Summary of Study ST003828
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002391. The data can be accessed directly via it's Project DOI: 10.21228/M8K82P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003828 |
| Study Title | An integrated analysis of behavioral and metabolic modulations under a chronobiotic treatment in Drosophila melanogaster |
| Study Summary | Drosophila melanogaster, with its conserved molecular clocks and accessible assays, serves as an ideal model system for studying circadian rhythms and sleep. Using an integrated approach combining high-throughput locomotor activity monitoring and untargeted metabolomics, we analyzed the behavioral and metabolic effects of a chronobiotic melatonin. The behavioral activity of flies was recorded using an infrared-based monitoring device followed by data analysis with open-source data packages, ShinyR-DAM and VANESSA. We found that 1mM and 4mM melatonin doses significantly increased Drosophila locomotor activity. Melatonin at high concentrations (4mM) exhibited protective effects by reducing mortality in Drosophila. Despite these changes, melatonin preserved the flies; endogenous bimodal activity pattern, maintaining circadian alignment. High- performance LC-MS metabolomics identified differentially abundant metabolites under drug treatment. LC-MS-based metabolomics identified 20 biologically relevant metabolites altered by melatonin, including key perturbations in arginine biosynthesis, alanine/aspartate/glutamate metabolism, and pyrimidine pathways. Notably, melatonin upregulated glutamine, a potential indicator of enhanced neurotransmitter synthesis and broadly modulated amino acid and nucleotide metabolism, suggesting dual roles in neuroprotection and energy homeostasis. This high-throughput approach uncovers melatonin- induced behavioral and metabolic perturbations, revealing how melatonin modulates locomotor activity, survival, and circadian integrity through specific alterations in metabolism. |
| Institute | Indian Institute of Technology Hyderabad |
| Department | Biotechnology |
| Laboratory | Circadian Rhythms and Disease Biology Laboratory |
| Last Name | Das |
| First Name | Sandip |
| Address | NH-9,KANDI |
| bo21resch11009@iith.ac.in | |
| Phone | 6290857805 |
| Submit Date | 2025-03-27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, d |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-05-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002391 |
| Project DOI: | doi: 10.21228/M8K82P |
| Project Title: | An integrated analysis of behavioral and metabolic modulations under a chronobiotic treatment in Drosophila melanogaster |
| Project Type: | MS based Metabolomics analysis |
| Project Summary: | Drosophila melanogaster, with its conserved molecular clocks and accessible assays, serves as an ideal model system for studying circadian rhythms and sleep. Using an integrated approach combining high-throughput locomotor activity monitoring and untargeted metabolomics, we analyzed the behavioral and metabolic effects of a chronobiotic melatonin. The behavioral activity of flies was recorded using an infrared-based monitoring device followed by data analysis with open-source data packages, ShinyR-DAM and VANESSA. We found that 1mM and 4mM melatonin doses significantly increased Drosophila locomotor activity. Melatonin at high concentrations (4mM) exhibited protective effects by reducing mortality in Drosophila. Despite these changes, melatonin preserved the flies; endogenous bimodal activity pattern, maintaining circadian alignment. High- performance LC-MS metabolomics identified differentially abundant metabolites under drug treatment. LC-MS-based metabolomics identified 20 biologically relevant metabolites altered by melatonin, including key perturbations in arginine biosynthesis, alanine/aspartate/glutamate metabolism, and pyrimidine pathways. Notably, melatonin upregulated glutamine, a potential indicator of enhanced neurotransmitter synthesis and broadly modulated amino acid and nucleotide metabolism, suggesting dual roles in neuroprotection and energy homeostasis. This high-throughput approach uncovers melatonin- induced behavioral and metabolic perturbations, revealing how melatonin modulates locomotor activity, survival, and circadian integrity through specific alterations in metabolism. |
