Summary of Study ST003839

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002398. The data can be accessed directly via it's Project DOI: 10.21228/M8P249 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003839
Study TitleTaurochenodeoxycholic acid levels are associated with scar hypertrophy in the rabbit ear hypertrophic scar model.
Study SummaryNew Zealand white rabbits were used to establish the rabbit ear hypertrophic scar model. On postoperative day 28, the rabbits were divided into scar-susceptible (group H) and scar-insusceptible (group L) groups according to macroscopic scar thickness. Using fecal microbiota transplantation (FMT), we exchanged the gut microbiota of group H and group L. Untargeted metabolomics was performed before and after FMT. We found that in the rabbit ear hypertrophic scar model, scar hypertrophy was more severe in group H and was associated with fecal microbiota and their metabolites. Gut microbiota components and key genera were transferred between group H and group L by FMT, accompanied by altered levels of key metabolites such as TCDCA (bile acid taurochenodeoxycholic acid). FMT relieved scar hypertrophy in group H and vice versa. TCDCA were decreased in group H rabbit and its relative abundance was successfully reversed by heterochronic FMT.
Institute
Shenzhen Center for Chronic Disease Control
DepartmentPlastic surgery, Shenzhen Institute of Dermatology
Last NameLiang
First NameZhengyun
AddressNo. 2021, Buxin Road, Luohu District, Shenzhen 518020, China
Email18819481519@163.com
Phone18819481519
Submit Date2025-03-19
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Thermo)
Analysis Type DetailOther
Release Date2025-05-01
Release Version1
Zhengyun Liang Zhengyun Liang
https://dx.doi.org/10.21228/M8P249
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002398
Project DOI:doi: 10.21228/M8P249
Project Title:Taurochenodeoxycholic acid levels are associated with scar hypertrophy in the rabbit ear hypertrophic scar model.
Project Summary:The aim of this study was to investigate the relationship between gut microbiota, metabolites and scar hypertrophy using the rabbit ear hypertrophic scar model. On postoperative day 28, the rabbits were divided into scar-susceptible (group H) and scar-insusceptible (group L) groups according to macroscopic scar thickness. Using fecal microbiota transplantation (FMT), we exchanged the gut microbiota of group H and group L. Untargeted metabolomics was performed before and after FMT. We found that in the rabbit ear hypertrophic scar model, scar hypertrophy was more severe in group H and was associated with fecal microbiota and their metabolites. Gut microbiota components and key genera were transferred between group H and group L by FMT, accompanied by altered levels of key metabolites such as TCDCA (bile acid taurochenodeoxycholic acid). FMT relieved scar hypertrophy in group H and vice versa. TCDCA were decreased in group H rabbit and its relative abundance was successfully reversed by heterochronic FMT.
Institute:Shenzhen Center for Chronic Disease Control
Department:Plastic surgery, Shenzhen Institute of Dermatology
Last Name:Liang
First Name:Zhengyun
Address:No. 2021, Buxin Road, Luohu District, Shenzhen, Guangdong, 51802, China
Email:18819481519@163.com
Phone:18819481519

Subject:

Subject ID:SU003973
Subject Type:Mammal
Subject Species:Oryctolagus cuniculus
Taxonomy ID:9986
Genotype Strain:Wildtype
Gender:Male and female

Factors:

Subject type: Mammal; Subject species: Oryctolagus cuniculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Time point
SA419793A33_T4H-FMT T4
SA419794A48_T4H-FMT T4
SA419795A09_T4H-FMT T4
SA419796A30_T4H-FMT T4
SA419797A07_T4H-FMT T4
SA419798A38_T4H-FMT T4
SA419799A29_T1H T1
SA419800A05_T1H T1
SA419801A48_T1H T1
SA419802A30_T1H T1
SA419803A07_T1H T1
SA419804A38_T1H T1
SA419805A33_T1H T1
SA419806A46_T1H T1
SA419807A25_T1H T1
SA419808A09_T1H T1
SA419809A36_T4L-FMT T4
SA419810A31_T4L-FMT T4
SA419811A03_T4L-FMT T4
SA419812A40_T4L-FMT T4
SA419813A39_T4L-FMT T4
SA419814A34_T4L-FMT T4
SA419815A36_T1L T1
SA419816A39_T1L T1
SA419817A31_T1L T1
SA419818A03_T1L T1
SA419819A40_T1L T1
SA419820A34_T1L T1
SA419821A44_T1L T1
SA419822A42_T1L T1
SA419823A08_T1L T1
SA419824A06_T1L T1
Showing results 1 to 32 of 32

Collection:

Collection ID:CO003966
Collection Summary:Four full-thickness 1.5 cm in diameter round wounds were created on the ventral side of each rabbit ear using sterile surgical blades following iodophor sterilization. Sterile boxes, pads, tweezers and fecal containers were utilized for rabbit fecal pellets collection. Fecal pellets were collected at “grouping” (postoperative day 28, T1) and “end” (postoperative day 63, T4) time points.
Sample Type:Feces

Treatment:

Treatment ID:TR003982
Treatment Summary:Macroscopic scar thickness (mm) was measured weekly from postoperative day 21 using a vernier caliper. On postoperative day 28, rabbits were grouped by macroscopic scar thickness. Rabbits with MST values greater than 1.9 mm were assigned to the high-risk/susceptible group for hypertrophic scars (group H), otherwise, they were assigned to the low-risk/insusceptible group (group L). Using fecal microbiota transplantation (FMT), we exchanged the gut microbiota of group H and group L.

Sample Preparation:

Sampleprep ID:SP003979
Sampleprep Summary:After being grounded with liquid nitrogen, 100 mg rabbit fecal samples were added to 500 μL 80% methanol-water solution. After dilution and centrifugation, the supernatant with 53% methanol was collected for the liquid chromatography–mass spectrometry (LC-MS) analysis.

Chromatography:

Chromatography ID:CH004786
Chromatography Summary:Samples were injected onto a Hypesil Gold column (100×2.1 mm, 1.9μm) using a 12-min linear gradient at a flow rate of 0.2mL/min.Solvent A was 5 mM ammonium acetate and olvent B was Methanol.Solvent A was maintained at pH 9.0.The gradient was set as follows:2% B,0-1.5 min; 2-85% B,1.5-3 min; 85-100% B,3-10 min；100-2% B, 10-10.1min ；2% B,10.1-12 min.
Instrument Name:Thermo Vanquish
Column Name:Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um)
Column Temperature:40
Flow Gradient:2% B,0-1.5 min; 2-85% B,1.5-3 min; 85-100% B,3-10 min；100-2% B, 10-10.1min ；2% B,10.1-12 min.
Flow Rate:0.2 mL/min
Solvent A:100% water; 5 mM ammonium acetate
Solvent B:100% Methanol
Chromatography Type:HILIC

Analysis:

Analysis ID:AN006308
Analysis Type:MS
Chromatography ID:CH004786
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST003839_AN006308_Results.txt
Units:peak area
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