Summary of Study ST003894

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002438. The data can be accessed directly via it's Project DOI: 10.21228/M8H53D This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003894
Study TitlePostprandial Plasma Lipidomic Changes in 24 Metabolically Healthy Individuals Following Ingestion of Four Different Isocaloric Macronutrients
Study SummaryThis study enrolled 24 metabolically healthy adults who were administered isocaloric loads of glucose, protein, butter, or olive oil in a randomized fashion. Plasma samples were collected at multiple time points post-ingestion, and untargeted lipidomic profiling was performed to assess dynamic changes in lipid species. The results revealed distinct lipidomic responses to different macronutrients, providing important insights into how specific dietary components regulate lipid metabolism.We found that under glucose load stimulation, NAE 18:1, CAR 16:2, and CAR 14:0 changed significantly. Under protein load stimulation, Cer 42:2;2O|Cer 18:2;2O/24:0, PC 32:3, and PC 34:1 showed significant changes. Under olive oil load stimulation, LPC 20:3/0:0, TG 48:2;1O|TG 14:0_16:0_18:2;1O, and 14:0_16:0_18:2;1O changed significantly. Under butter load stimulation, TG 55:5|TG 17:0_18:1_20:4, TG 58:7|TG 18:1_18:2_22:4, and TG 57:8|TG 18:1_18:2_21:5 showed significant changes.
Institute
Nanjing Medical University
Last NameWang
First NameJiachen
AddressNo. 300, Guangzhou Road, Nanjing
Emailjcwang@stu.njmu.edu.cn
Phone02565306466
Submit Date2025-04-27
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Thermo)
Analysis Type DetailOther
Release Date2025-07-30
Release Version1
Jiachen Wang Jiachen Wang
https://dx.doi.org/10.21228/M8H53D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002438
Project DOI:doi: 10.21228/M8H53D
Project Title:Postprandial Plasma Multi-Omics Changes in Response to a Standard Mixed Meal or Individual Macronutrients in Humans
Project Summary:Postprandial metabolism is a complex and dynamic physiological process involving numerous metabolic pathways and biomolecular interactions. It plays a critical role in the development of chronic diseases such as obesity, diabetes, and cardiovascular disease. This study employed an interventional design in human participants to systematically compare the dynamic postprandial changes in plasma multi-omics profiles following ingestion of a standard mixed meal versus four individual macronutrients (glucose, protein, butter, and olive oil). The aim is to elucidate the specific effects of different nutrients on human postprandial metabolic networks and their potential implications for health, thereby providing foundational data to support personalized nutrition strategies and disease prevention.
Institute:Nanjing Medical University
Last Name:Wang
First Name:Jiachen
Address:No. 300, Guangzhou Road, Nanjing
Email:jcwang@stu.njmu.edu.cn
Phone:+86 02565306466

Subject:

Subject ID:SU004029
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time
SA4276963P04A0
SA4276972P22A0
SA4276982P23A0
SA4276992P25A0
SA4277001P02A0
SA4277013P02A0
SA4277023P03A0
SA4277033P05A0
SA4277042P20A0
SA4277053P06A0
SA4277063P07A0
SA4277073P08A0
SA4277083P09A0
SA4277093P10A0
SA4277103P11A0
SA4277112P21A0
SA4277122P19A0
SA4277133P13A0
SA4277142P09A0
SA4277152P03A0
SA4277162P04A0
SA4277172P05A0
SA4277182P06A0
SA4277192P07A0
SA4277202P08A0
SA4277212P10A0
SA4277222P18A0
SA4277232P11A0
SA4277242P12A0
SA4277252P13A0
SA4277262P14A0
SA4277272P15A0
SA4277282P16A0
SA4277292P17A0
SA4277303P12A0
SA4277313P14A0
SA4277322P01A0
SA4277334P17A0
SA4277344P11A0
SA4277354P12A0
SA4277364P13A0
SA4277374P14A0
SA4277384P15A0
SA4277394P16A0
SA4277404P18A0
SA4277414P09A0
SA4277424P19A0
SA4277434P20A0
SA4277444P21A0
SA4277454P22A0
SA4277464P23A0
SA4277474P25A0
SA4277484P10A0
SA4277494P08A0
SA4277503P15A0
SA4277513P22A0
SA4277523P16A0
SA4277533P17A0
SA4277543P18A0
SA4277553P19A0
SA4277563P20A0
SA4277573P21A0
SA4277583P23A0
SA4277594P07A0
SA4277603P25A0
SA4277614P01A0
SA4277624P02A0
SA4277634P03A0
SA4277644P04A0
SA4277654P05A0
SA4277664P06A0
SA4277672P02A0
SA4277681P01A0
SA4277693P01A0
SA4277701P14A0
SA4277711P05A0
SA4277721P07A0
SA4277731P08A0
SA4277741P09A0
SA4277751P10A0
SA4277761P11A0
SA4277771P12A0
SA4277781P13A0
SA4277791P15A0
SA4277801P03A0
SA4277811P16A0
SA4277821P17A0
SA4277831P18A0
SA4277841P19A0
SA4277851P20A0
SA4277861P21A0
SA4277871P22A0
SA4277881P23A0
SA4277891P25A0
SA4277901P04A0
SA4277911P06A0
SA4277922P25D120
SA4277933P03D120
SA4277942P07D120
SA4277952P06D120
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Collection:

Collection ID:CO004022
Collection Summary:Blood samples were collected at fasting baseline (0 min) and at 30, 60, 120, and 180 min post-consumption (a total of 480 samples) and stored at -80 C until analysis.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR004038
Treatment Summary:A cohort of healthy volunteers was recruited for the Single Macronutrient Tolerance Test (SMNTT). The inclusion criteria were: age ≥ 18 years old, no history of abnormal glucose levels (fasting plasma glucose < 6 mmol/L, 2-hour postprandial plasma glucose <7.8 mmol/L); normal body mass index (BMI, 18–24 kg/m²); no family history of diabetes; no history of malignancy, severe organ diseases, or significant acute or chronic infections; and no recent use of glucocorticoids, immunosuppressants, or medications known to significantly affect metabolism. A total of 12 healthy men and 12 healthy women, aged 18-40 years, completed the SMNTT. Participants underwent four successive SMNTT (glucose, whey protein, butter, and olive oil), each separated by a one-week interval. We used 75g of anhydrous glucose powder, 78.5g of protein powder, 40.1g butter or 33.9g olive oil for each load. Each test provided 300 kcal of energy. After a 12-hour overnight fast, participants consumed one of the four nutrients: glucose or whey protein dissolved in 250 mL of water, olive oil consumed directly, or butter accompanied by three lettuce leaves.

Sample Preparation:

Sampleprep ID:SP004035
Sampleprep Summary:Ice-cold methanol and methyl tert-butyl ether (MTBE, Optima brand, Fisher Scientific) was sequentially added into plasma sample, mixture was incubated at room temperature for 30 min. Phase separation was induced by adding and incubation with MS-grade water at room temperature for 10 min, and centrifuged at 1,000 g for 10 min. The upper organic phase was collected and dried in a vacuum centrifuge. All non-polar extracts were resuspended in 75% isopropanol (IPA) in water. The temperature of the autosampler during LC-MS analyses were stored at 4°C. Samples were vortexed, centrifuged (21,000g, 20 min, 4°C) and the supernatant was aliquoted into a HPLC vial for the LC-MS analysis. Metabolic extracts were separated on BEH C18 column (Waters, SKU: 186002352, 100 mm × 2.1 mm, 1.7 μm) using a 20-min gradient with organic phase increased from 30% to 100% at 40℃(solvent A: 0.1% formic acid, 2 mM ammonium formate in 60/40 acetonitrile/water; solvent B: 2 mM ammonium formate in 90/10 IPA/acetonitrile). LC-MS analyses were performed as described above in the metabolomics section.

Chromatography:

Chromatography ID:CH004849
Chromatography Summary:DDA lipidomics analysis was performed using a Thermo Vanquish (Thermo Fisher Scientific) with Q Exative Plus orbitrap high-resolution mass spectrometry (Thermo Fisher Scientific, USA), data acquisition software XCalibur 4.3 (Thermo Fisher Scientific). The main parameters are as follows. C18 detection mode: mobile phase was water: ACN(40:60)/ IPA: ACN(90:10), solvent was added with 0.1% FA and 2 mM ammonium formate, AcquityTM BEH C18 column (Waters Co., USA, 1.7 μm, 2.1×100 mm), separation gradient: the organic phase was increased from 30% to 100% in 20 min, and the remaining 5 min was used to flush and equilibrate the column; flow rate: 0.3 mL/min, injection volume: 5 μL, column temperature: 40 °C. The detection was carried out in positive and negative ion modes, respectively.
Instrument Name:Waters Acquity
Column Name:Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:the organic phase was increased from 30% to 100% in 20 min, and the remaining 5 min was used to flush and equilibrate the column
Flow Rate:0.3 mL/min
Solvent A:40% water/60% acetonitrile
Solvent B:90% isopropanol/10% acetonitrile
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN006397
Analysis Type:MS
Chromatography ID:CH004849
Num Factors:5
Num Metabolites:436
Units:Normalized counts
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