Summary of Study ST003782

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002360. The data can be accessed directly via it's Project DOI: 10.21228/M8KG1C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003782
Study TitleBiomonitoring of (pseudo)pregnancy in giant panda urine using polar metabolomics
Study SummaryThe physiological processes during (pseudo)pregnancy in giant pandas are poorly understood and diagnosis of actual pregnancy is only possible in the final 2-3 weeks of the cycle. Therefore, in this study, urine samples from different phases of the reproductive cycle of giant pandas were analyzed using a polar metabolomics approach. In total, five pregnant and seven pseudopregnant cycles were included. Each cycle was subdivided in six reproductive phases and three samples were selected per phase. The polar metabolomics approach was used to investigate differences in metabolism during the (pseudo)pregnant cycle to gain more information of underlying physiological processes and look for potential (early) pregnancy biomarkers. Statistical analysis revealed significant differences between pregnant and pseudopregnant females in amino acid (e.g. tryptophan) and energy metabolism, specifically during the stages before and around corpus luteum reactivation and embryo implantation.
Institute
Ghent University
DepartmentTranslational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine
LaboratoryLaboratory of Integrative Metabolomics (LIMET)
Last NameCools
First NameTom
AddressSalisburylaan 133 9820 Merelbeke
Emailtom.cools@ugent.be; lynn.vanhaecke@ugent.be; jella.wauters@ugent.be
Phone+32 (0)9 264 74 51
Submit Date2025-02-16
Raw Data AvailableYes
Raw Data File Type(s)mzXML, raw(Thermo)
Analysis Type DetailLC-MS
Release Date2025-08-01
Release Version1
Tom Cools Tom Cools
https://dx.doi.org/10.21228/M8KG1C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002360
Project DOI:doi: 10.21228/M8KG1C
Project Title:Giant panda pregnancy metabolomics studies
Project Summary:Monitoring and detection of pregnancy in giant pandas is hampered by phenomena such as delayed implantation and obligatory pseudopregnancy. Urine samples from different phases of the reproductive cycle of pregnant and pseudopregnant giant pandas were therefore analyzed with two UHPLC-HRMS-based methods: polar metabolomics and steroidomics. Discriminative differences were investigated to comprehense physiological differences and enable early discrimination between the two states.
Institute:Ghent University
Department:Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine
Laboratory:Laboratory of Integrative Metabolomics
Last Name:Cools
First Name:Tom
Address:Salisburylaan 133 9820 Merelbeke
Email:tom.cools@ugent.be; lynn.vanhaecke@ugent.be; jella.wauters@ugent.be
Phone:+32 (0)9 264 74 51

Subject:

Subject ID:SU003916
Subject Type:Mammal
Subject Species:Giant panda (Ailuropoda melanoleuca)

Factors:

