Summary of Study ST003157

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001962. The data can be accessed directly via it's Project DOI: 10.21228/M83X6D This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003157
Study TitleDiet modulates the protective effects of dimethyl fumarate mediated by the immunometabolic neutrophil receptor HCA2 - Lipidomics
Study TypeLipidomics
Study SummaryMonomethyl fumarate (MMF) and its prodrug dimethyl fumarate (DMF) are currently the most widely used agents for the treatment of multiple sclerosis (MS). However, not all patients benefit from DMF. We hypothesized that the variable response of patients may be due to their diet. In support of this hypothesis, mice subjected to experimental autoimmune encephalomyelitis (EAE), a model of MS, did not benefit from DMF treatment when fed a lauric acid-rich (LA) diet, in contrast to normal chow (NC) or, more so, a high-fiber (HFb) diet. DMF lacked efficacy in the LA diet fed group despite similar resorption and preserved effects on plasma lipids.
Institute
University of Luebeck
DepartmentBioanalytic Core Facility
Last NameInderhees
First NameJulica
AddressRatzeburger Allee 160, 23562 Luebeck
Emailjulica.inderhees@uni-luebeck.de
Phone+4945131012805
Submit Date2024-04-04
Num Groups6
Total Subjects56
Num Females56
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Thermo)
Analysis Type DetailLC-MS
Release Date2025-03-31
Release Version1
Julica Inderhees Julica Inderhees
https://dx.doi.org/10.21228/M83X6D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001962
Project DOI:doi: 10.21228/M83X6D
Project Title:Diet modulates the protective effects of dimethyl fumarate mediated by the immunometabolic neutrophil receptor HCA2
Project Type:Metabolomics
Project Summary:Monomethyl fumarate (MMF) and its prodrug dimethyl fumarate (DMF) are currently the most widely used agents for the treatment of multiple sclerosis (MS). However, not all patients benefit from DMF. We hypothesized that the variable response of patients may be due to their diet. In support of this hypothesis, mice subjected to experimental autoimmune encephalomyelitis (EAE), a model of MS, did not benefit from DMF treatment when fed a lauric acid-rich (LA) diet, in contrast to normal chow (NC) or, more so, a high-fiber (HFb) diet. DMF lacked efficacy in the LA diet fed group despite similar resorption and preserved effects on plasma lipids.
Institute:University of Luebeck
Department:Bioanalytic Core Facility
Last Name:Inderhees
First Name:Julica
Address:Ratzburger Allee 160, Luebeck, Schleswig-Holstein, 23562, Germany
Email:julica.inderhees@uni-luebeck.de
Phone:+4945131012805
Contributors:Joanna Kosinska, Julian Assmann, Julica Inderhees

Subject:

Subject ID:SU003275
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:14-18 weeks
Weight Or Weight Range:18-23g
Gender:Female
Animal Animal Supplier:Charles River
Animal Housing:Standard conditions
Animal Light Cycle:12 hour light/dark cycle
Animal Feed:Mice were fed with HFb diet (35% fiber, Cat. C1000 modified, #100213, Altromin), LA diet (30% fat, Cat. C1000 modified, #100212, Altromin) or NC (14% Fat, 27% Protein, 59% Carbohydrates, Cat. #1314M, Altromin) ad libitum.
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet Treatment
SA341600Sample_16ctrl DMF
SA341601Sample_15ctrl DMF
SA341602Sample_14ctrl DMF
SA341603Sample_37ctrl DMF
SA341604Sample_40ctrl DMF
SA341605Sample_13ctrl DMF
SA341606Sample_56ctrl DMF
SA341607Sample_55ctrl DMF
SA341608Sample_39ctrl DMF
SA341609Sample_38ctrl DMF
SA341610Sample_01ctrl Veh
SA341611Sample_04ctrl Veh
SA341612Sample_03ctrl Veh
SA341613Sample_02ctrl Veh
SA341614Sample_25ctrl Veh
SA341615Sample_26ctrl Veh
SA341616Sample_28ctrl Veh
SA341617Sample_27ctrl Veh
SA341618Sample_49ctrl Veh
SA341563Sample_41HFD DMF
SA341564Sample_20HFD DMF
SA341565Sample_19HFD DMF
SA341566Sample_42HFD DMF
SA341567Sample_44HFD DMF
SA341568Sample_58HFD DMF
SA341569Sample_57HFD DMF
SA341570Sample_17HFD DMF
SA341571Sample_43HFD DMF
SA341572Sample_18HFD DMF
SA341573Sample_30HFD Veh
SA341574Sample_31HFD Veh
SA341575Sample_51HFD Veh
SA341576Sample_32HFD Veh
SA341577Sample_08HFD Veh
SA341578Sample_29HFD Veh
SA341579Sample_52HFD Veh
SA341580Sample_06HFD Veh
SA341581Sample_05HFD Veh
SA341582Sample_07HFD Veh
SA341583Sample_48LA DMF
SA341584Sample_60LA DMF
SA341585Sample_47LA DMF
SA341586Sample_45LA DMF
SA341587Sample_46LA DMF
SA341588Sample_22LA DMF
SA341589Sample_21LA DMF
SA341590Sample_24LA DMF
SA341591Sample_11LA Veh
SA341592Sample_10LA Veh
SA341593Sample_53LA Veh
SA341594Sample_33LA Veh
SA341595Sample_09LA Veh
SA341596Sample_34LA Veh
SA341597Sample_36LA Veh
SA341598Sample_54LA Veh
SA341599Sample_35LA Veh
Showing results 1 to 56 of 56

