Summary of Study ST003612
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002232. The data can be accessed directly via it's Project DOI: 10.21228/M8424B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003612 |
| Study Title | Candida auris planktonic and dispersed cells Chromatographic analysis using GC-MS |
| Study Summary | The emerging Candida auris (C. auris) has caused several outbreaks globally. While several studies explored the resistant biofilm formed by C. auris, little is known regarding the cells dispersed following biofilm formation. Herein, I investigated and characterized the cells dispersed from C. auris biofilms. Cells dispersed from biofilm developed in 96 well plate were isolated and counted. GC-MS analysis indicated that dispersed cells exhibit altered metabolic profile that enhance cells survivability under stress and nutrient limit condition. The presented study is the first to explore C. auris dispersed cells and indicated that they are not able to revert to the planktonic mode once released from the biofilm. |
| Institute | National Research Centre, Dokki, Egypt |
| Last Name | Fayed |
| First Name | Bahgat |
| Address | Dokki |
| bm.ezzat@nrc.sci.eg | |
| Phone | +201098292083 |
| Submit Date | 2024-11-23 |
| Num Groups | two |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wmf (Windows Metafile Format) |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-12-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002232 |
| Project DOI: | doi: 10.21228/M8424B |
| Project Title: | Candida auris planktonic and dispersed cells Chromatographic analysis using GC-MS |
| Project Summary: | The emerging Candida auris (C. auris) has caused several outbreaks globally. While several studies explored the resistant biofilm formed by C. auris, little is known regarding the cells dispersed following biofilm formation. Herein, I investigated and characterized the cells dispersed from C. auris biofilms. Cells dispersed from biofilm developed in 96 well plate were isolated and counted. GC-MS analysis indicated that dispersed cells exhibit altered metabolic profile that enhance cells survivability under stress and nutrient limit condition. The presented study is the first to explore C. auris dispersed cells and indicated that they are not able to revert to the planktonic mode once released from the biofilm. |
| Institute: | National Research Centre, Dokki, Egypt |
| Last Name: | Fayed |
| First Name: | Bahgat |
| Address: | Dokki, Giza, Cairo, 11433, Egypt |
| Email: | bm.ezzat@nrc.sci.eg |
| Phone: | +201098292083 |
Subject:
| Subject ID: | SU003741 |
| Subject Type: | Yeast |
| Subject Species: | Candida auris |
Factors:
Subject type: Yeast; Subject species: Candida auris (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Geno type |
|---|---|---|---|
| SA392800 | sample tested | Candida auris dispersed cells | Wild |
| SA392801 | Control | Candida auris planktonic cells | Wild |
| Showing results 1 to 2 of 2 |
Collection:
| Collection ID: | CO003734 |
| Collection Summary: | C. auris dispersed cell were allowed to grow in 250-mL Erlenmeyer flasks containing 50 mL of SD broth media for 3 days at 30°C. Following incubation, the supernatant was separated by centrifugation for 20 min at 4000 rpm. The resulting supernatant was extracted with 100 mL ethyl acetate. |
| Sample Type: | Candida auris |
Treatment:
| Treatment ID: | TR003750 |
| Treatment Summary: | Isolation of cells dispersed from C. auris biofilm C. auris biofilms were developed by loading 1×106 cells/ml in RPMI-1640 supplemented with 2% glucose and then incubating for 24 h at 37°C in 96-well flat-bottomed microplates [17]. Following incubation, the supernatant containing floating cells was removed, the formed biofilm was washed 3 times with PBS, fresh media was added, and the biofilm was incubated for an additional 24 h under the same conditions. The next day, the cells dispersed from the biofilm in the upper supernatant were collected, counted by a hemocytometer and maintained in SD media or stored in 30% glycerol for further use. |
Sample Preparation:
| Sampleprep ID: | SP003748 |
| Sampleprep Summary: | C. auris dispersed cell were allowed to grow in 250-mL Erlenmeyer flasks containing 50 mL of SD broth media for 3 days at 30°C. Following incubation, the supernatant was separated by centrifugation for 20 min at 4000 rpm. The resulting supernatant was extracted with 100 mL ethyl acetate. After the extraction step, the organic phases were dried on anhydrous MgSO4, filtered, and concentrated under reduced pressure. The parent C. auris planktonic cells served as control. |
Chromatography:
| Chromatography ID: | CH004512 |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent DB5-MS (15m x 0.25mm, 0.25um) |
| Column Temperature: | 300°C |
| Flow Gradient: | nothing |
| Flow Rate: | nothing |
| Solvent A: | nothing |
| Solvent B: | nothing |
| Chromatography Type: | GC |
Analysis:
| Analysis ID: | AN005937 |
| Analysis Type: | MS |
| Chromatography ID: | CH004512 |
| Num Factors: | 2 |
| Num Metabolites: | 83 |
| Units: | Area Pct |
