Summary of Study ST003155

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001961. The data can be accessed directly via it's Project DOI: 10.21228/M87Q8B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003155
Study TitleNMR-based metabolomics combined with metabolic pathway analysis reveals metabolic heterogeneity of colorectal cancer tissue at different anatomical locations and stages
Study SummaryColorectal cancer (CRC) still remains the leading cause of cancer death worldwide. This study aimed to profile the metabolic differences of colorectal cancer tissues (CCT) at different stages and sites, as compared with their adjacent noncancerous tissues (ANT), to investigate the temporal and spatial heterogeneities of metabolic characterization. Our NMR-based metabolomics fingerprinting revealed that many of the metabolite levels were significantly altered in CCT compared to ANT and esophageal cancer tissues, indicating deregulations of glucose metabolism, one-carbon metabolism, glutamine metabolism, amino acid metabolism, fatty acid metabolism, TCA cycle, choline metabolism, etc. A total of five biomarker metabolites, including glucose, glutamate, alanine, valine and histidine, were identified to distinguish between early and advanced stages of CCT. Metabolites that distinguish the different anatomical sites of CCT include glucose, glycerol, glutamine, inositol, succinate, and citrate. Those significant metabolic differences in CRC tissues at different pathological stages and sites suggested temporal and spatial heterogeneities of metabolic characterization in CCT, providing a metabolic foundation for further study on biofluid metabolism in CRC early detection.
Institute
Shantou University Medical College
DepartmentRadiology Department, Second Affiliated Hospital
Last NameLin
First NameYan
AddressNo. 69, Dongxia North Road, Shantou, Guangdong, China, Shantou, Guangdong, China, 515041, China
Email994809889@qq.com
Phone+86 18823992148
Submit Date2024-03-28
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2024-05-22
Release Version1
Yan Lin Yan Lin
https://dx.doi.org/10.21228/M87Q8B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001961
Project DOI:doi: 10.21228/M87Q8B
Project Title:NMR-based metabolomics combined with metabolic pathway analysis reveals metabolic heterogeneity of colorectal cancer tissue at different anatomical locations and stages
Project Summary:Colorectal cancer (CRC) still remains the leading cause of cancer death worldwide. This study aimed to profile the metabolic differences of colorectal cancer tissues (CCT) at different stages and sites, as compared with their adjacent noncancerous tissues (ANT), to investigate the temporal and spatial heterogeneities of metabolic characterization. Our NMR-based metabolomics fingerprinting revealed that many of the metabolite levels were significantly altered in CCT compared to ANT and esophageal cancer tissues, indicating deregulations of glucose metabolism, one-carbon metabolism, glutamine metabolism, amino acid metabolism, fatty acid metabolism, TCA cycle, choline metabolism, etc. A total of five biomarker metabolites, including glucose, glutamate, alanine, valine and histidine, were identified to distinguish between early and advanced stages of CCT. Metabolites that distinguish the different anatomical sites of CCT include glucose, glycerol, glutamine, inositol, succinate, and citrate. Those significant metabolic differences in CRC tissues at different pathological stages and sites suggested temporal and spatial heterogeneities of metabolic characterization in CCT, providing a metabolic foundation for further study on biofluid metabolism in CRC early detection.
Institute:Radiology Department, Second Affiliated Hospital, Shantou University Medical College, Shantou
Last Name:Lin
First Name:Yan
Address:No. 69, Dongxia North Road, Shantou, Guangdong, China, Shantou, Guangdong, China, 515041, China
Email:994809889@qq.com
Phone:+86 18823992148

Subject:

