Summary of Study ST003729

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002315. The data can be accessed directly via it's Project DOI: 10.21228/M8D26K This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003729
Study TitleHoney bee egg composition changes seasonally and after acute maternal virus infection.
Study TypeMetabolomics and lipidomics
Study SummaryHoney bee (Apis mellifera) colonies depend on the reproductive output of their queens, which in turn is contingent on the care provided by worker bees. In this study, we collected eggs from honey bee queens in natural field conditions and found that egg collection date strongly influenced egg composition, likely reflecting seasonal variations in pollen resources. These findings highlight that while viral infections can induce transgenerational effects on egg proteomes under short-term experimental conditions, such effects are less apparent in natural settings and can be overshadowed by seasonal and other ecological factors.
Institute
University of British Columbia
DepartmentBiochemistry and Molecular Biology, Life Sciences Institute
LaboratoryFoster Lab
Last NameAlcazar Magana
First NameArmando
Address2350 Health Sciences Mall
Emailarmando.alcazarmagana@ubc.ca
Phone5416097172
Submit Date2024-09-26
Raw Data AvailableYes
Raw Data File Type(s)mzML, d
Analysis Type DetailLC-MS
Release Date2025-03-05
Release Version1
Armando Alcazar Magana Armando Alcazar Magana
https://dx.doi.org/10.21228/M8D26K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002315
Project DOI:doi: 10.21228/M8D26K
Project Title:Honey bee egg composition changes seasonally
Project Summary:Here we investigate bee eggs sampled from a field survey to see if the lipid and metabolite composition differs as a result of naturally-occurring queen virus infections.
Institute:University of British Columbia
Department:Biochemistry and Molecular Biology, Life Sciences Institute
Laboratory:Foster Lab
Last Name:Alcazar Magana
First Name:Armando
Address:2350 Health Sciences Mall, Vancouver, BC, V6T1Z3, Canada
Email:armando.alcazarmagana@ubc.ca
Phone:5416097172

Subject:

Subject ID:SU003861
Subject Type:Insect
Subject Species:Apis mellifera
Taxonomy ID:7460
Gender:Not applicable

Factors:

Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA40729028Beekeeper1-Jun15
SA40729133Beekeeper1-Jun15
SA40729232Beekeeper1-Jun15
SA40729331Beekeeper1-Jun15
SA40729430Beekeeper1-Jun15
SA40729529Beekeeper1-Jun15
SA4072962Beekeeper1-May14
SA4072971Beekeeper1-May14
SA40729810Beekeeper1-May14
SA4072999Beekeeper1-May14
SA4073003Beekeeper1-May14
SA4073014Beekeeper1-May14
SA4073025Beekeeper1-May14
SA4073036Beekeeper1-May14
SA4073047Beekeeper1-May14
SA4073058Beekeeper1-May14
SA40730639Beekeeper2-Jun16
SA40730740Beekeeper2-Jun16
SA40730837Beekeeper2-Jun16
SA40730941Beekeeper2-Jun16
SA40731042Beekeeper2-Jun16
SA40731143Beekeeper2-Jun16
SA40731245Beekeeper2-Jun16
SA40731346Beekeeper2-Jun16
SA40731447Beekeeper2-Jun16
SA40731538Beekeeper2-Jun16
SA40731644Beekeeper2-Jun16
SA40731736Beekeeper2-Jun16
SA40731834Beekeeper2-Jun16
SA40731935Beekeeper2-Jun16
SA40732017Beekeeper2-May15
SA40732111Beekeeper2-May15
SA40732212Beekeeper2-May15
SA40732315Beekeeper2-May15
SA40732413Beekeeper2-May15
SA40732519Beekeeper2-May15
SA40732618Beekeeper2-May15
SA40732720Beekeeper2-May15
SA40732821Beekeeper2-May15
SA40732975Beekeeper3-Jun27
SA40733083Beekeeper3-Jun27
SA40733176Beekeeper3-Jun27
SA40733277Beekeeper3-Jun27
SA40733378Beekeeper3-Jun27
SA40733479Beekeeper3-Jun27
SA40733580Beekeeper3-Jun27
SA40733681Beekeeper3-Jun27
SA40733782Beekeeper3-Jun27
SA40733889Beekeeper3-Jun27
SA40733984Beekeeper3-Jun27
SA40734085Beekeeper3-Jun27
SA40734186Beekeeper3-Jun27
SA40734287Beekeeper3-Jun27
SA40734388Beekeeper3-Jun27
SA40734490Beekeeper3-Jun27
SA40734592Beekeeper3-Jun27
SA40734693Beekeeper3-Jun27
SA40734794Beekeeper3-Jun27
SA40734873Beekeeper3-Jun27
SA40734974Beekeeper3-Jun27
SA40735056Beekeeper3-Jun27
SA40735172Beekeeper3-Jun27
SA40735259Beekeeper3-Jun27
SA40735348Beekeeper3-Jun27
SA40735449Beekeeper3-Jun27
SA40735550Beekeeper3-Jun27
SA40735652Beekeeper3-Jun27
SA40735753Beekeeper3-Jun27
SA40735854Beekeeper3-Jun27
SA40735955Beekeeper3-Jun27
SA40736057Beekeeper3-Jun27
SA40736158Beekeeper3-Jun27
SA40736260Beekeeper3-Jun27
SA40736371Beekeeper3-Jun27
SA40736461Beekeeper3-Jun27
SA40736562Beekeeper3-Jun27
SA40736663Beekeeper3-Jun27
SA40736764Beekeeper3-Jun27
SA40736865Beekeeper3-Jun27
SA40736966Beekeeper3-Jun27
SA40737067Beekeeper3-Jun27
SA40737168Beekeeper3-Jun27
SA40737269Beekeeper3-Jun27
SA40737351Beekeeper3-Jun27
Showing results 1 to 84 of 84

