Summary of Study ST003746
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002332. The data can be accessed directly via it's Project DOI: 10.21228/M86C29 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003746 |
| Study Title | Serum hormone-targeted metabolome of sleep-deprived mice |
| Study Summary | Sleep disturbances associate with pathogenesis of numerous chronic disorders, including chronic gastrointestinal diseases. However, the mechanism that transmits sleep disturbance-induced aberrant neural signaling from the brain to the gut remains elusive. We show that acute sleep deprivation (SD) impairs intestinal stem cell function, leading to shortening of crypt-villus architecture and Paneth cell loss. We identified dorsal motor nucleus of vagus (DMV) as the SD-sensitive central nervous system center that transmits sleep effects to the gut. SD aberrantly activates DMV neurons, driving excessive acetylcholine release from the vagus nerve into the gut. Acetylcholine triggers 5-hydroxytryptamine (5-HT) release by enterochromaffin cells and suppresses its reuptake via muscarinic receptors, thereby causing a spike in 5-HT levels. Elevated 5-HT induces excessive oxidative stress in intestinal stem cells through its receptor HTR4, promoting gut pathologies. Overall, we reveal SD-responsive neural circuit that controls intestinal stem cells and identify therapeutic strategies for mitigating SD-related gut diseases. |
| Institute | China Agricultural University |
| Last Name | Zhang |
| First Name | Mingxin |
| Address | 2 West Yuanmingyuan Road, Beijing, Beijing, 100193, China |
| zmx15310686122@163.com | |
| Phone | 18800152903 |
| Submit Date | 2025-02-14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2026-01-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002332 |
| Project DOI: | doi: 10.21228/M86C29 |
| Project Title: | Serum hormone-targeted metabolome of sleep-deprived mice |
| Project Summary: | This project investigates the changes in serum hormone levels before and after 2 days of sleep deprivation in mice, exploring the alterations in serum hormone release caused by sleep deprivation. Metabolomic results indicate that in mice subjected to 2 days of sleep deprivation, serum hormones such as DHT, Progesterone, and Corticosterone were significantly reduced |
| Institute: | China Agriculture University |
| Last Name: | Zhang |
| First Name: | Mingxin |
| Address: | 2 West Yuanmingyuan Road, Beijing, Beijing, 100193, China |
| Email: | zmx15310686122@163.com |
| Phone: | 18800152903 |
Subject:
| Subject ID: | SU003879 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA408229 | SD0d_1 | Ctrl |
| SA408230 | SD0d_2 | Ctrl |
| SA408231 | SD0d_3 | Ctrl |
| SA408232 | SD0d_4 | Ctrl |
| SA408233 | SD0d_5 | Ctrl |
| SA408234 | SD2d_1 | SD2d |
| SA408235 | SD2d_2 | SD2d |
| SA408236 | SD2d_3 | SD2d |
| SA408237 | SD2d_4 | SD2d |
| SA408238 | SD2d_5 | SD2d |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003872 |
| Collection Summary: | Fresh blood from mice was collected via cardiac puncture before and after 48 hours of continuous sleep deprivation. After allowing the blood to stand for 1 hour, it was centrifuged at 3000g for 10 minutes to obtain serum. A total of 100 μL of serum sample was collected. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR003888 |
| Treatment Summary: | The serum sample was mixed by adding 100 μL of methanol, and then 100 μL of water was added and mixed at 4°C. The samples were centrifuged at 12000 rpm for 15 min. |
Sample Preparation:
| Sampleprep ID: | SP003885 |
| Sampleprep Summary: | The supernatant obtained after centrifugation was removed and slowly passed through the Oasis PRIME HLB column. The column was washed with 5% methanol. Finally, the animal hormones were eluted using 90/10 (v/v) acetonitrile/methanol and collected. The eluate was dried and redissolved before injection for LC-MS analysis. |
Combined analysis:
| Analysis ID | AN006152 |
|---|---|
| Chromatography ID | CH004673 |
| MS ID | MS005857 |
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | AB Sciex QTRAP 6500+ |
| Column | Kinetex C18 column (100 x 2.1 mm, 2.6 μm) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | POSITIVE |
| Units | ng/mL |
Chromatography:
| Chromatography ID: | CH004673 |
| Instrument Name: | AB Sciex QTRAP 6500+ |
| Column Name: | Kinetex C18 column (100 x 2.1 mm, 2.6 μm) |
| Column Temperature: | 45°C |
| Flow Gradient: | initial 5% B, 2min; 5-40% B, 4min; 40-70% B, 7 min; 70-100% B, 7.5min; 100% B, 9min; 100-5% B, 9.1min; 5% B, 11min. |
| Flow Rate: | 0.30 mL/min |
| Solvent A: | 100% Water; 0.1% Formic acid; 5mM Ammonium acetate |
| Solvent B: | 100% Acetonitrile; 0.1% Formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS005857 |
| Analysis ID: | AN006152 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Curtain Gas：35psi； Collision Gas：Medium；IonSpray Voltage：4500V； Temperature：550℃； Ion Source Gas 1：60； Ion Source Gas 2：60 The characteristic ion of each substance is screened by the triple quadrupole, and the signal of the characteristic ion is obtained in the detector No. Intensity (CPS), open the sample mass spectrometry file with SCIEX OS V1.4 software, and perform chromatographic peaks Integration and calibration work, the peak Area (Area) of each chromatographic peak represents the relative quantitative value of the corresponding substance, the most All chromatographic peak area integral data were then exported |
| Ion Mode: | POSITIVE |
