Summary of Study ST003866
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002422. The data can be accessed directly via it's Project DOI: 10.21228/M8K54D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003866 |
| Study Title | Impact of Exogenous Arginine Addition on the Bacterial Metabolome |
| Study Summary | The interaction between bacterial metabolic state modulation and antibiotic susceptibility has gained increasing attention as a strategy to combat antibiotic resistance. This study investigated the mechanism by which L-arginine enhanced the bactericidal efficiency of antibiotics. The metabolites in the exogenous Arg group and the control group were analyzed using LC-MS. KEGG enrichment analysis identified three significantly altered pathways: phenylalanine metabolism, degradation of aromatic compounds, and the TCA cycle. The results confirmed that arginine can change multiple metabolic pathways of bacteria, providing theoretical support for the study of arginine to change the bactericidal efficiency of antibiotics. |
| Institute | Chinese Academy of Sciences |
| Last Name | Wang |
| First Name | Chao |
| Address | NO.162 Jiefang Road, Jinan, China |
| wangchao850303@163.com | |
| Phone | 13075330325 |
| Submit Date | 2025-03-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-05-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002422 |
| Project DOI: | doi: 10.21228/M8K54D |
| Project Title: | Mechanisms of Exogenous Metabolites Alter the Bactericidal Efficiency of Antibiotics by Affecting the Metabolic State of Bacteria |
| Project Summary: | Antibiotic resistance is a serious global crisis. Different from the traditional drug resistance mechanism, metabolic regulation can change bacteria from antibiotic resistant state to sensitive state, which provides a new way for antibiotics to treat the infection of resistant bacteria. In previous studies, we found that arginine can significantly improve the bactericidal efficiency of antibiotics, but the mechanism was not clear. We plan to study the effects of arginine on the global metabolic pathway from the perspective of metabolomics, and finally determine how arginine changes the bactericidal efficiency of antibiotics. |
| Institute: | Chinese Academy of Sciences |
| Last Name: | Wang |
| First Name: | Chao |
| Address: | NO.162 Jiefang Road, Jinan, China |
| Email: | wangchao850303@163.com |
| Phone: | 13075330325 |
Subject:
| Subject ID: | SU004000 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 679895 |
Factors:
Subject type: Bacteria; Subject species: Escherichia coli (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA424684 | control 1 | Control |
| SA424685 | control 2 | Control |
| SA424686 | control 3 | Control |
| SA424687 | control 4 | Control |
| SA424688 | control 5 | Control |
| SA424689 | sample 1 | sample |
| SA424690 | sample 2 | sample |
| SA424691 | sample 3 | sample |
| SA424692 | sample 4 | sample |
| SA424693 | sample 5 | sample |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003993 |
| Collection Summary: | Random colonies of E. tarda strain ATCC 15947 were cultured overnight at 37°C in TSB medium, and cells were diluted 1:100 into fresh medium under the same incubation condition. The bacteria were harvested when OD600 was 1.0. The equivalent cells were quenched with 60% (v/v) cold of methanol at −40°C for 5 s and collected by centrifugation at 1000g at 4°C for 10 min. After removing the supernatant, the cells were stored at −80°C. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR004009 |
| Treatment Summary: | E.tarda cells diluted in M9 media with or without exogenous Arg incubated at 37°C for 5 h. Then, the metabolic reaction is terminated by cold methanol (-80°C), and bacterial cells were harvested rapidly by centrifugation at 5000g for 5 min. After washed twice by cold sterile saline, the bacterial cells were frozen by liquid nitrogen, and stored at −80°C. |
Sample Preparation:
| Sampleprep ID: | SP004006 |
| Sampleprep Summary: | After equivalent cells resuspended by 0.1 mL of precooled methanol at -20°C and incubated for 60 min, the samples were collected by centrifugation at 14,000 g for 15 min, and the supernatants were dried in a centrifugal evaporator. The dried samples were redissolved by 80% methanol and analyzed. |
Chromatography:
| Chromatography ID: | CH004819 |
| Instrument Name: | Vanquish UHPLC system |
| Column Name: | Agilent ZORBAX Eclipse Plus C18 (100 x 2.1 mm, 3.5 µm) |
| Column Temperature: | 25°C |
| Flow Gradient: | 2%B for 1min, 2−50%B for 16.5min, and 50−2%B for 2.5 min |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 95% Acetonitrile; 5% Water |
| Solvent B: | 50% Acetonitrile; 50% Water |
| Chromatography Type: | Normal phase |
Analysis:
| Analysis ID: | AN006354 |
| Analysis Type: | MS |
| Chromatography ID: | CH004819 |
| Has Mz: | 1 |
| Rt Units: | No RT data |
| Results File: | ST003866_AN006354_Results.txt |
| Units: | peak area |
