Summary of Study ST003534
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002168. The data can be accessed directly via it's Project DOI: 10.21228/M8CR8X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003534 |
| Study Title | Metabolomic analysis of altered arachidonic acid metabolism in mouse heart tissue after myocardial infarction |
| Study Summary | We performed myocardial infarction surgery on wild-type (WT) mice and collected cardiac tissues from the sham group and 1, 7, 14 and 28 days after surgery for LC-MS analysis. The results showed that AA-derived LTB4 was produced in large quantities 2 weeks after myocardial infarction and remained at a high level for a long time. |
| Institute | Tianjin Medical University |
| Last Name | Song |
| First Name | Jiawei |
| Address | Tianjin Medical University, Tianjin, China., Tianjin, Tianjin, 300070, China |
| sjw808@tmu.edu.cn | |
| Phone | 15309216256 |
| Submit Date | 2024-10-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-11-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002168 |
| Project DOI: | doi: 10.21228/M8CR8X |
| Project Title: | Arachidonic acid fuels inflammation by unlocking 5-LOX in macrophages after myocardial infarction to promote cardiac dysfunction |
| Project Summary: | An overactive inflammatory response and immune cell infiltration in myocardial infarction (MI) impairs tissue repair. Arachidonic acid (AA) metabolites act as important sources of inflammatory mediators in cardiac tissue. We investigated the mechanisms underlying the AA cascade-mediated inflammatory response after MI. Metabolomics analyses revealed that the 5-lipoxygenase (5-LOX)-dependent AA metabolic pathway derivative leukotriene B4 (LTB4) accumulated in large quantities after MI. Elevated levels of AA in vivo after MI facilitated the recruitment of protein phosphatase 5 (PP5) into the nucleus by binding PP5 to its nuclear transporter receptor karyopherin subunit alpha 1. On entering the nucleus, PP5 directly dephosphorylated 5-LOX at Thr218, promoting the production of the proinflammatory mediator LTB4 by macrophages. Transgenic mice were created with a population of cardiac resident macrophages with a mutation at 5-LOX Thr218. Single-cell transcriptomics showed that these cells overexpressed CXC-chemokine ligand 13 and actively recruited B cells to the heart, exacerbating tissue inflammation. In a UK Biobank cohort, the PP5 E76D mutation was found to be associated with complications after acute MI. Overall, our results have elucidated a conserved role for 5-LOX-phosphorylation-regulated LTB4 levels in MI and identified PP5 as a potential therapeutic target for the treatment of MI. |
| Institute: | Tianjin Medical University |
| Last Name: | Song |
| First Name: | Jiawei |
| Address: | Tianjin Medical University, Tianjin, China., Tianjin, Tianjin, 300070, China |
| Email: | sjw808@tmu.edu.cn |
| Phone: | 15309216256 |
Subject:
| Subject ID: | SU003663 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | treatment |
|---|---|---|
| SA387390 | MI0d_02 | post-MI day0 |
| SA387391 | MI0d_07_arachidonic acid | post-MI day0 |
| SA387392 | MI0d_06_arachidonic acid | post-MI day0 |
| SA387393 | MI0d_05_arachidonic acid | post-MI day0 |
| SA387394 | MI0d_04_arachidonic acid | post-MI day0 |
| SA387395 | MI0d_03_arachidonic acid | post-MI day0 |
| SA387396 | MI0d_02_arachidonic acid | post-MI day0 |
| SA387397 | MI0d_01 | post-MI day0 |
| SA387398 | MI0d_01_arachidonic acid | post-MI day0 |
| SA387399 | MI0d_07 | post-MI day0 |
| SA387400 | MI0d_03 | post-MI day0 |
| SA387401 | MI0d_05 | post-MI day0 |
| SA387402 | MI0d_06 | post-MI day0 |
| SA387403 | MI0d_04 | post-MI day0 |
| SA387418 | MI1d_04_arachidonic acid | post-MI day1 |
| SA387419 | MI1d_01_arachidonic acid | post-MI day1 |
| SA387420 | MI1d_03_arachidonic acid | post-MI day1 |
| SA387421 | MI1d_03 | post-MI day1 |
| SA387422 | MI1d_05_arachidonic acid | post-MI day1 |
| SA387423 | MI1d_06_arachidonic acid | post-MI day1 |
| SA387424 | MI1d_07_arachidonic acid | post-MI day1 |
| SA387425 | MI1d_04 | post-MI day1 |
| SA387426 | MI1d_05 | post-MI day1 |
