Summary of Study ST003939
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002469. The data can be accessed directly via it's Project DOI: 10.21228/M8H26H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003939 |
| Study Title | Plasma amino acids of treated metabolic syndrome patients with and without mild cognitive impairment |
| Study Summary | In this study, we validated mass spectrometry-based quantitation methods and quantified amino acids in plasma of ninety-five treated MetS patients with and without MCI assessed by Montreal cognitive assessment. |
| Institute | Mahidol University |
| Department | Faculty of Medicine Siriraj Hospital |
| Laboratory | SiCORE-MSB |
| Last Name | Jariyasopit |
| First Name | Narumol |
| Address | 2 Prannok, Bangkok, Non-US, 10700, Thailand |
| narumoljariyasopit@gmail.com | |
| Phone | +6624195500 |
| Submit Date | 2025-05-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC-MS |
| Release Date | 2025-06-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002469 |
| Project DOI: | doi: 10.21228/M8H26H |
| Project Title: | Higher Plasma Kynurenine to Tryptophan Correlates with Increased Incidence of Mild Cognitive Impairment in Treated Metabolic Syndrome Patients |
| Project Type: | MS quantitative analysis |
| Project Summary: | An increase in cognitive impairment has been observed in metabolic syndrome (MetS) patients. Although alterations in metabolomic profiles have been identified as potential plasma/serum biomarkers of mild cognitive impairment (MCI) and MetS, findings remain inconsistent— likely due to the heterogeneity among MetS patients and the lack of subsequent validation using targeted analysis after initial untargeted analysis. In this study, we validated mass spectrometry-based quantitation methods and quantified amino acids, fatty acids, and tryptophan metabolites in the kynurenine pathway in plasma of ninety-five treated MetS patients with and without MCI assessed by Montreal cognitive assessment. We found that MCI was positively associated with kynurenine to tryptophan ratio (KTR) after the adjustment for age, gender, and BMI, as well as were negatively associated with C20:3 [all-Z-8,11,14] and lysine. One-unit increase in KTR resulted in increased probability of developing MCI by 371%. In contrast, one-unit increases in C20:3 and lysine were associated with decreased odds of developing MCI by 81% and 78%, respectively. Our finding underscores the prominent neuroinflammation, beyond normal aging, in MetS patients, even under ongoing clinical treatment. It also points to the potential of KTR as a risk marker for MCI, offering a valuable complement to the existing cognitive assessments that may be influenced by educational background. In addition, the validated metabolite data is an useful resource for future research. It can facilitate comparisons across different studies, contribute to large-scale analyses, and be used in machine learning models for discovering and validating new biomarkers. |
| Institute: | Mahidol University |
| Department: | Faculty of Medicine Siriraj Hospital |
| Laboratory: | SiCORE-MSB |
| Last Name: | Jariyasopit |
| First Name: | Narumol |
| Address: | 2 Prannok, Bangkok, Non-US, 10700, Thailand |
| Email: | narumoljariyasopit@gmail.com |
| Phone: | 662-4195507 |
Subject:
| Subject ID: | SU004075 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
| Human Ethnicity: | Thai |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Factor |
|---|---|---|---|
| SA448822 | NC-087 | plasma | MetS |
