Summary of Study ST003212
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002003. The data can be accessed directly via it's Project DOI: 10.21228/M8Q237 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003212 |
| Study Title | Using Untargeted Metabolomics as a New Approach for Understanding Honeybee Toxicity |
| Study Summary | The impact of crop protection products on pollinating insects, particularly honeybees, is a critical consideration for ecological safety. Beyond acute toxicity, sublethal effects can potentially disrupt honeybee behavior, compromising pollination efficiency. Traditional assessment methods, reliant on visual observations, may not be optimal for capturing sublethal effects. In this study, we delved into molecular mechanisms underlying sublethal effects, employing three distinct platforms: whole-tissue transcriptomics, spatial transcriptomics, and metabolomics. Our findings reveal valuable insights into honeybee toxicity, with certain approaches demonstrating superiority in elucidating tissue-specific and treatment-specific responses, especially untargeted metabolomics tend to be a valuable tool for new bee-friendly agrochemical development. This research will aid in screening new molecules for pollinator safety and contribute to developing strategies that enhance crop yield while maintaining ecological sustainability. |
| Institute | Corteva Agriscience |
| Last Name | Yang |
| First Name | Kundi |
| Address | 9330 Zionsville Rd, Indianapolis, IN 46268, Indianapolis, Indiana, 46077, USA |
| kundi.yang@corteva.com | |
| Phone | 5133063039 |
| Submit Date | 2024-05-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-12-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002003 |
| Project DOI: | doi: 10.21228/M8Q237 |
| Project Title: | Honeybee metabolomics 2022 for Understanding Honeybee Toxicity |
| Project Type: | Untargeted metabolomics |
| Project Summary: | The impact of crop protection products on pollinating insects, particularly honeybees, is a critical consideration for ecological safety. Beyond acute toxicity, sublethal effects can potentially disrupt honeybee behavior, compromising pollination efficiency. Traditional assessment methods, reliant on visual observations, may not be optimal for capturing sublethal effects. In this study, we delved into molecular mechanisms underlying sublethal effects, employing three distinct platforms: whole-tissue transcriptomics, spatial transcriptomics, and metabolomics. Our findings reveal valuable insights into honeybee toxicity, with certain approaches demonstrating superiority in elucidating tissue-specific and treatment-specific responses, especially untargeted metabolomics tend to be a valuable tool for new bee-friendly agrochemical development. This research will aid in screening new molecules for pollinator safety and contribute to developing strategies that enhance crop yield while maintaining ecological sustainability. |
| Institute: | Corteva Agriscience |
| Last Name: | Yang |
| First Name: | Kundi |
| Address: | 9330 Zionsville Rd, Indianapolis, IN 46268, Indianapolis, Indiana, 46077, USA |
| Email: | kundi.yang@corteva.com |
| Phone: | 5133063039 |
Subject:
| Subject ID: | SU003331 |
| Subject Type: | Insect |
| Subject Species: | Apis mellifera |
| Taxonomy ID: | 7460 |
| Species Group: | Insects |
Factors:
Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Sample section |
|---|---|---|---|
| SA351691 | A-2 | Abdomen | Wild Type_Abdomen |
| SA351692 | A-1 | Abdomen | Wild Type_Abdomen |
| SA351693 | A-3 | Abdomen | Wild Type_Abdomen |
| SA351694 | A-4 | Abdomen | Wild Type_Abdomen |
| SA351695 | A-5 | Abdomen | Wild Type_Abdomen |
| SA351696 | H-4 | Head | Wild Type_Head |
| SA351697 | H-3 | Head | Wild Type_Head |
| SA351698 | H-5 | Head | Wild Type_Head |
| SA351699 | H-1 | Head | Wild Type_Head |
| SA351700 | H-2 | Head | Wild Type_Head |
| SA351701 | QC2 | Pooled Wild Type | Pooled Wild Type |
| SA351702 | QC1 | Pooled Wild Type | Pooled Wild Type |
| SA351703 | QC3 | Pooled Wild Type | Pooled Wild Type |
| SA351704 | Blank2 | SolventBlank | Blank |
| SA351705 | Blank4 | SolventBlank | Blank |
| SA351706 | Blank1 | SolventBlank | Blank |
| SA351707 | T-5 | Thorax | Wild Type_Thorax |
| SA351708 | T-1 | Thorax | Wild Type_Thorax |
| SA351709 | T-2 | Thorax | Wild Type_Thorax |
| SA351710 | T-3 | Thorax | Wild Type_Thorax |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO003324 |
| Collection Summary: | Honeybee were weighted and sectioned. |
| Sample Type: | Tissues |
Treatment:
| Treatment ID: | TR003340 |
| Treatment Summary: | Honeybee were treated with normal diet. |
Sample Preparation:
| Sampleprep ID: | SP003338 |
| Sampleprep Summary: | Honeybee sections were collected separately and then homogenised with stainless steel balls. Organic/Water extraction were used for metabolite extraction. |
Chromatography:
| Chromatography ID: | CH003986 |
| Chromatography Summary: | Solvent A: Acetonitrile/Water 90% / 10% (v/v) + 0.2% Acetic acid (v) + 5 mM Ammonium acetate; Solvent B: Acetonitrile/Water 10% / 90% (v/v) + 0.2% Acetic acid (v) + 5 mM Ammonium acetate; Flow Gradient: 0 - 1 min, 2% B, ramping up to 75% B from 1 min to 6 min, then immediately a steeper gradient was applied to ramp the %B to 98% at 12 min, 12 – 15 min, 98% B |
| Chromatography Comments: | Atlantis Premier BEH Z-HILIC Cloumn, 1.7 um, 2.1 mm x 100 mm |
| Instrument Name: | Thermo Scientific Dionex Ultimate 3000 |
| Column Name: | Waters Atlantis Premier BEH Z-HILIC (100 x 2.1 mm, 1.7 um) |
| Column Temperature: | 25 |
| Flow Gradient: | 0 - 1 min, 2% B; 6 min, 75% B; 12 min, 98% B; 12 – 15 min, 98% B |
| Flow Rate: | 0.25mL/min |
| Solvent A: | 90% acetonitrile/10% water; 0.2% acetic acid; 5 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/90% water; 0.2% acetic acid; 5 mM ammonium acetate |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN005267 |
| Analysis Type: | MS |
| Chromatography ID: | CH003986 |
| Has Mz: | 1 |
| Rt Units: | No RT data |
| Results File: | ST003212_AN005267_Results.txt |
| Units: | counts |
| Analysis ID: | AN005268 |
| Analysis Type: | MS |
| Chromatography ID: | CH003986 |
| Has Mz: | 1 |
| Rt Units: | No RT data |
| Results File: | ST003212_AN005268_Results.txt |
| Units: | counts |