Collection:

Collection ID:CO003854
Collection Summary:Honey bee queens and their eggs were sampled from colonies in the field in 2021. Samples were collected twice from the two British Columbia beekeepers (in Grand Forks and Vernon), first on May 14 & 15 and then again on June 15 & 16, and collected from a Lethbridge, Alberta, beekeeper on June 27 & 28. At each time point, 10 eggs still standing on end (not fallen onto their side, which is indicative of older eggs close to hatching) were collected using a Chinese grafting tool and transferred to Eppendorf tubes. All samples were collected directly onto dry-ice and subsequently stored at -70 °C until processing.
Sample Type:Eggs
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003870
Treatment Summary:No treatment (collection time is of interest and no treatment was administered)

Sample Preparation:

Sampleprep ID:SP003867
Sampleprep Summary:Eppendorf tubes containing 10 eggs each were centrifuged for 1 min at 14,000 rcf immediately upon removal from the freezer freezer followed by a two-phase extraction protocol adapted from Matyash et al. (2008) with some modifications. Briefly, 100 μL of methanol extraction solvent (75% methanol:water, made with 0.01% BHT-methanol and containing standards of methionine-d3, caffeine-13C3, and ferulic acid-d3 each at 1 ppm and SPLASH LIPIDOMIX (Avanti Polar Lipids, Alabama, USA) at 5 ppm) was added. The eggs were then ground with a mini-pestle to lyse the eggs. The tubes were thoroughly vortexed, and samples were transferred to a 2 mL screw cap tube homogenizer tube. Another 100 μL of extraction solvent was used to rinse the original tube and was also transferred to the homogenization tube to increase yields. 2 ceramic beads were then added and samples were homogenized three times for 30 seconds at 6500 rpm as described for proteomics sample processing. 500 μL of methyl tert-butyl ether was then added and samples were incubated for 1 hour at room temperature while shaking at 1000 rpm. The mixture was transferred to a new tube and centrifuged at 14,000 rcf for 10 min. 600 μL was transferred to a new tube to which 107 μL of water was added (to a final additional concentration of 15%) before incubating again at room temperature for 10 minutes while shaking at 700 rpm. The samples were then centrifuged for 15 min at 14000 rcf at 4C. 300 μL of the upper fraction containing the non-polar (lipid) compounds were transferred to a new tube, while 400 μL of the lower fraction containing the polar (metabolite) compounds were transferred to a different tube. The polar fraction was dried (SpeedVac, Eppendorf) immediately and stored at -70 °C until mass spectrometry analysis. The non-polar fraction was stored in solution at -70 °C and dried immediately before analysis to prevent oxidation.
Processing Storage Conditions:On ice

Chromatography:

Chromatography ID:CH004646
Chromatography Summary:Metabolomics with Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm, GL Sciences)
Instrument Name:Thermo Vanquish
Column Name:GL Sciences Inertsil Ph-3 UHPLC (150 x 2.1mm, 2um)
Column Temperature:55
Flow Gradient:0 min (5% B), 0–1 min (5% B), 1–8 min (35% B), 8–10.5 min (99% B), 10.5–14 min (99% B), 14–14.5 min (5% B), and 14.5–18 min (5% B)
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid; 10 mM ammonium acetate; 5 µM medronic acid
Solvent B:100% methanol; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH004647
Chromatography Summary:Lipidomics with Acquity CSH column
Instrument Name:Elute LC
Column Name:Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:65
Flow Gradient:0 min 15% B; 0–2 min 30% B; 2–2.5 min 50% B; 2.5–12 min 80% B; 12–12.5 min 99% B; 12.5–13.5 min 99% B; 13.5–13.7 min 15% B; 13.7-17 min 15% B.
Flow Rate:0.5 mL/min
Solvent A:60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN006117
Analysis Type:MS
Chromatography ID:CH004646
Num Factors:5
Num Metabolites:86
Rt Units:Minutes
Units:Relative Abundance
  
Analysis ID:AN006118
Analysis Type:MS
Chromatography ID:CH004646
Num Factors:5
Num Metabolites:87
Rt Units:Minutes
Units:Relative Abundance
  
Analysis ID:AN006119
Analysis Type:MS
Chromatography ID:CH004647
Num Factors:5
Num Metabolites:72
Rt Units:Minutes
Units:Relative Abundance
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