| SA448823 | NC-098 | plasma | MetS |
| SA448824 | NC-040 | plasma | MetS |
| SA448825 | NC-042 | plasma | MetS |
| SA448826 | NC-046 | plasma | MetS |
| SA448827 | NC-047 | plasma | MetS |
| SA448828 | NC-089 | plasma | MetS |
| SA448829 | NC-055 | plasma | MetS |
| SA448830 | NC-034 | plasma | MetS |
| SA448831 | NC-056 | plasma | MetS |
| SA448832 | NC-057 | plasma | MetS |
| SA448833 | NC-079 | plasma | MetS |
| SA448834 | NC-078 | plasma | MetS |
| SA448835 | NC-061 | plasma | MetS |
| SA448836 | NC-067 | plasma | MetS |
| SA448837 | NC-100 | plasma | MetS |
| SA448838 | NC-041 | plasma | MetS |
| SA448839 | NC-113 | plasma | MetS |
| SA448840 | NC-110 | plasma | MetS |
| SA448841 | NC-029 | plasma | MetS |
| SA448842 | NC-020 | plasma | MetS |
| SA448843 | NC-012 | plasma | MetS |
| SA448844 | NC-011 | plasma | MetS |
| SA448845 | NC-010 | plasma | MetS |
| SA448846 | NC-115 | plasma | MetS |
| SA448847 | NC-080 | plasma | MetS-MCI |
| SA448848 | NC-114 | plasma | MetS-MCI |
| SA448849 | NC-116 | plasma | MetS-MCI |
| SA448850 | NC-084 | plasma | MetS-MCI |
| SA448851 | NC-077 | plasma | MetS-MCI |
| SA448852 | NC-076 | plasma | MetS-MCI |
| SA448853 | NC-075 | plasma | MetS-MCI |
| SA448854 | NC-074 | plasma | MetS-MCI |
| SA448855 | NC-073 | plasma | MetS-MCI |
| SA448856 | NC-126 | plasma | MetS-MCI |
| SA448857 | NC-081 | plasma | MetS-MCI |
| SA448858 | NC-104 | plasma | MetS-MCI |
| SA448859 | NC-085 | plasma | MetS-MCI |
| SA448860 | NC-071 | plasma | MetS-MCI |
| SA448861 | NC-102 | plasma | MetS-MCI |
| SA448862 | NC-101 | plasma | MetS-MCI |
| SA448863 | NC-106 | plasma | MetS-MCI |
| SA448864 | NC-099 | plasma | MetS-MCI |
| SA448865 | NC-107 | plasma | MetS-MCI |
| SA448866 | NC-108 | plasma | MetS-MCI |
| SA448867 | NC-095 | plasma | MetS-MCI |
| SA448868 | NC-086 | plasma | MetS-MCI |
| SA448869 | NC-094 | plasma | MetS-MCI |
| SA448870 | NC-093 | plasma | MetS-MCI |
| SA448871 | NC-111 | plasma | MetS-MCI |
| SA448872 | NC-112 | plasma | MetS-MCI |
| SA448873 | NC-092 | plasma | MetS-MCI |
| SA448874 | NC-103 | plasma | MetS-MCI |
| SA448875 | NC-072 | plasma | MetS-MCI |
| SA448876 | NC-001 | plasma | MetS-MCI |
| SA448877 | NC-070 | plasma | MetS-MCI |
| SA448878 | NC-024 | plasma | MetS-MCI |
| SA448879 | NC-037 | plasma | MetS-MCI |
| SA448880 | NC-036 | plasma | MetS-MCI |
| SA448881 | NC-035 | plasma | MetS-MCI |
| SA448882 | NC-033 | plasma | MetS-MCI |
| SA448883 | NC-031 | plasma | MetS-MCI |
| SA448884 | NC-026 | plasma | MetS-MCI |
| SA448885 | NC-025 | plasma | MetS-MCI |
| SA448886 | NC-023 | plasma | MetS-MCI |
| SA448887 | NC-039 | plasma | MetS-MCI |
| SA448888 | NC-022 | plasma | MetS-MCI |
| SA448889 | NC-021 | plasma | MetS-MCI |
| SA448890 | NC-013 | plasma | MetS-MCI |
| SA448891 | NC-009 | plasma | MetS-MCI |
| SA448892 | NC-008 | plasma | MetS-MCI |
| SA448893 | NC-005 | plasma | MetS-MCI |
| SA448894 | NC-003 | plasma | MetS-MCI |
| SA448895 | NC-038 | plasma | MetS-MCI |
| SA448896 | NC-043 | plasma | MetS-MCI |
| SA448897 | NC-069 | plasma | MetS-MCI |
| SA448898 | NC-059 | plasma | MetS-MCI |
| SA448899 | NC-068 | plasma | MetS-MCI |
| SA448900 | NC-066 | plasma | MetS-MCI |
| SA448901 | NC-065 | plasma | MetS-MCI |
| SA448902 | NC-064 | plasma | MetS-MCI |
| SA448903 | NC-063 | plasma | MetS-MCI |
| SA448904 | NC-002 | plasma | MetS-MCI |
| SA448905 | NC-060 | plasma | MetS-MCI |
| SA448906 | NC-058 | plasma | MetS-MCI |
| SA448907 | NC-044 | plasma | MetS-MCI |
| SA448908 | NC-054 | plasma | MetS-MCI |