Subject ID:SU003272
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Factor Factor
SA341098CRC-tissue-C0.49.fid#advanced CRC colon
SA341085CRC-tissue-C0.45.fid#advanced CRC CRC
SA341086CRC-tissue-C0.40.fid#advanced CRC CRC
SA341087CRC-tissue-C0.36.fid#advanced CRC LCC
SA341088CRC-tissue-C0.31.fid#advanced CRC LCC
SA341089CRC-tissue-C0.38.fid#advanced CRC LCC
SA341090CRC-tissue-C0.39.fid#advanced CRC LCC
SA341091CRC-tissue-C0.44.fid#advanced CRC LCC
SA341092CRC-tissue-C0.51.fid#advanced CRC RCC
SA341093CRC-tissue-C0.29.fid#advanced CRC RCC
SA341094CRC-tissue-C0.37.fid#advanced CRC RCC
SA341095CRC-tissue-C0.27.fid#advanced CRC RCC
SA341096CRC-tissue-C0.47.fid#advanced CRC RCC
SA341097CRC-tissue-C0.52.fid#advanced CRC RCC
SA341099CRC-tissue-C0.35.fid#advanced CRC rectum
SA341100CRC-tissue-C0.33.fid#advanced CRC rectum
SA341101CRC-tissue-C0.32.fid#advanced CRC rectum
SA341102CRC-tissue-C0.41.fid#advanced CRC rectum
SA341103CRC-tissue-C0.30.fid#advanced CRC rectum
SA341104CRC-tissue-C0.43.fid#advanced CRC rectum
SA341105CRC-tissue-C0.50.fid#advanced CRC rectum
SA341106CRC-tissue-C0.48.fid#advanced CRC rectum
SA341107CRC-tissue-C0.46.fid#advanced CRC rectum
SA341108CRC-tissue-C0.28.fid#advanced CRC rectum
SA341109CRC-tissue-C0.42.fid#advanced CRC rectum
SA341110CRC-tissue-C0.34.fid#advanced CRC rectum
SA341111CRC-tissue-C0.26.fid#advanced CRC rectum
SA341112CRC-tissue-P5.147.fid#advanced para-CRC CRC
SA341113CRC-tissue-P5.143.fid#advanced para-CRC CRC
SA341114CRC-tissue-P5.151.fid#advanced para-CRC LCC
SA341115CRC-tissue-P5.146.fid#advanced para-CRC LCC
SA341116CRC-tissue-P5.142.fid#advanced para-CRC LCC
SA341117CRC-tissue-P5.139.fid#advanced para-CRC LCC
SA341118CRC-tissue-P5.141.fid#advanced para-CRC LCC
SA341119CRC-tissue-P5.140.fid#advanced para-CRC RCC
SA341120CRC-tissue-P5.133.fid#advanced para-CRC RCC
SA341121CRC-tissue-P5.149.fid#advanced para-CRC RCC
SA341122CRC-tissue-P5.153.fid#advanced para-CRC RCC
SA341123CRC-tissue-P5.131.fid#advanced para-CRC RCC
SA341124CRC-tissue-P5.154.fid#advanced para-CRC RCC
SA341125CRC-tissue-P5.148.fid#advanced para-CRC rectum
SA341126CRC-tissue-P5.134.fid#advanced para-CRC rectum
SA341127CRC-tissue-P5.132.fid#advanced para-CRC rectum
SA341128CRC-tissue-P5.130.fid#advanced para-CRC rectum
SA341129CRC-tissue-P5.137.fid#advanced para-CRC rectum
SA341130CRC-tissue-P5.150.fid#advanced para-CRC rectum
SA341131CRC-tissue-P5.152.fid#advanced para-CRC rectum
SA341132CRC-tissue-P5.138.fid#advanced para-CRC rectum
SA341133CRC-tissue-P5.144.fid#advanced para-CRC rectum
SA341134CRC-tissue-P5.145.fid#advanced para-CRC rectum
SA341135CRC-tissue-P5.135.fid#advanced para-CRC rectum
SA341136CRC-tissue-P5.136.fid#advanced para-CRC rectum
SA341154CRC-tissue-C0.15.fid#early CRC colon
SA341155CRC-tissue-C0.14.fid#early CRC colon
SA341137CRC-tissue-C0.1.fid#early CRC CRC
SA341138CRC-tissue-C0.4.fid#early CRC LCC
SA341139CRC-tissue-C0.25.fid#early CRC LCC
SA341140CRC-tissue-C0.13.fid#early CRC LCC
SA341141CRC-tissue-C0.24.fid#early CRC LCC
SA341142CRC-tissue-C0.23.fid#early CRC LCC
SA341143CRC-tissue-C0.19.fid#early CRC LCC
