Summary of Study ST003900
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002440. The data can be accessed directly via it's Project DOI: 10.21228/M87N91 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003900 |
| Study Title | Lipidome profiling of fly head tissues from wild-type and tty mutant flies (cohort 2) |
| Study Summary | Cohort 2 contained 6 independent lipid samples. Each lipid sample was extracted from a group of 40 fly heads of identical genotype. In total, 3 samples were isolated from each of the 2 genotypes: wild-type (w-1118) and tty mutant (tty-MI12269). Positive-mode LC-MS/MS was performed on the lipid extracts to compare the contents between the two genotypes. The results showed increased levels of triglycerides and diacylglycerides in tty mutants. |
| Institute | Rutgers University |
| Last Name | Wong |
| First Name | Ching-On |
| Address | 195 University Avenue, Boyden Hall Room 206, Newark, NJ 07102 |
| chingon.wong@rutgers.edu | |
| Phone | 19733530832 |
| Submit Date | 2025-05-02 |
| Num Groups | 2 |
| Total Subjects | 14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-05-28 |
| Release Version | 1 |
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Project:
| Project ID: | PR002440 |
| Project DOI: | doi: 10.21228/M87N91 |
| Project Title: | Loss of tty alters lipid profile in Drosophila brains |
| Project Summary: | Tweety homolog 1 (TTYH1) is an understudied protein designated by the Illuminating the Druggable Genome Program of the NIH. While previous studies implicated its functions in neural stem cell signaling and glioma invasion, little is known about the molecular function of TTYH1. Recent cryo-electron microscopy examination of TTYH1 revealed its potential role in transporting membrane lipids, leading to our hypothesis that TTYH1 regulates intracellular lipid homeostasis. Orthologs of TTYH1 have been identified across animal species including worm and fruit fly. Similar to human TTYH1, fly TTYH1 ortholog, named tweety (tty), is also predominantly expressed in the brain. To examine whether TTYH1 exerts its cellular functions via regulating lipid homeostasis, we profiled the lipidome in fruit fly (Drosophila melanogaster) head tissues isolated from wild-type (w-1118 strain) and tty mutant (tty-MI12269 strain) flies. |
| Institute: | Rutgers University Newark |
| Laboratory: | Ching-On Wong |
| Last Name: | Wong |
| First Name: | Ching-On |
| Address: | 195 University Avenue, Boyden Hall Room 206, Newark, NJ 07102 |
| Email: | chingon.wong@rutgers.edu |
| Phone: | 9733530832 |
| Contributors: | Ho Hang Leung, Ching-On Wong, Yujue Wang, Xiaoyang Su |
Subject:
| Subject ID: | SU004035 |
| Subject Type: | Invertebrate |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Age Or Age Range: | 1 week old |
| Gender: | Male and female |
Factors:
Subject type: Invertebrate; Subject species: Drosophila melanogaster (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA429586 | Fly_tty_5 | tty-MI12269 |
| SA429587 | Fly_tty_6 | tty-MI12269 |
| SA429588 | Fly_tty_7 | tty-MI12269 |
| SA429589 | Fly_w1118_5 | w-1118 |
| SA429590 | Fly_w1118_6 | w-1118 |
| SA429591 | Fly_w1118_7 | w-1118 |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO004028 |
| Collection Summary: | For each biological replicate, a total of 40 fly heads collected from snap freezing was homogenized in MTBE (Methyl tert-butyl ether) at 4°C. |
| Sample Type: | Insect tissue |
Treatment:
| Treatment ID: | TR004044 |
| Treatment Summary: | No treatment was done on the flies. |
Sample Preparation:
| Sampleprep ID: | SP004041 |
| Sampleprep Summary: | After the addition of methanol spiked with SPLASH LIPIDOMIX (330707; Avanti) as the internal standard, the homogenized mixture in MTBE was mixed briefly and incubated for 5 minutes. At endpoint, the upper organic phase was transferred into a collection tube and dried. |
Combined analysis:
| Analysis ID | AN006403 |
|---|---|
| Chromatography ID | CH004855 |
| MS ID | MS006104 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3 mm, 2.7 μm) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | ion count |
Chromatography:
| Chromatography ID: | CH004855 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3 mm, 2.7 μm) |
| Column Temperature: | 55°C |
| Flow Gradient: | 0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B. |
| Flow Rate: | 200 μL/min |
| Solvent A: | 90% Water/10% Methanol; 34.2 mM Acetic acid; 1 mM Ammonium acetate (pH 9.4) |
| Solvent B: | 75% Isopropyl alcohol/25% methanol; 34.2 mM Acetic acid; 1 mM Ammonium acetate (pH 9.4) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS006104 |
| Analysis ID: | AN006403 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Full-scan mass spectrometry analysis was performed using a Thermo Q Exactive PLUS equipped with a HESI source. The spray voltage was -2.7 kV in negative mode and 3.5 kV in positive mode. Sheath gas, auxiliary gas, and sweep gas flow rates were set at 40, 10, and 2 (arbitrary units), respectively. The capillary temperature was 300°C, and the auxiliary gas heater was set to 360°C. The S-lens RF level was 45, with an m/z scan range of 100 to 1200 m/z for both positive and negative ionization modes. Metabolite data was processed using the MAVEN software package with a mass accuracy window of 5 ppm. |
| Ion Mode: | POSITIVE |
