Summary of Study ST003739
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002325. The data can be accessed directly via it's Project DOI: 10.21228/M83J9R This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003739 |
| Study Title | Serum neurotransmitter targeted metabolome in sleep-deprived (SD) mice |
| Study Summary | Sleep disturbances associate with pathogenesis of numerous chronic disorders, including chronic gastrointestinal diseases. However, the mechanism that transmits sleep disturbance-induced aberrant neural signaling from the brain to the gut remains elusive. We show that acute sleep deprivation (SD) impairs intestinal stem cell function, leading to shortening of crypt-villus architecture and Paneth cell loss. We identified dorsal motor nucleus of vagus (DMV) as the SD-sensitive central nervous system center that transmits sleep effects to the gut. SD aberrantly activates DMV neurons, driving excessive acetylcholine release from the vagus nerve into the gut. Acetylcholine triggers 5-hydroxytryptamine (5-HT) release by enterochromaffin cells and suppresses its reuptake via muscarinic receptors, thereby causing a spike in 5-HT levels. Elevated 5-HT induces excessive oxidative stress in intestinal stem cells through its receptor HTR4, promoting gut pathologies. Overall, we reveal SD-responsive neural circuit that controls intestinal stem cells and identify therapeutic strategies for mitigating SD-related gut diseases. |
| Institute | China Agricultural University |
| Last Name | Zhang |
| First Name | Mingxin |
| Address | 2 West Yuanmingyuan Road, Beijing, Beijing, 100193, China |
| zmx15310686122@163.com | |
| Phone | 18800152903 |
| Submit Date | 2025-02-14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2026-01-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002325 |
| Project DOI: | doi: 10.21228/M83J9R |
| Project Title: | Serum neurotransmitter targeted metabolome in sleep-deprived mice |
| Project Summary: | This project investigates the changes in serum neurotransmitter levels before and after 2 days of sleep deprivation in mice, exploring the alterations in serum neurotransmitter release caused by sleep deprivation. Metabolomic results indicate that in mice subjected to 2 days of sleep deprivation, serum neurotransmitters such as 5-HTP, Kyn, and KynA were significantly reduced, while Ach, Gln, Trp, and 5-HT were significantly increased. |
| Institute: | China Agriculture University |
| Last Name: | Zhang |
| First Name: | Mingxin |
| Address: | 2 West Yuanmingyuan Road, Beijing, Beijing, 100193, China |
| Email: | zmx15310686122@163.com |
| Phone: | 18800152903 |
Subject:
| Subject ID: | SU003871 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA407853 | SD0d_1 | Ctrl |
| SA407854 | SD0d_2 | Ctrl |
| SA407855 | SD0d_3 | Ctrl |
| SA407856 | SD0d_4 | Ctrl |
| SA407857 | SD0d_5 | Ctrl |
| SA407858 | SD2d_1 | SD2d |
| SA407859 | SD2d_2 | SD2d |
| SA407860 | SD2d_3 | SD2d |
| SA407861 | SD2d_4 | SD2d |
| SA407862 | SD2d_5 | SD2d |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003864 |
| Collection Summary: | Serum samples were obtained immediately after mice were subjected to 48 hours of continuous sleep deprivation(SD). Fresh blood from mice was collected via cardiac puncture before and after sleep deprivation. After allowing the blood to stand for 1 hour, it was centrifuged at 3000g for 10 minutes to obtain 400 µL of serum, which was then stored at -80°C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR003880 |
| Treatment Summary: | The samples (100 μL for each sample) were homogenized with 200 μL of acetonitrile (80%) which contained mixed internal standards and centrifuged at 12,000 rpm for 10 min. Finally, the supernatant (2 μL) was injected into the LC-MS/MS system for analysis. |
Sample Preparation:
| Sampleprep ID: | SP003877 |
| Sampleprep Summary: | The stock solution of individual neurotransmitter was mixed and prepared in neurotransmitter-free matrix to obtain a series of neurotransmitter calibrators. Certain concentrations of L-Tyrosine-d4 was mixed as Internal Standard (IS). The stock solution of all of these and working solution were stored in refrigerator of -20°C |
Combined analysis:
| Analysis ID | AN006139 |
|---|---|
| Chromatography ID | CH004663 |
| MS ID | MS005845 |
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | AB Sciex QTRAP 6500+ |
| Column | Waters XSelect HSS T3 XP (150 x 3mm,2.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | POSITIVE |
| Units | ng/ml |
Chromatography:
| Chromatography ID: | CH004663 |
| Instrument Name: | AB Sciex QTRAP 6500+ |
| Column Name: | Waters XSelect HSS T3 XP (150 x 3mm,2.5um) |
| Column Temperature: | 45°C |
| Flow Gradient: | initial 5% B, 2min; 5-40% B, 4min; 40-70% B, 7 min; 70-100% B, 7.5min; 100% B, 9min; 100-5% B, 9.1min; 5% B, 11min. |
| Flow Rate: | 0.30 mL/min |
| Solvent A: | 100% Water; 0.1% Formic acid; 5mM Ammonium acetate |
| Solvent B: | 100% Acetonitrile; 0.1% Formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS005845 |
| Analysis ID: | AN006139 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Curtain Gas：35psi； Collision Gas：Medium；IonSpray Voltage：4500V； Temperature：550℃； Ion Source Gas 1：60； Ion Source Gas 2：60 |
| Ion Mode: | POSITIVE |
