Summary of Study ST003553

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002188. The data can be accessed directly via it's Project DOI: 10.21228/M8SV5J This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003553
Study TitleInvestigation of age-dependent changes in the gut of honeybee workers using targeted metabolomics for amino acid and biogenic amine determination
Study SummaryIn the honeybee guts we measured the concentrations of 20 amino acids and 6 biogenic amines. Tryptophan and its downstream metabolite kynurenine followed the same trends across the worker development, with a significant decrease in their concentrations from day 3 onwards. Serotonin, on the other hand, was significantly increased from day 1. Tyramine and octopamine showed significant increases in concentrations (octopamine for all age groups apart from Day 1; tyramine for all age groups), while their precursor tyrosine showed significantly decreased concentrations for all age groups except experienced foragers.
Institute
Helmholtz Centre for Environmental Research
DepartmentMolecular Systems Biology
Last NameEngelmann
First NameBeatrice
AddressPermoserstraße 15, Leipzipg, Saxony, 03418, Germany
Emailbeatrice.engelmann@ufz.de
Phone004934160251099
Submit Date2024-10-28
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-11-27
Release Version1
Beatrice Engelmann Beatrice Engelmann
https://dx.doi.org/10.21228/M8SV5J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002188
Project DOI:doi: 10.21228/M8SV5J
Project Title:Comprehension of the age-dependent gut and brain interaction of honeybee workers by integration of multi omics approaches
Project Summary:In honeybees, division of labour is a key feature, with age-related behavioural transitions being closely associated with molecular changes in the brain, gut, and microbiota. In this study, to investigate these molecular changes and thus better understand their contribution to behavioural responses and modulation, we analysed the global metabolomic shifts in honeybee workers and their microbiota throughout their lives. Overall, our findings provide new insights toward developing potential biomarkers for evaluation of different functional changes related to various environmental stressors.
Institute:Helmholtz Centre for Environmental Research
Department:Molecular Systems Biology
Last Name:Engelmann
First Name:Beatrice
Address:Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
Email:beatrice.engelmann@ufz.de
Phone:004934160251099

Subject:

Subject ID:SU003682
Subject Type:Insect
Subject Species:Apis mellifera
Taxonomy ID:7460
Species Group:Insects

Factors:

Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)

mb_sample_id local_sample_id life stage age of workers in days
SA388113H2_D1_3_dilutedestablishment 1
SA388114H1_D1_3_dilutedestablishment 1
SA388115H1_D1_4_dilutedestablishment 1
SA388116H1_D1_5_dilutedestablishment 1
SA388117H2_D1_1establishment 1
SA388118H2_D1_2establishment 1
SA388119H2_D1_3establishment 1
SA388120H2_D1_4establishment 1
SA388121H2_D1_5establishment 1
SA388122H2_D1_1_dilutedestablishment 1
SA388123H2_D1_2_dilutedestablishment 1
SA388124H2_D1_5_dilutedestablishment 1
SA388125H1_D1_1_dilutedestablishment 1
SA388126H3_D1_1establishment 1
SA388127H3_D1_2establishment 1
SA388128H3_D1_3establishment 1
SA388129H3_D1_4establishment 1
SA388130H3_D1_5establishment 1
SA388131H3_D1_1_dilutedestablishment 1
SA388132H3_D1_2_dilutedestablishment 1
SA388133H3_D1_3_dilutedestablishment 1
SA388134H3_D1_4_dilutedestablishment 1
SA388135H3_D1_5_dilutedestablishment 1
SA388136H1_D1_2_dilutedestablishment 1
SA388137H2_D1_4_dilutedestablishment 1
SA388138H1_D1_5establishment 1
SA388139H1_D1_4establishment 1
SA388140H1_D1_3establishment 1
SA388141H1_D1_1establishment 1
SA388142H1_D1_2establishment 1
SA388143H2_D3_3establishment 3
SA388144H2_D3_2establishment 3
SA388145H2_D3_1establishment 3
SA388146H2_D3_3_dilutedestablishment 3
SA388147H3_D3_1_dilutedestablishment 3
SA388148H2_D3_4establishment 3
SA388149H2_D3_5establishment 3
SA388150H3_D3_2_dilutedestablishment 3
SA388151H3_D3_3_dilutedestablishment 3
SA388152H3_D3_4_dilutedestablishment 3
SA388153H3_D3_5_dilutedestablishment 3
SA388154H1_D3_2establishment 3
SA388155H1_D3_1establishment 3
SA388156H2_D3_2_dilutedestablishment 3
SA388157H2_D3_5_dilutedestablishment 3
SA388158H1_D3_2_dilutedestablishment 3
SA388159H2_D3_1_dilutedestablishment 3
SA388160H1_D3_3establishment 3
SA388161H2_D3_4_dilutedestablishment 3
SA388162H3_D3_5establishment 3
SA388163H3_D3_4establishment 3
SA388164H3_D3_3establishment 3
SA388165H3_D3_2establishment 3
SA388166H3_D3_1establishment 3
SA388167H1_D3_1_dilutedestablishment 3
SA388168H1_D3_3_dilutedestablishment 3
SA388169H1_D3_4_dilutedestablishment 3
SA388170H1_D3_5_dilutedestablishment 3
SA388171H1_D3_4establishment 3
SA388172H1_D3_5establishment 3
SA388173H2_D5_1_dilutedestablishment 5
SA388174H2_D5_5_dilutedestablishment 5
SA388175H3_D5_5establishment 5
SA388176H2_D5_4_dilutedestablishment 5
SA388177H2_D5_3_dilutedestablishment 5
SA388178H2_D5_2_dilutedestablishment 5
SA388179H2_D5_4establishment 5
SA388180H1_D5_1establishment 5
SA388181H2_D5_5establishment 5
SA388182H3_D5_4establishment 5
SA388183H2_D5_3establishment 5
SA388184H3_D5_1_dilutedestablishment 5
SA388185H3_D5_3establishment 5
SA388186H3_D5_2establishment 5
SA388187H3_D5_1establishment 5
SA388188H1_D5_1_dilutedestablishment 5
SA388189H1_D5_2_dilutedestablishment 5
SA388190H1_D5_3_dilutedestablishment 5
SA388191H1_D5_4_dilutedestablishment 5
SA388192H1_D5_5_dilutedestablishment 5
SA388193H3_D5_5_dilutedestablishment 5
SA388194H3_D5_4_dilutedestablishment 5
SA388195H3_D5_3_dilutedestablishment 5
SA388196H3_D5_2_dilutedestablishment 5
SA388197H1_D5_2establishment 5
SA388198H2_D5_2establishment 5
SA388199H1_D5_5establishment 5
SA388200H1_D5_4establishment 5
SA388201H1_D5_3establishment 5
SA388202H2_D5_1establishment 5
SA388203H3_EF_4experienced_forager >12
SA388204H3_EF_3experienced_forager >12
SA388205H3_EF_2experienced_forager >12
SA388206H3_EF_1experienced_forager >12
SA388207H2_EF_1_dilutedexperienced_forager >12
SA388208H2_EF_2_dilutedexperienced_forager >12
SA388209H2_EF_3_dilutedexperienced_forager >12
SA388210H2_EF_4_dilutedexperienced_forager >12
SA388211H2_EF_5_dilutedexperienced_forager >12
SA388212H3_EF_5experienced_forager >12
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Collection:

Collection ID:CO003675
Collection Summary:Workers were pooled to obtain a final sample number of n = 5. The brain(s) and gut(s) from the same workers were assigned the same sample names. In total, we had 15 samples from each age group, with the exception of the foraging stages, where owing to the lack of foragers from Hive 1, we only had 11. All sampled bees were placed directly in liquid nitrogen to prevent changes in the metabolic profile. They were then decapitated in the laboratory so that the head and body of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the stinger apparatus were gently pulled using tweezers, which allowed extraction of the entire gastrointestinal tract. The stinger apparatus and venom sac were carefully excised and the honey crop was removed.
Sample Type:Bee gut

Treatment:

Treatment ID:TR003691
Treatment Summary:On the day of newly emerged workers (day 0), bees were samples as a baseline for no or low bacterial abundance in the gut. Moreover, bees from each hive were collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples for the in-hive worker phase, defined as workers inside the hives that have a fully developed microbiome, were taken every day from Day 7 to Day 10. For the foraging phase, we differentiated between new and experienced foragers, as these show different behaviour due to different levels of experience.Based on experience, they were either collected one to two days after onset (new foragers) or at least four days after onset (experienced foragers).

Sample Preparation:

Sampleprep ID:SP003689
Sampleprep Summary:In brief, x mg pool gut was mixed with five times the volume (in µL) of acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After Centrifugation (2 min, 14000 rpm), 10 µL were used for amino acid derivatization. First, the supernatant was evaporated to dryness (SpeedVac, Eppendorf), resuspended in 50 µL of 5% phenyl isothiocyanate (PITC) in ethanol: H2O: pyridine (1:1:1, v/v/v), and incubated for 25 min at RT. Subsequently, the samples were dried to remove excess PITC and resuspended in 10 µL 5 mM ammonium acetate in methanol. After incubation (10 min at 14,000 rpm) 90 µL of H2O: ACN + 0.2% formic acid were added. Each derivative was measured in an undiluted and diluted (1:25) manner.

Chromatography:

Chromatography ID:CH004436
Instrument Name:Waters Acquity
Column Name:Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um)
Column Temperature:50
Flow Gradient:0-0.5 min at 0% B, 0.5-4 min 0-70% B, 4-5.3 min 70% B, 5.3-5.4 min 70-0% B, 5.4-7.3 min 0% B
Flow Rate:0.5 ml/min
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile; 0.2% formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN005840
Analysis Type:MS
Chromatography ID:CH004436
Num Factors:7
Num Metabolites:23
Units:µMol
  
Analysis ID:AN005841
Analysis Type:MS
Chromatography ID:CH004436
Num Factors:7
Num Metabolites:3
Units:µMol
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