Summary of Study ST003875
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002429. The data can be accessed directly via it's Project DOI: 10.21228/M8NZ5B This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003875 |
| Study Title | Glia-derived secretory fatty acid binding protein Obp44a regulates lipid storage and efflux in the developing Drosophila brain |
| Study Summary | Glia derived secretory factors play diverse roles in supporting the development, physiology, and stress responses of the central nervous system (CNS). Through transcriptomics and imaging analyses, we have identified Obp44a as one of the most abundantly produced secretory proteins from Drosophila CNS glia. Protein structure homology modeling and Nuclear Magnetic Resonance (NMR) experiments reveal Obp44a as a fatty acid binding protein (FABP) with a high affinity towards long-chain fatty acids in both native and oxidized forms. Further analyses demonstrate that Obp44a effectively infiltrates the neuropil, traffics between neuron and glia, and is secreted into hemolymph, acting as a lipid chaperone and scavenger to regulate lipid and redox homeostasis in the developing brain. In agreement with this essential role, deficiency of Obp44a leads to anatomical and behavioral deficits in adult animals and elevated oxidized lipid levels. Collectively, our findings unveil the crucial involvement of a noncanonical lipid chaperone to shuttle fatty acids within and outside the brain, as needed to maintain a healthy brain lipid environment. These findings could inspire the design of novel approaches to restore lipid homeostasis that is dysregulated in CNS diseases. |
| Institute | Advanced Science Research Center - CUNY |
| Department | Neuroscience |
| Laboratory | He Lab |
| Last Name | He |
| First Name | Ye |
| Address | 85 St. Nicholas Terrace, Room |
| yhe1@gc.cuny.edu | |
| Phone | 12124133182 |
| Submit Date | 2025-04-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-07-07 |
| Release Version | 1 |
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Project:
| Project ID: | PR002429 |
| Project DOI: | doi: 10.21228/M8NZ5B |
| Project Title: | Glia-derived secretory fatty acid binding protein Obp44a regulates lipid storage and efflux in the developing Drosophila brain |
| Project Summary: | Glia derived secretory factors play diverse roles in supporting the development, physiology, and stress responses of the central nervous system (CNS). Through transcriptomics and imaging analyses, we have identified Obp44a as one of the most abundantly produced secretory proteins from Drosophila CNS glia. Protein structure homology modeling and Nuclear Magnetic Resonance (NMR) experiments reveal Obp44a as a fatty acid binding protein (FABP) with a high affinity towards long-chain fatty acids in both native and oxidized forms. Further analyses demonstrate that Obp44a effectively infiltrates the neuropil, traffics between neuron and glia, and is secreted into hemolymph, acting as a lipid chaperone and scavenger to regulate lipid and redox homeostasis in the developing brain. In agreement with this essential role, deficiency of Obp44a leads to anatomical and behavioral deficits in adult animals and elevated oxidized lipid levels. Collectively, our findings unveil the crucial involvement of a noncanonical lipid chaperone to shuttle fatty acids within and outside the brain, as needed to maintain a healthy brain lipid environment. These findings could inspire the design of novel approaches to restore lipid homeostasis that is dysregulated in CNS diseases. |
| Institute: | Advanced Science Research Center - CUNY |
| Department: | Neuroscience |
| Laboratory: | He Lab |
| Last Name: | He |
| First Name: | Ye |
| Address: | 85 St. Nicholas Terrace, New York, New York, 10031, USA |
| Email: | yhe1@gc.cuny.edu |
| Phone: | 2124133182 |
Subject:
| Subject ID: | SU004010 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
Factors:
Subject type: Insect; Subject species: Drosophila melanogaster (Factor headings shown in green)
| mb_sample_id | local_sample_id | genotype |
|---|---|---|
| SA426414 | KO3_001 | Obp44a_KO |
| SA426415 | KO1_002 | Obp44a_KO |
| SA426416 | KO3_003 | Obp44a_KO |
| SA426417 | KO3_002 | Obp44a_KO |
| SA426418 | KO1_001 | Obp44a_KO |
| SA426419 | KO2_003 | Obp44a_KO |
| SA426420 | KO2_002 | Obp44a_KO |
| SA426421 | KO2_001 | Obp44a_KO |
| SA426422 | KO1_003 | Obp44a_KO |
| SA426423 | WT1_002 | Wild-type |
| SA426424 | WT1_003 | Wild-type |
| SA426425 | WT2_001 | Wild-type |
| SA426426 | WT2_002 | Wild-type |
| SA426427 | WT2_003 | Wild-type |
| SA426428 | WT3_001 | Wild-type |
| SA426429 | WT3_002 | Wild-type |
| SA426430 | WT3_003 | Wild-type |
| SA426431 | WT1_001 | Wild-type |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO004003 |
| Collection Summary: | For each biological replicate, a pool of 100 brains from third instar larvae of either wild type (Canton-S) or Obp44a-/- was dissected and combined in PBS buffer. |
| Sample Type: | Brain |
Treatment:
| Treatment ID: | TR004019 |
| Treatment Summary: | For each biological replicate, a pool of 100 brains from third instar larvae of either genotype was dissected and combined in PBS buffer. |
Sample Preparation:
| Sampleprep ID: | SP004016 |
| Sampleprep Summary: | To extract metabolites, the brain samples were homogenized in 200 μl of cold Methanol/Water solution (80/20, v/v) and subjected to gentle sonication using a Bioruptor instrument (30 s on, 30 s off, 10 cycles) at 4°C. Subsequently, the lysates were centrifuged at 10,000 x g for 10 minutes at 4°C, and the resulting supernatants were collected for LC-MS/MS analysis. |
Combined analysis:
| Analysis ID | AN006367 |
|---|---|
| Chromatography ID | CH004830 |
| MS ID | MS006068 |
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker Daltonics maXis-II |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH004830 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| Column Temperature: | 30°C |
| Flow Gradient: | 0.15mL/min; 0-5.0 min; 2.0% B, 5.0-28.0 min; 2.0-60.0% B, 28.0-38.0 min; 60.0% B, 38.0-39.0 min; 60.0-2.0% B, 39.0-48.0 min, 2.0% B. |
| Flow Rate: | 0.15 mL/min |
| Solvent A: | 97% Acetonitrile/3% Water; 7mM Ammonium acetate |
| Solvent B: | 97% Water/3% Acetonitrile; 7mM Ammonium acetate |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS006068 |
| Analysis ID: | AN006367 |
| Instrument Name: | Bruker Daltonics maXis-II |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS spectra were obtained using a Bruker maXis-II-ETD UHR-ESI-QTOF, witb Ultra High Resolution QTOF (UHR) technology in addition to Electron-Transfer Dissociation (ETD). Compound identification and descriptive statistical analysis of the LC-MS/MS data were performed through Metaboscape and XCMSPlus software. Bruker MetaboBase Personal 3.0, MoNA, MSDIAL, METLIN, and HMDB metabolomic libraries were used in compound identification. Ultimately, both accurate mass-measurements (with less than 5 pmm accuracy) and fragmentation spectra (or simply MS/MS spectra) were used for confident identification of metabolites and lipids. |
| Ion Mode: | POSITIVE |
