
| Institute: | Beijing Sanyuan Foods Co. Ltd |
| Last Name: | Lijun |
| First Name: | Chen |
| Address: | 8th yingchang street, yinghai distric |
| Email: | chenlijun@sanyuan.com.cn |
| Phone: | 01056306594 |
Subject:
| Subject ID: | SU003214 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA333351 | 188S14 | control |
| SA333352 | 188S18 | control |
| SA333353 | 188S7 | control |
| SA333354 | 188S1 | control |
| SA333355 | 185S7 | control |
| SA333356 | 185S14 | control |
| SA333357 | 185S18 | control |
| SA333358 | 908S0 | control |
| SA333359 | 188S0 | control |
| SA333360 | 908S14 | control |
| SA333361 | 909S14 | control |
| SA333362 | 909S18 | control |
| SA333363 | 909S7 | control |
| SA333364 | 909S1 | control |
| SA333365 | 909S0 | control |
| SA333366 | 185S1 | control |
| SA333367 | 908S18 | control |
| SA333368 | 908S7 | control |
| SA333369 | 908S1 | control |
| SA333370 | 185S0 | control |
| SA333371 | 120S0 | intervention |
| SA333372 | 107S7 | intervention |
| SA333373 | 120S1 | intervention |
| SA333374 | 907S7 | intervention |
| SA333375 | 907S14 | intervention |
| SA333376 | 907S18 | intervention |
| SA333377 | 107S18 | intervention |
| SA333378 | 107S14 | intervention |
| SA333379 | 081S14 | intervention |
| SA333380 | 081S1 | intervention |
| SA333381 | 081S18 | intervention |
| SA333382 | 081S7 | intervention |
| SA333383 | 107S1 | intervention |
| SA333384 | 107S0 | intervention |
| SA333385 | 907S1 | intervention |
| SA333386 | 907S0 | intervention |
| SA333387 | 180S0 | intervention |
| SA333388 | 122S7 | intervention |
| SA333389 | 081S0 | intervention |
| SA333390 | 180S7 | intervention |
| SA333391 | 180S14 | intervention |
| SA333392 | 180S18 | intervention |
| SA333393 | 122S18 | intervention |
| SA333394 | 122S14 | intervention |
| SA333395 | 120S18 | intervention |
| SA333396 | 120S14 | intervention |
| SA333397 | 120S7 | intervention |
| SA333398 | 122S0 | intervention |
| SA333399 | 122S1 | intervention |
| SA333400 | 180S1 | intervention |
| Showing results 1 to 50 of 50 |
Collection:
| Collection ID: | CO003207 |
| Collection Summary: | Feces were collected at and stored at -80°C for SCFAs analysis. |
| Sample Type: | Feces |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003223 |
| Treatment Summary: | mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003220 |
| Sampleprep Summary: | 100 mg of sample was extracted in 50 μL of 15% phosphoric acid with 100 μL of 125 μg/mL 4-methyl valeric acid solution as internal standard and 400 μL ether, and were centrifuged at 4 °C for 10 min at 12000 rpm after vortexing for 1 min and the supernatant was transferred into the vial. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Chromatography:
| Chromatography ID: | CH003829 |
| Instrument Name: | Thermo Trace 1310 |
| Column Name: | Agilent HP-INNOWAX (30 m × 0.25 mm ID × 0.25 μm) |
| Column Temperature: | The column temperature was programmed to increase from an initial temperature of 90°C, followed by an increase to 120 °C at 10 °C/min, and to 150 °C at 5 °C/min, and finally to 250 °C at 25 °C/min which was maintained for 2 min |
| Flow Gradient: | none |
| Flow Rate: | 1 mL/min |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | GC |
Analysis:
| Analysis ID: | AN005070 |
| Analysis Type: | MS |
| Chromatography ID: | CH003829 |
| Num Factors: | 2 |
| Num Metabolites: | 7 |
| Units: | μg/g |
