Summary of Study ST003650

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002260. The data can be accessed directly via it's Project DOI: 10.21228/M8H254 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003650
Study TitleInvestigation of the effects of 3-hydroxybutyrate on myoblast proliferation and differentiation using NMR-based metabolomics
Study SummaryThis study aims to further elucidate the molecular mechanisms by which 3-HB promotes the proliferation and differentiation of C2C12 myoblasts. By performing NMR-based metabolomic analysis on myoblasts, we explored the dual roles of 3-HB functioning as a metabolic substrate and a signaling molecule in myoblasts, and uncovered the underlying molecular mechanisms. Our results may significantly contribute to the development of 3-HB as a potential therapeutic agent against skeletal muscle atrophy.
Institute
Xiamen University
Last NameQiu
First NameXu
AddressXiamen University
Emailqiuxu@stu.xmu.edu.cn
Phone13395036603
Submit Date2025-01-01
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2025-01-26
Release Version1
Xu Qiu Xu Qiu
https://dx.doi.org/10.21228/M8H254
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002260
Project DOI:doi: 10.21228/M8H254
Project Title:3-Hydroxybutyrate promotes myoblast proliferation and differentiation
Project Summary:Muscle wasting is a major clinical challenge in various diseases and physiological states, as the loss of skeletal muscle mass adversely affects patient outcomes. This study elucidates the role of the endogenously supplied metabolite 3-hydroxybutyrate (3-HB) in promoting proliferation and differentiation of C2C12 myoblasts through nuclear magnetic resonance (NMR)-based metabolomic analysis.
Institute:Xiamen University
Last Name:Qiu
First Name:Xu
Address:Xiamen University
Email:qiuxu@stu.xmu.edu.cn
Phone:13395036603

Subject:

Subject ID:SU003780
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Factors
SA398368CK3Treatment 5 mM-3HB
SA398369CK10Treatment 5 mM-3HB
SA398370CK9Treatment 5 mM-3HB
SA398371CK8Treatment 5 mM-3HB
SA398372CK7Treatment 5 mM-3HB
SA398373CK6Treatment 5 mM-3HB
SA398374CK5Treatment 5 mM-3HB
SA398375CK4Treatment 5 mM-3HB
SA398376CK1Treatment 5 mM-3HB
SA398377CK2Treatment 5 mM-3HB
SA398378C2Treatment Control
SA398379C1Treatment Control
SA398380C10Treatment Control
SA398381C8Treatment Control
SA398382C7Treatment Control
SA398383C6Treatment Control
SA398384C5Treatment Control
SA398385C4Treatment Control
SA398386C3Treatment Control
SA398387C9Treatment Control
SA398388L7Treatment Low glucose
SA398389L10Treatment Low glucose
SA398390L9Treatment Low glucose
SA398391L8Treatment Low glucose
SA398392L6Treatment Low glucose
SA398393L5Treatment Low glucose
SA398394L4Treatment Low glucose
SA398395L3Treatment Low glucose
SA398396L2Treatment Low glucose
SA398397L1Treatment Low glucose
SA398398LK1Treatment Low glucose+3HB
SA398399LK2Treatment Low glucose+3HB
SA398400LK3Treatment Low glucose+3HB
SA398401LK4Treatment Low glucose+3HB
SA398402LK5Treatment Low glucose+3HB
SA398403LK6Treatment Low glucose+3HB
SA398404LK7Treatment Low glucose+3HB
SA398405LK8Treatment Low glucose+3HB
SA398406LK9Treatment Low glucose+3HB
SA398407LK10Treatment Low glucose+3HB
Showing results 1 to 40 of 40

Collection:

Collection ID:CO003773
Collection Summary:Cells were rinsed three times with precooled phosphate-buffered saline (PBS) to remove residual medium. To stop metabolic activity, 3 mL of pre-cooled (-80°C) methanol was added to the cells. The cells were then scraped with a cell scraper and collected in 15 mL centrifuge tubes. A two-phase extraction was performed using a methanol: chloroform: water mixture in a ratio of 4:4:2.85 (v/v) to extract water-soluble metabolites from the cells. The aqueous phase was collected and lyophilized.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR003789
Treatment Summary:C2C12 myoblasts were cultured in Dulbecco's modified Eagle's medium (DMEM, Corning, USA) supplemented with 10% fetal bovine serum (FBS, Corning, USA) and 1% penicillin-streptomycin (PS, Hyclone, USA) for cell growth and maintenance. The medium was changed periodically to ensure optimal cell health. To prepare low-glucose DMEM (0.5 g/L D-glucose, without sodium pyruvate), we mixed standard DMEM (4.5 g/L D-glucose, without sodium pyruvate, Corning, USA) with sugar-free DMEM (without sodium pyruvate, Corning, USA) in a ratio of 1:8. This was followed by the addition of 10% FBS to create the conditions necessary to model myoblasts and myotubes, respectively, under low-glucose conditions. Sodium (R)-3-hydroxybutyrate (Sigma-Aldrich, China) was added to the medium.

Sample Preparation:

Sampleprep ID:SP003787
Sampleprep Summary:The resulting metabolite powder was dissolved in 550 μL of NMR buffer (prepared in D2O, 50 mM PO₄³-, 0.05 mM TSP, pH 7.4) and transferred to a 5 mm NMR tube for acquiring the NMR spectrum.

Analysis:

Analysis ID:AN005996
Analysis Type:NMR
Results File:ST003650_AN005996_Results.txt
Units:area under the curve

NMR:

NMR ID:NM000301
Analysis ID:AN005996
Instrument Name:Bruker Avance III HD 850 MHz
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Standard Concentration:0.05 mM TSP
Spectrometer Frequency:850 MHz
NMR Solvent:H2O+D2O
NMR Tube Size:5 mm
Pulse Sequence:noesygppr1d [(RD)-90°-t1-90°-τm-90°-ACQ]
Receiver Gain:144
Offset Frequency:15.02 ppm
Temperature:25°C
Number Of Scans:32
Dummy Scans:4
Acquisition Time:2 s
Spectral Width:20 ppm
Num Data Points Acquired:64 K
Line Broadening:0.3 Hz
Baseline Correction Method:Auto-baseline correction of integral by abs
Chemical Shift Ref Std:TSP (0.000 ppm)
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