SA341144CRC-tissue-C0.22.fid#early CRC LCC
SA341145CRC-tissue-C0.11.fid#early CRC LCC
SA341146CRC-tissue-C0.12.fid#early CRC LCC
SA341147CRC-tissue-C0.6.fid#early CRC LCC
SA341148CRC-tissue-C0.21.fid#early CRC RCC
SA341149CRC-tissue-C0.9.fid#early CRC RCC
SA341150CRC-tissue-C0.2.fid#early CRC RCC
SA341151CRC-tissue-C0.18.fid#early CRC RCC
SA341152CRC-tissue-C0.10.fid#early CRC RCC
SA341153CRC-tissue-C0.16.fid#early CRC RCC
SA341156CRC-tissue-C0.20.fid#early CRC rectum
SA341157CRC-tissue-C0.17.fid#early CRC rectum
SA341158CRC-tissue-C0.5.fid#early CRC rectum
SA341159CRC-tissue-C0.8.fid#early CRC rectum
SA341160CRC-tissue-C0.3.fid#early CRC rectum
SA341161CRC-tissue-C0.7.fid#early CRC rectum
SA341162CRC-tissue-P5.118.fid#early para-CRC CRC
SA341163CRC-tissue-P5.105.fid#early para-CRC CRC
SA341164CRC-tissue-P5.108.fid#early para-CRC LCC
SA341165CRC-tissue-P5.119.fid#early para-CRC LCC
SA341166CRC-tissue-P5.129.fid#early para-CRC LCC
SA341167CRC-tissue-P5.117.fid#early para-CRC LCC
SA341168CRC-tissue-P5.116.fid#early para-CRC LCC
SA341169CRC-tissue-P5.115.fid#early para-CRC LCC
SA341170CRC-tissue-P5.110.fid#early para-CRC LCC
SA341171CRC-tissue-P5.123.fid#early para-CRC LCC
SA341172CRC-tissue-P5.128.fid#early para-CRC LCC
SA341173CRC-tissue-P5.127.fid#early para-CRC LCC
SA341174CRC-tissue-P5.126.fid#early para-CRC LCC
SA341175CRC-tissue-P5.106.fid#early para-CRC RCC
SA341176CRC-tissue-P5.113.fid#early para-CRC RCC
SA341177CRC-tissue-P5.120.fid#early para-CRC RCC
SA341178CRC-tissue-P5.125.fid#early para-CRC RCC
SA341179CRC-tissue-P5.122.fid#early para-CRC RCC
SA341180CRC-tissue-P5.114.fid#early para-CRC RCC
SA341181CRC-tissue-P5.111.fid#early para-CRC rectum
SA341182CRC-tissue-P5.107.fid#early para-CRC rectum
SA341183CRC-tissue-P5.109.fid#early para-CRC rectum
SA341184CRC-tissue-P5.112.fid#early para-CRC rectum
Showing page 1 of 2     Results:    1  2  Next     Showing results 1 to 100 of 102

Collection:

Collection ID:CO003265
Collection Summary:Resected colorectal cancer tissues and their corresponding non-cancerous tissues were frozen in liquid nitrogen for 10s before being transferred to cryogenic vials and stored at −80°C until metabolite extraction.
Sample Type:colorectal tissue
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003281
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP003279
Sampleprep Summary:Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL) and distilled water (1.2 mL) were added and the sample was vortexed for 60 seconds. Finally, samples were incubated on ice for 15 mins, followed by centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with nitrogen and evaporated before incubating under vacuum for at least 18 hours. The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1 M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000 rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm size) for 1H NMR spectroscopy analysis.

Analysis:

Analysis ID:AN005176
Analysis Type:NMR
Results File:ST003155_AN005176_Results.txt
Units:peak area

NMR:

NMR ID:NM000279
Analysis ID:AN005176
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600 MHz
  logo