Summary of Study ST003156

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001962. The data can be accessed directly via it's Project DOI: 10.21228/M83X6D This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003156
Study TitleDiet modulates the protective effects of dimethyl fumarate mediated by the immunometabolic neutrophil receptor HCA2 - Metabolomics
Study TypeMetabolomics
Study SummaryMonomethyl fumarate (MMF) and its prodrug dimethyl fumarate (DMF) are currently the most widely used agents for the treatment of multiple sclerosis (MS). However, not all patients benefit from DMF. We hypothesized that the variable response of patients may be due to their diet. In support of this hypothesis, mice subjected to experimental autoimmune encephalomyelitis (EAE), a model of MS, did not benefit from DMF treatment when fed a lauric acid-rich (LA) diet, in contrast to normal chow (NC) or, more so, a high-fiber (HFb) diet. DMF lacked efficacy in the LA diet fed group despite similar resorption and preserved effects on plasma lipids.
Institute
University of Luebeck
DepartmentBioanalytic Core Facility
Last NameInderhees
First NameJulica
AddressRatzeburger Allee 160, 23562 Luebeck
Emailjulica.inderhees@uni-luebeck.de
Phone+4945131012805
Submit Date2024-04-04
Num Groups6
Total Subjects57
Num Females57
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Thermo)
Analysis Type DetailLC-MS
Release Date2025-03-31
Release Version1
Julica Inderhees Julica Inderhees
https://dx.doi.org/10.21228/M83X6D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001962
Project DOI:doi: 10.21228/M83X6D
Project Title:Diet modulates the protective effects of dimethyl fumarate mediated by the immunometabolic neutrophil receptor HCA2
Project Type:Metabolomics
Project Summary:Monomethyl fumarate (MMF) and its prodrug dimethyl fumarate (DMF) are currently the most widely used agents for the treatment of multiple sclerosis (MS). However, not all patients benefit from DMF. We hypothesized that the variable response of patients may be due to their diet. In support of this hypothesis, mice subjected to experimental autoimmune encephalomyelitis (EAE), a model of MS, did not benefit from DMF treatment when fed a lauric acid-rich (LA) diet, in contrast to normal chow (NC) or, more so, a high-fiber (HFb) diet. DMF lacked efficacy in the LA diet fed group despite similar resorption and preserved effects on plasma lipids.
Institute:University of Luebeck
Department:Bioanalytic Core Facility
Last Name:Inderhees
First Name:Julica
Address:Ratzburger Allee 160, Luebeck, Schleswig-Holstein, 23562, Germany
Email:julica.inderhees@uni-luebeck.de
Phone:+4945131012805
Contributors:Joanna Kosinska, Julian Assmann, Julica Inderhees

Subject:

Subject ID:SU003274
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:14-18 weeks
Weight Or Weight Range:18-23g
Gender:Female
Animal Animal Supplier:Charles River
Animal Housing:Standard conditions
Animal Light Cycle:12 hour light/dark cycle
Animal Feed:Mice were fed with HFb diet (35% fiber, Cat. C1000 modified, #100213, Altromin), LA diet (30% fat, Cat. C1000 modified, #100212, Altromin) or NC (14% Fat, 27% Protein, 59% Carbohydrates, Cat. #1314M, Altromin) ad libitum.
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet Treatment
SA341543Sample_16ctrl DMF
SA341544Sample_37ctrl DMF
SA341545Sample_15ctrl DMF
SA341546Sample_38ctrl DMF
SA341547Sample_14ctrl DMF
SA341548Sample_55ctrl DMF
SA341549Sample_13ctrl DMF
SA341550Sample_56ctrl DMF
SA341551Sample_40ctrl DMF
SA341552Sample_39ctrl DMF
SA341553Sample_01ctrl Veh
SA341554Sample_04ctrl Veh
SA341555Sample_03ctrl Veh
SA341556Sample_02ctrl Veh
SA341557Sample_25ctrl Veh
SA341558Sample_26ctrl Veh
SA341559Sample_49ctrl Veh
SA341560Sample_28ctrl Veh
SA341561Sample_27ctrl Veh
SA341562Sample_50ctrl Veh
SA341506Sample_41HFD DMF
SA341507Sample_20HFD DMF
SA341508Sample_19HFD DMF
SA341509Sample_42HFD DMF
SA341510Sample_44HFD DMF
SA341511Sample_58HFD DMF
SA341512Sample_57HFD DMF
SA341513Sample_17HFD DMF
SA341514Sample_43HFD DMF
SA341515Sample_18HFD DMF
SA341516Sample_32HFD Veh
SA341517Sample_51HFD Veh
SA341518Sample_05HFD Veh
SA341519Sample_52HFD Veh
SA341520Sample_30HFD Veh
SA341521Sample_31HFD Veh
SA341522Sample_06HFD Veh
SA341523Sample_08HFD Veh
SA341524Sample_07HFD Veh
SA341525Sample_29HFD Veh
SA341526Sample_48LA DMF
SA341527Sample_60LA DMF
SA341528Sample_47LA DMF
SA341529Sample_45LA DMF
SA341530Sample_46LA DMF
SA341531Sample_22LA DMF
SA341532Sample_24LA DMF
SA341533Sample_21LA DMF
SA341534Sample_12LA Veh
SA341535Sample_33LA Veh
SA341536Sample_10LA Veh
SA341537Sample_34LA Veh
SA341538Sample_11LA Veh
SA341539Sample_35LA Veh
SA341540Sample_54LA Veh
SA341541Sample_09LA Veh
SA341542Sample_36LA Veh
Showing results 1 to 57 of 57

