Summary of Study ST003512

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002157. The data can be accessed directly via it's Project DOI: 10.21228/M8T24J This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003512
Study TitleExogenous L-serine supply protects against retinopathy of prematurity in a murine model
Study SummaryPathological retinal angiogenesis with irregular and fragile vessels (also termed as neovascularization, a response to hypoxia and dysmetabolism) is a leading cause of vision loss in all age groups driven in part by unmet metabolic demand from retinal neurons. Sustaining neural retinal metabolism with an adequate nutrient supply may prevent vision-threatening neovascularization. Low circulating serine levels are associated with neovascularization in macular telangiectasia and altered serine/glycine metabolism is suggested in retinopathy of prematurity. Here we assessed the role of serine metabolism in suppressing hypoxia-driven retinal neovascularization in mice. Serine or vehicle control was supplemented systemically via intraperitoneal injection. Systemic serine supplementation decreased retinal neovascularization. Metabolomic analysis showed serine treatment increased metabolites involved in fatty acid oxidation and phospholipid synthesis.
Institute
Boston Children's Hospital
Last NameFu
First NameZhongjie
Address1 Blackfan Circle, Boston, MA 02114
EmailZhongjie.Fu@childrens.harvard.edu
Phone6179192534
Submit Date2024-09-11
Num Groups2
Total Subjects6
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2025-05-01
Release Version1
Zhongjie Fu Zhongjie Fu
https://dx.doi.org/10.21228/M8T24J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002157
Project DOI:doi: 10.21228/M8T24J
Project Title:Exogenous L-serine supply protects against retinopathy of prematurity in a murine model
Project Summary:Pathological retinal angiogenesis with irregular and fragile vessels (also termed as neovascularization, a response to hypoxia and dysmetabolism) is a leading cause of vision loss in all age groups driven in part by unmet metabolic demand from retinal neurons. Sustaining neural retinal metabolism with an adequate nutrient supply may prevent vision-threatening neovascularization. Low circulating serine levels are associated with neovascularization in macular telangiectasia and altered serine/glycine metabolism is suggested in retinopathy of prematurity. Here we assessed the role of serine metabolism in suppressing hypoxia-driven retinal neovascularization in mice. Serine or vehicle control was supplemented systemically via intraperitoneal injection. Systemic serine supplementation decreased retinal neovascularization. Metabolomic analysis showed serine treatment increased metabolites involved in fatty acid oxidation and phospholipid synthesis.
Institute:Boston Children's Hospital
Last Name:Fu
First Name:Zhongjie
Address:1 Blackfan Circle, Boston, MA 02114
Email:Zhongjie.Fu@childrens.harvard.edu
Phone:617-919-2534

Subject:

Subject ID:SU003641
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Treatment
SA386092Blank_0Blank -
SA386093Blank_1Blank -
SA386094Blank_2Blank -
SA386095Blank_3Blank -
SA386096Blank_4Blank -
SA386097Blank_5Blank -
SA386098ISTD_0Internal Std -
SA386099ISTD_1Internal Std -
SA386100ISTD_2Internal Std -
SA386101PBS-1Retina PBS
SA386102PBS-2Retina PBS
SA386103PBS-3Retina PBS
SA386104Serine-1Retina Serine
SA386105Serine-2Retina Serine
SA386106Serine-3Retina Serine
Showing results 1 to 15 of 15

Collection:

Collection ID:CO003634
Collection Summary:Mouse retinas were collected at postnatal day 17 (P17) in the mouse model of oxygen-induced retinopathy (OIR). The study was conducted so that there would be three replicates in both the experimental and control groups.
Sample Type:Retina

Treatment:

Treatment ID:TR003650
Treatment Summary:OIR mouse pups were treated with serine (0.6 ug/g) or vehicle control intraperitoneally from postnatal day 12 (P12) to 16 (P16). At Postnatal day 17 (P17), retinas were collected for metabolomics.

Sample Preparation:

Sampleprep ID:SP003648
Sampleprep Summary:Both retinas from each mouse were collected, pooled, and prepared for targeted MS-based metabolomics by flash-freezing harvested samples and storing at -80*C until processed by metabolomics core.
Sampleprep Protocol ID:SP002600

Chromatography:

Chromatography ID:CH004375
Chromatography Summary:Solvent A was at pH 9.0; 2uL of sample were injected onto pHILIC column with a gradient of 80% ACN dropping to 20% acetonitrile over a 30 minute period, followed by a return and hold to 80% acetonitrile.
Instrument Name:Thermo Ultimate 3000
Column Name:SeQuant ZIC-pHILIC (150 x 2.1mm, 5um)
Column Temperature:25
Flow Gradient:80-20%B (0-30 min), 20-80%B (30-31 min), 80-80%B (31-42 min)
Flow Rate:0.1mL/min
Solvent A:100% water; 10 mM ammonium carbonate
Solvent B:100% acetonitrile
Chromatography Type:HILIC

Analysis:

Analysis ID:AN005766
Laboratory Name:Metabolomics Core Resource Laboratory
Analysis Type:MS
Software Version:4.1
Chromatography ID:CH004375
Num Factors:4
Num Metabolites:483
Rt Units:Minutes
Units:intensity
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