Subject type: Mammal; Subject species: Giant panda (Ailuropoda melanoleuca) (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Reproductive cycle Reproductive phase
SA410250Blank_25Acetonitrile Blank Blank
SA410251Blank_19Acetonitrile Blank Blank
SA410252Blank_20Acetonitrile Blank Blank
SA410253Blank_21Acetonitrile Blank Blank
SA410254Blank_22Acetonitrile Blank Blank
SA410255Blank_23Acetonitrile Blank Blank
SA410256Blank_24Acetonitrile Blank Blank
SA410257Blank_26Acetonitrile Blank Blank
SA410258Blank_17Acetonitrile Blank Blank
SA410259Blank_27Acetonitrile Blank Blank
SA410260Blank_28Acetonitrile Blank Blank
SA410261Blank_29Acetonitrile Blank Blank
SA410262Blank_30Acetonitrile Blank Blank
SA410263Blank_31Acetonitrile Blank Blank
SA410264Blank_32Acetonitrile Blank Blank
SA410265Blank_18Acetonitrile Blank Blank
SA410266Blank_16Acetonitrile Blank Blank
SA410267Blank_35Acetonitrile Blank Blank
SA410268Blank_7Acetonitrile Blank Blank
SA410269Blank_1Acetonitrile Blank Blank
SA410270Blank_2Acetonitrile Blank Blank
SA410271Blank_3Acetonitrile Blank Blank
SA410272Blank_4Acetonitrile Blank Blank
SA410273Blank_5Acetonitrile Blank Blank
SA410274Blank_6Acetonitrile Blank Blank
SA410275Blank_8Acetonitrile Blank Blank
SA410276Blank_15Acetonitrile Blank Blank
SA410277Blank_9Acetonitrile Blank Blank
SA410278Blank_10Acetonitrile Blank Blank
SA410279Blank_11Acetonitrile Blank Blank
SA410280Blank_12Acetonitrile Blank Blank
SA410281Blank_13Acetonitrile Blank Blank
SA410282Blank_14Acetonitrile Blank Blank
SA410283Blank_34Acetonitrile Blank Blank
SA410284Blank_33Acetonitrile Blank Blank
SA410285Blank_36Acetonitrile Blank Blank
SA410286Blank_62Acetonitrile Blank Blank
SA410287Blank_56Acetonitrile Blank Blank
SA410288Blank_57Acetonitrile Blank Blank
SA410289Blank_58Acetonitrile Blank Blank
SA410290Blank_59Acetonitrile Blank Blank
SA410291Blank_60Acetonitrile Blank Blank
SA410292Blank_61Acetonitrile Blank Blank
SA410293Blank_63Acetonitrile Blank Blank
SA410294Blank_54Acetonitrile Blank Blank
SA410295Blank_64Acetonitrile Blank Blank
SA410296Blank_65Acetonitrile Blank Blank
SA410297Blank_66Acetonitrile Blank Blank
SA410298Blank_67Acetonitrile Blank Blank
SA410299Blank_68Acetonitrile Blank Blank
SA410300Blank_69Acetonitrile Blank Blank
SA410301Blank_37Acetonitrile Blank Blank
SA410302Blank_55Acetonitrile Blank Blank
SA410303Blank_53Acetonitrile Blank Blank
SA410304Blank_44Acetonitrile Blank Blank
SA410305Blank_38Acetonitrile Blank Blank
SA410306Blank_39Acetonitrile Blank Blank
SA410307Blank_40Acetonitrile Blank Blank
SA410308Blank_41Acetonitrile Blank Blank
SA410309Blank_52Acetonitrile Blank Blank
SA410310Blank_43Acetonitrile Blank Blank
SA410311Blank_42Acetonitrile Blank Blank
SA410312Blank_45Acetonitrile Blank Blank
SA410313Blank_46Acetonitrile Blank Blank
SA410314Blank_47Acetonitrile Blank Blank
SA410315Blank_48Acetonitrile Blank Blank
SA410316Blank_49Acetonitrile Blank Blank
SA410317Blank_50Acetonitrile Blank Blank
SA410318Blank_51Acetonitrile Blank Blank
SA410319MM190114Urine Pregnancy Anestrus
SA410320WW191223Urine Pregnancy Anestrus
SA410321WW191130Urine Pregnancy Anestrus
SA410322WW191101Urine Pregnancy Anestrus
SA410323FFr170115Urine Pregnancy Anestrus
SA410324MM190315Urine Pregnancy Anestrus
SA410325MM190215Urine Pregnancy Anestrus
SA410326FFr170101Urine Pregnancy Anestrus
SA410327HH190101Urine Pregnancy Anestrus
SA410328HH150309Urine Pregnancy Anestrus
SA410329HH190301Urine Pregnancy Anestrus
SA410330FFr170131Urine Pregnancy Anestrus
SA410331HH190201Urine Pregnancy Anestrus
SA410332HH150215Urine Pregnancy Anestrus
SA410333HH150130Urine Pregnancy Anestrus
SA410334MM190422Urine Pregnancy CLD1
SA410335FFr170411Urine Pregnancy CLD1
SA410336WW200206_2Urine Pregnancy CLD1
SA410337HH190428Urine Pregnancy CLD1
SA410338HH190425Urine Pregnancy CLD1
SA410339HH190422Urine Pregnancy CLD1
SA410340MM190425Urine Pregnancy CLD1
SA410341WW200203_1Urine Pregnancy CLD1
SA410342MM190419Urine Pregnancy CLD1
SA410343FFr170408Urine Pregnancy CLD1
SA410344WW200131_1Urine Pregnancy CLD1
SA410345FFr170405Urine Pregnancy CLD1
SA410346HH160223Urine Pregnancy CLD1
SA410347HH160225Urine Pregnancy CLD1
SA410348HH160229Urine Pregnancy CLD1
SA410349WW200214_1Urine Pregnancy CLD2
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Collection:

Collection ID:CO003909
Collection Summary:All urine samples were collected either via aspiration from the concrete floor with a clean syringe in the absence of the animal or by direct urination in a small container (trained animals). After collection, the samples were transferred to a collection vial and stored at -20°C until shipment to the Laboratory of Integrative Metabolomics (LIMET) at Ghent University (Belgium). Before analysis, urinary specific gravity (USpG) was measured using a handheld digital refractometer designed for cat (PAL-USG (Cat), range 1.000-1.080, Atago™, Fukaya, Japan) or human urine (Pal-10S (Human), range 1.000-1.060, Atago™, Fukaya, Japan). The urine samples were collected during 6 phases of the reproductive cycle: anestrus (start of observations until cross-over between progestogens and estrogens, which marks the start of the pro-estrus), early corpus luteum dormancy phase (CLD1, following ovulation, variable length), middle corpus luteum dormancy phase (CLD2, starting ± 80 days prior to birth/end of the cycle), late corpus luteum dormancy phase (CLD3, starting ± 60 days prior to birth/end of the cycle), early active luteal phase (EAL, from start of secondary, more increased progesterone peak ± 42 days prior to birth/end of the cycle until PGFM peak), and late active luteal phase (LAL, post PGFM peak until birth/end of the cycle).
Sample Type:Urine
Storage Conditions:-20℃

Treatment:

Treatment ID:TR003925
Treatment Summary:No treatment. All samples were filtered by being transferred to a 15 mL Nunc® tube (Thermo Fisher Scientific, San José, California, USA) inserted with a small-sized disposable plastic funnel with a cotton-wool filter plug.

Sample Preparation:

Sampleprep ID:SP003922
Sampleprep Summary:Samples were first centrifuged for 8 min at 1,000 g and 4°C followed by the collection of 50 µL of the supernatant in a 1.5-mL Eppendorf tube. Then, 130 µL UPW and 20 µL internal standard solution (10 ng µL-1 in UPW) were added. The diluted sample was vortexed for 30 sec after which 100 µL was transferred into a glass HPLC vial with insert.
Extract Storage:4℃

Chromatography:

Chromatography ID:CH004711
Methods Filename:Metabolomics_GP_MS2.pdf
Instrument Name:Thermo Dionex Ultimate 3000 XRS
Column Name:Waters ACQUITY UPLC HSS T3 C18 (150 x 2.1mm,1.8um)
Column Temperature:45
Flow Gradient:0-1.5 min: 2% B, 1.5-7.0 min: 2-25% B; 7.0-8.0 min: 25-60% B, 8.0-12.0 min: 60-95% B, 12.0-14.0 min: 95% B, 14.0-14.1 min: 95-2% B, 14.1-18.0 min: 2% B
Flow Rate:0.4 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% Acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN006211
Analysis Type:MS
Analysis Protocol File:Metabolomics_GP_MS2.pdf
Chromatography ID:CH004711
Num Factors:14
Num Metabolites:4
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST003782_AN006211_Results.txt
Units:AUC
  
Analysis ID:AN006212
Analysis Type:MS
Analysis Protocol File:Metabolomics_GP_MS2.pdf
Chromatography ID:CH004711
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST003782_AN006212_Results.txt
Units:AUC
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