| Institute: | Indian Institute of Technology Hyderabad |
| Department: | Biotechnology |
| Laboratory: | Circadian Rhythms and Disease Biology Laboratory |
| Last Name: | Das |
| First Name: | Sandip |
| Address: | NH-9,KANDI, sangareddy, telengana, 502285, India |
| Email: | bo21resch11009@iith.ac.in |
| Phone: | 6290857805 |
Subject:
| Subject ID: | SU003962 |
| Subject Type: | Invertebrate |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Genotype Strain: | Canton-S |
| Age Or Age Range: | 3-4 day |
| Gender: | Male |
Factors:
Subject type: Invertebrate; Subject species: Drosophila melanogaster (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA418939 | Sa_7 | Control |
| SA418940 | Sa_2 | Control |
| SA418941 | Sa_9 | Control |
| SA418942 | Sa_8 | Control |
| SA418943 | Sa_1 | Control |
| SA418944 | Sa_6 | Control |
| SA418945 | Sa_5 | Control |
| SA418946 | Sa_4 | Control |
| SA418947 | Sa_3 | Control |
| SA418948 | Sa_11 | Melatonin_Treated |
| SA418949 | Sa_12 | Melatonin_Treated |
| SA418950 | Sa_13 | Melatonin_Treated |
| SA418951 | Sa_14 | Melatonin_Treated |
| SA418952 | Sa_15 | Melatonin_Treated |
| SA418953 | Sa_16 | Melatonin_Treated |
| SA418954 | Sa_17 | Melatonin_Treated |
| SA418955 | Sa_18 | Melatonin_Treated |
| SA418956 | Sa_10 | Melatonin_Treated |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO003955 |
| Collection Summary: | Melatonin 48-h treated (2 circadian cycles) and equivalent ethanol-treated flies were considered for metabolomics. 24 flies from each vial were considered one biological sample. Three sets of these samples were created for melatonin and vehicle (biological replicates), resulting in six samples. Flies were snap-frozen using liquid nitrogen and stored frozen at −80 °C until heads were separated and used for metabolite extractionmetabolite extraction. |
| Sample Type: | Fly Head |
Treatment:
| Treatment ID: | TR003971 |
| Treatment Summary: | 3 to 4-day-old adult imago male flies were supplemented with fresh solutions of 4 mM Melatonin crystalline (the stock concentration of the drug was dissolved in 95% ethanol) in the sucrose-agar media. Equivalent volumes and concentrations of the vehicle were added to the sucrose-agar medium for vehicle treatments. |
Sample Preparation:
| Sampleprep ID: | SP003968 |
| Sampleprep Summary: | Heads were separated and used for metabolite extraction by adding a mixture of cold 120 μl methanol: chloroform (2:1). The samples were homogenized for 3 cycles on ice (3 mins on, 3 mins off). Mild sonication was applied for 15 min (30s on, 30s off; high power) using a Bioruptor Standard (Diagenode) instrument. A 200 μl of an additional solvent of water and chloroform (1:1) was added to the homogenate. After centrifugation (18,000 g for 7 minutes at 4°C), 100 μl of the aqueous layer was collected in a fresh microcentrifuge tube. The separated aqueous layer was dried down under a vacuum at 1500 rpm. The dried pellets were re-suspended in a 100 μl mixture of water and acetonitrile (1:1) |
Chromatography:
| Chromatography ID: | CH004771 |
| Instrument Name: | Agilent 6550 |
| Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm, 2.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min, 96 % B; 0-2 min 96 % B, 2-5.5 min, 96-88% B; 5.5-8.5 min, 88% B; 8.5-9 min, 88-86% B; 9-14 min, 86% B; 14-17 min, 86-82% B; 17-23 min, 82-65% B; 23-24 min, 65% B; 24-24.5 min, 65-96% B; 24.5-26 min, 96% B. |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water; 10 mM of ammonium acetate |
| Solvent B: | 85% acetonitrile/15% water; 10 mM of ammonium acetate |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN006288 |
| Analysis Type: | MS |
| Chromatography ID: | CH004771 |
| Num Factors: | 2 |
| Num Metabolites: | 20 |
| Rt Units: | Minutes |
| Units: | Abundance |