Collection:

Collection ID:CO003267
Collection Summary:On the day of blood collection, 20 µl of fresh sodium floride (250 mg/ml, NaF) in water was added to each EDTA containing tube. After mice were deeply anaesthetized, blood was sampled from the right heart ventricle 20 min after DMG gavage and 500 µl were transferred to the prepared EDTA tube. After centrifugation (1,500 g) at 4 °C for 10 min, plasma was snap frozen in liquid nitrogen and stored at -80 °C until analysis.
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003283
Treatment Summary:Mice were fed with HFb diet (35% fiber, Cat. C1000 modified, #100213, Altromin), LA diet (30% fat, Cat. C1000 modified, #100212, Altromin) or NC (14% Fat, 27% Protein, 59% Carbohydrates, Cat. #1314M, Altromin) ad libitum. After 2 weeks EAE was induced by immunization with an emulsion of MOG35-55 with complete Freund’s adjuvant (CFA) (Hooke KitTM MOG35-55/CFA Emulsion PTX, EK-2110). The emulsion was injected subcutaneously at two sites in the upper and lower back (0.1 ml/site), followed by two intraperitoneal injections of pertussis toxin (PTX) diluted with ice-cold PBS (100 ng in 0.1 ml/dose). Mice were given DMF (50 mg/kg body weight) or vehicle orally by gavage every 12 hours. The treatment was provided until the end of the study (dpi 28).

Sample Preparation:

Sampleprep ID:SP003281
Sampleprep Summary:Thawed aliquots of plasma (50 µL) were subjected to solvent extraction once each with 200 µL of cold methanol/acetone/acetonitrile (1/1/1, v/v/v), containing 2.5 µM Metabolomics Amino Acid Mix Standard (Cambridge Isotope Laboratories, Andover, MA, United States) and mixed for 15 min at 4°C at 1000 rpm (Thermomixer Eppendorf), vortexed for 10 secs, and afterwards incubated for 2 h at 20°C. The mixture was then centrifuged for 10 min at 14,000 rpm at 4°C. The collected supernatants were evaporated to dryness in a vacuum concentrator (SpeedVac Concentrator, ThermoFisher). The dry extracts were reconstituted in 25 µL of methanol/acetonitrile (1:1) and vortexed for 15 sec followed by centrifugation at 14,000 rpm for 10 min at 4°C. The supernatants were transferred to LC-MS vials, and LC-HRMS analysis was performed.
Extract Storage:4℃

Chromatography:

Chromatography ID:CH003917
Chromatography Summary:Metabolites were separated on a SeQuant ZIC-HILIC column (150 × 2.1 mm, 5 μm; Merck) using water with 5 mM ammonium acetate as eluent A and acetonitrile/eluent A (95:5, v/v) as eluent B. The gradient elution was set as follows: isocratic step of 100% B for 3 min, 100% B to 60% B in 15 min, held for 5 min, returned to initial conditions in 5 min and held for 5 min. Flow rate was 0.5 ml/min.
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:SeQuant ZIC-HILIC (150 x 2.1mm,5um)
Column Temperature:35°C
Flow Gradient:isocratic step of 100% B for 3 min, 100% B to 60% B in 15 min, held for 5 min, returned to initial conditions in 5 min and held for 5 min.
Flow Rate:0.5ml/min
Solvent A:100% water; 5mM ammonium acetate
Solvent B:95% acetonitrile/5% (water; 5mM ammonium acetate)
Chromatography Type:HILIC

Analysis:

Analysis ID:AN005178
Laboratory Name:Bioanalytic Core Facility
Analysis Type:MS
Operator Name:Julica Inderhees
Chromatography ID:CH003917
Num Factors:6
Num Metabolites:91
Rt Units:Minutes
Units:relative area
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