Collection:

Collection ID:CO003268
Collection Summary:On the day of blood collection, 20 µl of fresh sodium floride (250 mg/ml, NaF) in water was added to each EDTA containing tube. After mice were deeply anaesthetized, blood was sampled from the right heart ventricle 20 min after DMG gavage and 500 µl were transferred to the prepared EDTA tube. After centrifugation (1,500 g) at 4 °C for 10 min, plasma was snap frozen in liquid nitrogen and stored at -80 °C until analysis.
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003284
Treatment Summary:Mice were fed with HFb diet (35% fiber, Cat. C1000 modified, #100213, Altromin), LA diet (30% fat, Cat. C1000 modified, #100212, Altromin) or NC (14% Fat, 27% Protein, 59% Carbohydrates, Cat. #1314M, Altromin) ad libitum. After 2 weeks EAE was induced by immunization with an emulsion of MOG35-55 with complete Freund’s adjuvant (CFA) (Hooke KitTM MOG35-55/CFA Emulsion PTX, EK-2110). The emulsion was injected subcutaneously at two sites in the upper and lower back (0.1 ml/site), followed by two intraperitoneal injections of pertussis toxin (PTX) diluted with ice-cold PBS (100 ng in 0.1 ml/dose). Mice were given DMF (50 mg/kg body weight) or vehicle orally by gavage every 12 hours. The treatment was provided until the end of the study (dpi 28).

Sample Preparation:

Sampleprep ID:SP003282
Sampleprep Summary:Lipids were extracted by adding 1 ml of methanol/methyl tertiary-butyl ether/chloroform (1.33:1:1, v/v), containing butylated hydroxytoluene (100 mg/l, Sigma-Aldrich, Schnelldorf, Germany) and 2.5 ml/l of SPLASH internal standard mix (Avanti Polar Lipids, Alabaster, USA), to 50 µl of plasma. After incubation (30min/25°C/950rpm) and centrifugation (20°C/2,000g/10min), supernatants were dried under vacuum and resuspended in 50 µl of methanol/isopropyl alcohol (1:1, v/v) for LC-MS/MS analysis.
Extract Storage:4℃

Chromatography:

Chromatography ID:CH003918
Chromatography Summary:Extracted lipids were separated on an Accucore C30 RP column (150 × 2.1 mm, 2.6 µm; Thermo Fisher Scientific) using acetonitrile/water 6:4 (v/v) as eluent A and isopropyl alcohol/acetonitrile (9:1, v/v) as eluent B. Ammonium acetate and formic acid were added to both eluents.
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Thermo Acclaim C30 (150 x 2.1mm,3um)
Column Temperature:45°C
Flow Gradient:from 30 – 43 % B in 2.0 min, 43 – 55 % B in 0.1 min, to 65 % B in 9.9 min, to 85 % B in 6 min and to 100 % B in 2 min. An isocratic step of 100 % B was held for 15 min and returned to initial conditions in 0.1 min and held for 2.9 min
Flow Rate:0.26ml/min
Solvent A:acetonitrile/water 6:4; 10mM ammonium acetate; 0.1% formic acid
Solvent B:Isopropyl alcohol/acetonitrile 9:1; 10mM ammonium acetate; 0.1% formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN005179
Laboratory Name:Bioanalytic Core Facility
Analysis Type:MS
Operator Name:Julica Inderhees
Chromatography ID:CH003918
Num Factors:6
Num Metabolites:656
Rt Units:Minutes
Units:relative area
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