| SA387427 | MI1d_02_arachidonic acid | post-MI day1 |
| SA387428 | MI1d_06 | post-MI day1 |
| SA387429 | MI1d_01 | post-MI day1 |
| SA387430 | MI1d_02 | post-MI day1 |
| SA387431 | MI1d_07 | post-MI day1 |
| SA387404 | MI14d_06 | post-MI day14 |
| SA387405 | MI14d_04 | post-MI day14 |
| SA387406 | MI14d_01_arachidonic acid | post-MI day14 |
| SA387407 | MI14d_02_arachidonic acid | post-MI day14 |
| SA387408 | MI14d_03_arachidonic acid | post-MI day14 |
| SA387409 | MI14d_07 | post-MI day14 |
| SA387410 | MI14d_05 | post-MI day14 |
| SA387411 | MI14d_03 | post-MI day14 |
| SA387412 | MI14d_02 | post-MI day14 |
| SA387413 | MI14d_01 | post-MI day14 |
| SA387414 | MI14d_04_arachidonic acid | post-MI day14 |
| SA387415 | MI14d_05_arachidonic acid | post-MI day14 |
| SA387416 | MI14d_06_arachidonic acid | post-MI day14 |
| SA387417 | MI14d_07_arachidonic acid | post-MI day14 |
| SA387432 | MI28d_04_arachidonic acid | post-MI day28 |
| SA387433 | MI28d_02 | post-MI day28 |
| SA387434 | MI28d_03 | post-MI day28 |
| SA387435 | MI28d_01_arachidonic acid | post-MI day28 |
| SA387436 | MI28d_02_arachidonic acid | post-MI day28 |
| SA387437 | MI28d_03_arachidonic acid | post-MI day28 |
| SA387438 | MI28d_05_arachidonic acid | post-MI day28 |
| SA387439 | MI28d_04 | post-MI day28 |
| SA387440 | MI28d_05 | post-MI day28 |
| SA387441 | MI28d_06 | post-MI day28 |
| SA387442 | MI28d_07 | post-MI day28 |
| SA387443 | MI28d_06_arachidonic acid | post-MI day28 |
| SA387444 | MI28d_07_arachidonic acid | post-MI day28 |
| SA387445 | MI28d_01 | post-MI day28 |
| SA387446 | MI7d_05 | post-MI day7 |
| SA387447 | MI7d_04 | post-MI day7 |
| SA387448 | MI7d_07_arachidonic acid | post-MI day7 |
| SA387449 | MI7d_06 | post-MI day7 |
| SA387450 | MI7d_07 | post-MI day7 |
| SA387451 | MI7d_06_arachidonic acid | post-MI day7 |
| SA387452 | MI7d_05_arachidonic acid | post-MI day7 |
| SA387453 | MI7d_04_arachidonic acid | post-MI day7 |
| SA387454 | MI7d_03_arachidonic acid | post-MI day7 |
| SA387455 | MI7d_02_arachidonic acid | post-MI day7 |
| SA387456 | MI7d_01_arachidonic acid | post-MI day7 |
| SA387457 | MI7d_03 | post-MI day7 |
| SA387458 | MI7d_01 | post-MI day7 |
| SA387459 | MI7d_02 | post-MI day7 |
| Showing results 1 to 70 of 70 |
Collection:
| Collection ID: | CO003656 |
| Collection Summary: | Myocardial infarction (MI) was induced in 8-week-old male or female C57BL/6J mice by permanent left anterior descending coronary artery ligation. Mice were then killed by intraperitoneal injection of avertin (30 μl g-1), and cardiac tissue was isolated by saline perfusion through the left ventricle after removal of the right auricle. |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR003672 |
| Treatment Summary: | Myocardial infarction (MI) was induced in 8-week-old male or female C57BL/6J mice by permanent left anterior descending coronary artery ligation. Mice were then killed by intraperitoneal injection of avertin (30 μl g-1) at 1, 7, 14, and 28 days after infarction, respectively. Cardiac tissue was isolated by saline perfusion of the left ventricle after excision of the right auricle. |
Sample Preparation:
| Sampleprep ID: | SP003670 |
| Sampleprep Summary: | A 50-mg sample of mouse heart tissue was extracted using a centrifuge tube with low adsorption properties after adding 1 µl of internal standard mixture. Arachidonic acid (AA) metabolites were extracted with methanol and ethyl acetate under low light conditions. The extracted samples were evaporated to dryness in a gentle stream of nitrogen, after which the samples were dissolved in 30% acetonitrile. |
Chromatography:
| Chromatography ID: | CH004408 |
| Chromatography Summary: | Chromatographic separation was performed on an UPLC BEH C18 column (1.7 µm, 50 × 2.1 mm i.d). |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-1min, 25% B; 1-8min, 25% B-95% B; 8-8.5min, 95% B; 8.5-8.51min, 95% B-25% B; 8.51-9min, 25%B |
| Flow Rate: | 0.6mL/min |
| Solvent A: | 100% water |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN005805 |
| Analysis Type: | MS |
| Chromatography ID: | CH004408 |
| Num Factors: | 5 |
| Num Metabolites: | 72 |
| Units: | ng/mL |
