Summary of Study ST003552

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002188. The data can be accessed directly via it's Project DOI: 10.21228/M8SV5J This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003552
Study TitleInvestigation of age-dependent changes in the gut of honeybee workers using targeted metabolomics for SCFA determination
Study SummaryTo determine changes in the metabolic activity of the gut microbiota, we measured the concentrations of seven short-chain fatty acids (SCFA) and lactate. All SCFA showed low concentrations in newly emerged workers. We found that acetate, propionate and butyrate are the main SCFA in the honeybee gut and peak in 5-day old and in-hive workers. Lactate showed an inverse trend with highest concentrations in 1-day old workers and a significant decrease on all subsequent days (Kruskal Wallis test, p <0.05).
Institute
Helmholtz Centre for Environmental Research
DepartmentMolecular Systems Biology
Last NameEngelmann
First NameBeatrice
AddressPermoserstraße 15, Leipzipg, Saxony, 03418, Germany
Emailbeatrice.engelmann@ufz.de
Phone004934160251099
Submit Date2024-10-21
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-11-27
Release Version1
Beatrice Engelmann Beatrice Engelmann
https://dx.doi.org/10.21228/M8SV5J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002188
Project DOI:doi: 10.21228/M8SV5J
Project Title:Comprehension of the age-dependent gut and brain interaction of honeybee workers by integration of multi omics approaches
Project Summary:In honeybees, division of labour is a key feature, with age-related behavioural transitions being closely associated with molecular changes in the brain, gut, and microbiota. In this study, to investigate these molecular changes and thus better understand their contribution to behavioural responses and modulation, we analysed the global metabolomic shifts in honeybee workers and their microbiota throughout their lives. Overall, our findings provide new insights toward developing potential biomarkers for evaluation of different functional changes related to various environmental stressors.
Institute:Helmholtz Centre for Environmental Research
Department:Molecular Systems Biology
Last Name:Engelmann
First Name:Beatrice
Address:Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
Email:beatrice.engelmann@ufz.de
Phone:004934160251099

Subject:

Subject ID:SU003681
Subject Type:Insect
Subject Species:Apis mellifera
Taxonomy ID:7460
Species Group:Insects

Factors:

Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)

mb_sample_id local_sample_id life stage age of workers in days
SA387919H2_D1_4_dilutedestablishment 1
SA387920H2_D1_4establishment 1
SA387921H2_D1_5establishment 1
SA387922H3_D1_1establishment 1
SA387923H3_D1_2establishment 1
SA387924H3_D1_3establishment 1
SA387925H3_D1_4establishment 1
SA387926H3_D1_5establishment 1
SA387927H2_D1_3_dilutedestablishment 1
SA387928H2_D1_1establishment 1
SA387929H2_D1_2_dilutedestablishment 1
SA387930H2_D1_1_dilutedestablishment 1
SA387931H1_D1_5_dilutedestablishment 1
SA387932H1_D1_4_dilutedestablishment 1
SA387933H1_D1_3_dilutedestablishment 1
SA387934H1_D1_2_dilutedestablishment 1
SA387935H1_D1_1_dilutedestablishment 1
SA387936H2_D1_2establishment 1
SA387937H2_D1_3establishment 1
SA387938H1_D1_5establishment 1
SA387939H3_D1_1_dilutedestablishment 1
SA387940H1_D1_4establishment 1
SA387941H3_D1_5_dilutedestablishment 1
SA387942H3_D1_4_dilutedestablishment 1
SA387943H3_D1_3_dilutedestablishment 1
SA387944H3_D1_2_dilutedestablishment 1
SA387945H2_D1_5_dilutedestablishment 1
SA387946H1_D1_1establishment 1
SA387947H1_D1_2establishment 1
SA387948H1_D1_3establishment 1
SA387949H2_D3_2_dilutedestablishment 3
SA387950H3_D3_4_dilutedestablishment 3
SA387951H3_D3_3_dilutedestablishment 3
SA387952H3_D3_2_dilutedestablishment 3
SA387953H3_D3_1_dilutedestablishment 3
SA387954H2_D3_5_dilutedestablishment 3
SA387955H1_D3_1_dilutedestablishment 3
SA387956H2_D3_1_dilutedestablishment 3
SA387957H1_D3_5_dilutedestablishment 3
SA387958H1_D3_4_dilutedestablishment 3
SA387959H1_D3_3_dilutedestablishment 3
SA387960H1_D3_2_dilutedestablishment 3
SA387961H2_D3_4_dilutedestablishment 3
SA387962H3_D3_5_dilutedestablishment 3
SA387963H2_D3_3_dilutedestablishment 3
SA387964H3_D3_2establishment 3
SA387965H2_D3_5establishment 3
SA387966H1_D3_1establishment 3
SA387967H1_D3_2establishment 3
SA387968H1_D3_3establishment 3
SA387969H1_D3_4establishment 3
SA387970H1_D3_5establishment 3
SA387971H2_D3_1establishment 3
SA387972H2_D3_2establishment 3
SA387973H3_D3_5establishment 3
SA387974H3_D3_4establishment 3
SA387975H3_D3_3establishment 3
SA387976H2_D3_3establishment 3
SA387977H2_D3_4establishment 3
SA387978H3_D3_1establishment 3
SA387979H1_D5_2establishment 5
SA387980H3_D5_1_dilutedestablishment 5
SA387981H1_D5_3establishment 5
SA387982H1_D5_1_dilutedestablishment 5
SA387983H1_D5_2_dilutedestablishment 5
SA387984H1_D5_3_dilutedestablishment 5
SA387985H2_D5_4_dilutedestablishment 5
SA387986H2_D5_3_dilutedestablishment 5
SA387987H2_D5_2_dilutedestablishment 5
SA387988H2_D5_1_dilutedestablishment 5
SA387989H1_D5_4_dilutedestablishment 5
SA387990H2_D5_5_dilutedestablishment 5
SA387991H3_D5_2_dilutedestablishment 5
SA387992H3_D5_5establishment 5
SA387993H1_D5_4establishment 5
SA387994H1_D5_5establishment 5
SA387995H2_D5_1establishment 5
SA387996H2_D5_2establishment 5
SA387997H2_D5_3establishment 5
SA387998H2_D5_4establishment 5
SA387999H2_D5_5establishment 5
SA388000H3_D5_1establishment 5
SA388001H3_D5_2establishment 5
SA388002H3_D5_3establishment 5
SA388003H3_D5_4establishment 5
SA388004H1_D5_5_dilutedestablishment 5
SA388005H3_D5_3_dilutedestablishment 5
SA388006H1_D5_1establishment 5
SA388007H3_D5_5_dilutedestablishment 5
SA388008H3_D5_4_dilutedestablishment 5
SA388009H3_EF_5_dilutedexperienced_forager >12
SA388010H3_EF_3_dilutedexperienced_forager >12
SA388011H3_EF_2_dilutedexperienced_forager >12
SA388012H3_EF_1_dilutedexperienced_forager >12
SA388013H2_EF_5_dilutedexperienced_forager >12
SA388014H2_EF_4_dilutedexperienced_forager >12
SA388015H2_EF_3_dilutedexperienced_forager >12
SA388016H1_EF_1_dilutedexperienced_forager >12
SA388017H2_EF_1_dilutedexperienced_forager >12
SA388018H2_EF_2_dilutedexperienced_forager >12
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Collection:

Collection ID:CO003674
Collection Summary:Workers were pooled to obtain a final sample number of n = 5. The brain(s) and gut(s) from the same workers were assigned the same sample names. In total, we had 15 samples from each age group, with the exception of the foraging stages, where owing to the lack of foragers from Hive 1, we only had 11. All sampled bees were placed directly in liquid nitrogen to prevent changes in the metabolic profile. They were then decapitated in the laboratory so that the head and body of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the stinger apparatus were gently pulled using tweezers, which allowed extraction of the entire gastrointestinal tract. The stinger apparatus and venom sac were carefully excised and the honey crop was removed.
Sample Type:Bee gut

Treatment:

Treatment ID:TR003690
Treatment Summary:On the day of newly emerged workers (day 0), bees were sampled as a baseline for no or low bacterial abundance in the gut. Moreover, bees from each hive were collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples for the in-hive worker phase, defined as workers inside the hives that have a fully developed microbiome, were taken every day from Day 7 to Day 10. For the foraging phase, we differentiated between new and experienced foragers, as these show different behaviour due to different levels of experience.Based on experience, they were either collected one to two days after onset (new foragers) or at least four days after onset (experienced foragers).

Sample Preparation:

Sampleprep ID:SP003688
Sampleprep Summary:In brief, x mg pool gut was mixed with five times the volume (in µL) of acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After centrifugation (2 min, 14000 rpm) 40 µl were used for further derivatization of short chain fatty acids. Each sample was mixed with ACN to a final concentration of 50 %. SCFAs were derivatized with 200 mM 3-nitrophenylhydrazine and 120 mM N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride in pyridine and then diluted in 10 % ACN.

Chromatography:

Chromatography ID:CH004435
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:0-2min: 15% B, 2-17min: 15-50% B, 17-17.1min: 50-100% B, 17.1-18min: 100% B, 18-18.1min: 100-15% B, 18.1-21min: 15% B
Flow Rate:0.35 mL/min
Solvent A:100% water; 0.01% formic acid
Solvent B:100% acetonitrile; 0.01% formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN005839
Analysis Type:MS
Chromatography ID:CH004435
Num Factors:7
Num Metabolites:7
Units:µMol
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