Summary of Study ST003299
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003299 |
| Study Title | Lactiplantibacillus plantarum intervention on fecal tryptophans of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of fecal tryptophans of germ-free mice. L. plantarum intervention during gestation of humanized GF mice showed that serotonin was significantly increased, other tryptophans no significant difference. |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai district |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-12-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001923 |
| Project DOI: | doi: 10.21228/M85149 |
| Project Title: | Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice |
| Project Summary: | The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism. |
| Institute: | Beijing Sanyuan Foods Co. Ltd |
| Last Name: | Lijun |
| First Name: | Chen |
| Address: | 8th yingchang street, yinghai distric |
| Email: | chenlijun@sanyuan.com.cn |
| Phone: | 01056306594 |
Subject:
| Subject ID: | SU003420 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA358070 | 908S0 | control |
| SA358071 | 185S18 | control |
| SA358072 | 185S7 | control |
| SA358073 | 188S0 | control |
| SA358074 | 188S1 | control |
| SA358075 | 188S14 | control |
| SA358076 | 188S18 | control |
| SA358077 | 188S7 | control |
| SA358078 | 908S1 | control |
| SA358079 | 185S1 | control |
| SA358080 | 908S14 | control |
| SA358081 | 908S18 | control |
| SA358082 | 908S7 | control |
| SA358083 | 909S0 | control |
| SA358084 | 909S1 | control |
| SA358085 | 909S14 | control |
| SA358086 | 909S18 | control |
| SA358087 | 909S7 | control |
| SA358088 | 185S14 | control |
| SA358089 | 185S0 | control |
| SA358090 | 180S1 | intervention |
| SA358091 | 907S14 | intervention |
| SA358092 | 081S14 | intervention |
| SA358093 | 081S18 | intervention |
| SA358094 | 081S7 | intervention |
| SA358095 | 107S0 | intervention |
| SA358096 | 107S1 | intervention |
| SA358097 | 107S14 | intervention |
| SA358098 | 107S18 | intervention |
| SA358099 | 107S7 | intervention |
| SA358100 | 120S0 | intervention |
| SA358101 | 120S1 | intervention |
| SA358102 | 907S7 | intervention |
| SA358103 | 907S18 | intervention |
| SA358104 | 907S1 | intervention |
| SA358105 | 180S14 | intervention |
| SA358106 | 907S0 | intervention |
| SA358107 | 120S14 | intervention |
| SA358108 | 120S18 | intervention |
| SA358109 | 120S7 | intervention |
| SA358110 | 122S0 | intervention |
| SA358111 | 122S1 | intervention |
| SA358112 | 122S14 | intervention |
| SA358113 | 122S18 | intervention |
| SA358114 | 122S7 | intervention |
| SA358115 | 180S0 | intervention |
| SA358116 | 081S1 | intervention |
| SA358117 | 180S7 | intervention |
| SA358118 | 180S18 | intervention |
| SA358119 | 081S0 | intervention |
| Showing results 1 to 50 of 50 |
Collection:
| Collection ID: | CO003413 |
| Collection Summary: | Feces were collected at and stored at -80°C for tryptophan analysis. |
| Sample Type: | Feces |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003429 |
| Treatment Summary: | Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003427 |
| Sampleprep Summary: | Sample was extracted in 100 μL of 80% methanol aqueous solution, high throughput tissue grinder grinding for 60 s, then add 900 μL of 10% methanol aqueous solution, high throughput tissue grinder grinding for 120 s. Centrifugation was carried out at 12,000 rpm at 4℃ for 10 min, and the supernatant was filtered through the membrane to add an equal amount of the internal standard solution, and then transferred into the vial |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Chromatography:
| Chromatography ID: | CH004099 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0-2 min, 1% B; 2-3 min, 1-30% B; 3-3.5 min, 30% B; 4.5-8 min, 30-50% B; 8-10 min, 50-95% B; 10-11 min, 95% B; 11-17 min, 95-1% B. |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% Water; 0.1% Formic acid |
| Solvent B: | 100% Methanol; 0.1% Formic acid |
| Chromatography Type: | LC |
Analysis:
| Analysis ID: | AN005406 |
| Analysis Type: | MS |
| Chromatography ID: | CH004099 |
| Num Factors: | 2 |
| Num Metabolites: | 21 |
| Units: | μg/g |