| SA448909 | NC-053 | plasma | MetS-MCI |
| SA448910 | NC-052 | plasma | MetS-MCI |
| SA448911 | NC-051 | plasma | MetS-MCI |
| SA448912 | NC-050 | plasma | MetS-MCI |
| SA448913 | NC-049 | plasma | MetS-MCI |
| SA448914 | NC-048 | plasma | MetS-MCI |
| SA448915 | NC-045 | plasma | MetS-MCI |
| SA448916 | NC-062 | plasma | MetS-MCI |
| Showing results 1 to 95 of 95 |
Collection:
| Collection ID: | CO004068 |
| Collection Summary: | Blood was collected in EDTA-coated tubes and centrifuged at 3,000 rpm to obtain plasma. The plasma was then transferred into cryovials and stored at –85°C until use. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR004084 |
| Treatment Summary: | All patients were divided into two groups: 1) MetS patients with MCI and 2) MetS patients with normal cognitive function. MCI was determined when the MoCA score was less than 23. |
Sample Preparation:
| Sampleprep ID: | SP004081 |
| Sampleprep Summary: | Sample extraction of amino acids from plasma was modified from a published method. In brief, 30 µL of plasma was extracted with 1 mL of pre-cooled mixture of acetonitrile/isopropanol/water (3:3:2, v/v/v), containing isotopically labeled internal standards that include alanine-d3 and phenylalanine-d5 with final concentrations of ~ 5 ng/µL. The extracts were shaken at 2,000 rpm for 2 min using a vortex mixture (Scientific Industries) before being kept at -20ºC for 1 h. The extracts were then centrifuged for 10 min at 4ºC and 19,600 ×g. A 450-µL aliquot of supernatant was evaporated to dryness using a vacuum concentrator (Labconco, MO, USA) and kept at -20ºC until analysis. Prior to derivatization, the dried aliquot was resuspended in 450 µL acetonitrile/water (50:50, v/v) followed by centrifugation at 14,000 ×g and room temperature for 2 min. The supernatant was transferred to a new Eppendorf tube and dried at 50ºC under vacuum. The dried sample was reconstituted in 450 µL acetonitrile/water (50:50, v/v) before centrifugation at 14,000 ×g for 2 min at room temperature. The supernatant was evaporated to dryness. The dried samples were derivatized with 50 µL N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-butyldimethylchlorosilane (MTBSTFA + 1% TBDMSCl) and 50 µL acetonitrile, followed by incubating at 100ºC for 4 h. |
| Sampleprep Protocol Filename: | AA.txt |
Chromatography:
| Chromatography ID: | CH004914 |
| Chromatography Summary: | The quantification of amino acids was carried out using a GC-TOFMS (Pegasus HRT+4D, Leco Corp., St.Joseph, MI). The separation was achieved on a nonpolar Rxi-5sil MS column (5% diphenyl-methyl polysiloxane and 95% dimethylpolysiloxane, 30 m × 0.25 mm I.D., 0.25 μM film thickness, Restek). The injection volume was 1 µL, with the injector temperature of 250 ºC. in The samples were analyzed in splitless mode. Helium was used as a carrier gas at a constant flow rate of 1 mL min-1. The GC oven temperature program started at 50 ºC (2 min hold) and ramped to 320ºC at 20 ºC min-1 (3 min hold). Transfer line and electron impact (EI) ion source temperatures were kept at 320ºC and 250ºC, respectively. |
| Instrument Name: | Agilent 7890B |
| Column Name: | Restek Rxi-5Sil MS GC Capillary Column (30 m, 0.25 mm, 0.25 µm) |
| Column Temperature: | programmed |
| Flow Gradient: | n/a |
| Flow Rate: | 1 mL/min |
| Solvent A: | n/a |
| Solvent B: | n/a |
| Chromatography Type: | GC |
Analysis:
| Analysis ID: | AN006468 |
| Analysis Type: | MS |
| Chromatography ID: | CH004914 |
| Num Factors: | 2 |
| Num Metabolites: | 13 |
| Units: | µmol/dL plasma |
