Summary of Study ST003947

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002469. The data can be accessed directly via it's Project DOI: 10.21228/M8H26H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003947
Study Title	Total fatty acids in plasma of treated metabolic syndrome patients with and without mild cognitive impairment
Study Summary	n this study, we validated mass spectrometry-based quantitation methods and quantified total fatty acids in plasma of ninety-five treated MetS patients with and without MCI assessed by Montreal cognitive assessment.
Institute	Mahidol University
Department	Faculty of Medicine Siriraj Hospital
Laboratory	SiCORE-MSB
Last Name	Jariyasopit
First Name	Narumol
Address	2 Prannok, Bangkok, Non-US, 10700, Thailand
Email	narumoljariyasopit@gmail.com
Phone	+6624195500
Submit Date	2025-05-28
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	GC-MS
Release Date	2025-06-25
Release Version	1

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Project:

Project ID:	PR002469
Project DOI:	doi: 10.21228/M8H26H
Project Title:	Higher Plasma Kynurenine to Tryptophan Correlates with Increased Incidence of Mild Cognitive Impairment in Treated Metabolic Syndrome Patients
Project Type:	MS quantitative analysis
Project Summary:	An increase in cognitive impairment has been observed in metabolic syndrome (MetS) patients. Although alterations in metabolomic profiles have been identified as potential plasma/serum biomarkers of mild cognitive impairment (MCI) and MetS, findings remain inconsistent— likely due to the heterogeneity among MetS patients and the lack of subsequent validation using targeted analysis after initial untargeted analysis. In this study, we validated mass spectrometry-based quantitation methods and quantified amino acids, fatty acids, and tryptophan metabolites in the kynurenine pathway in plasma of ninety-five treated MetS patients with and without MCI assessed by Montreal cognitive assessment. We found that MCI was positively associated with kynurenine to tryptophan ratio (KTR) after the adjustment for age, gender, and BMI, as well as were negatively associated with C20:3 [all-Z-8,11,14] and lysine. One-unit increase in KTR resulted in increased probability of developing MCI by 371%. In contrast, one-unit increases in C20:3 and lysine were associated with decreased odds of developing MCI by 81% and 78%, respectively. Our finding underscores the prominent neuroinflammation, beyond normal aging, in MetS patients, even under ongoing clinical treatment. It also points to the potential of KTR as a risk marker for MCI, offering a valuable complement to the existing cognitive assessments that may be influenced by educational background. In addition, the validated metabolite data is an useful resource for future research. It can facilitate comparisons across different studies, contribute to large-scale analyses, and be used in machine learning models for discovering and validating new biomarkers.
Institute:	Mahidol University
Department:	Faculty of Medicine Siriraj Hospital
Laboratory:	SiCORE-MSB
Last Name:	Jariyasopit
First Name:	Narumol
Address:	2 Prannok, Bangkok, Non-US, 10700, Thailand
Email:	narumoljariyasopit@gmail.com
Phone:	662-4195507

Subject:

Subject ID:	SU004084
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female
Human Ethnicity:	Thai

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Factor
SA451023	NC-087	plasma	MetS
SA451024	NC-098	plasma	MetS
SA451025	NC-040	plasma	MetS
SA451026	NC-042	plasma	MetS
SA451027	NC-046	plasma	MetS
SA451028	NC-047	plasma	MetS
SA451029	NC-089	plasma	MetS
SA451030	NC-055	plasma	MetS
SA451031	NC-034	plasma	MetS
SA451032	NC-056	plasma	MetS
SA451033	NC-057	plasma	MetS
SA451034	NC-079	plasma	MetS
SA451035	NC-078	plasma	MetS
SA451036	NC-061	plasma	MetS
SA451037	NC-067	plasma	MetS
SA451038	NC-100	plasma	MetS
SA451039	NC-041	plasma	MetS
SA451040	NC-113	plasma	MetS
SA451041	NC-110	plasma	MetS
SA451042	NC-029	plasma	MetS
SA451043	NC-020	plasma	MetS
SA451044	NC-012	plasma	MetS
SA451045	NC-011	plasma	MetS
SA451046	NC-010	plasma	MetS
SA451047	NC-115	plasma	MetS
SA451048	NC-080	plasma	MetS-MCI
SA451049	NC-114	plasma	MetS-MCI
SA451050	NC-116	plasma	MetS-MCI
SA451051	NC-084	plasma	MetS-MCI
SA451052	NC-077	plasma	MetS-MCI
SA451053	NC-076	plasma	MetS-MCI
SA451054	NC-075	plasma	MetS-MCI
SA451055	NC-074	plasma	MetS-MCI
SA451056	NC-073	plasma	MetS-MCI
SA451057	NC-126	plasma	MetS-MCI
SA451058	NC-081	plasma	MetS-MCI
SA451059	NC-104	plasma	MetS-MCI
SA451060	NC-085	plasma	MetS-MCI
SA451061	NC-071	plasma	MetS-MCI
SA451062	NC-102	plasma	MetS-MCI
SA451063	NC-101	plasma	MetS-MCI
SA451064	NC-106	plasma	MetS-MCI
SA451065	NC-099	plasma	MetS-MCI
SA451066	NC-107	plasma	MetS-MCI
SA451067	NC-108	plasma	MetS-MCI
SA451068	NC-095	plasma	MetS-MCI
SA451069	NC-086	plasma	MetS-MCI
SA451070	NC-094	plasma	MetS-MCI
SA451071	NC-093	plasma	MetS-MCI
SA451072	NC-111	plasma	MetS-MCI
SA451073	NC-112	plasma	MetS-MCI
SA451074	NC-092	plasma	MetS-MCI
SA451075	NC-103	plasma	MetS-MCI
SA451076	NC-072	plasma	MetS-MCI
SA451077	NC-001	plasma	MetS-MCI
SA451078	NC-070	plasma	MetS-MCI
SA451079	NC-024	plasma	MetS-MCI
SA451080	NC-037	plasma	MetS-MCI
SA451081	NC-036	plasma	MetS-MCI
SA451082	NC-035	plasma	MetS-MCI
SA451083	NC-033	plasma	MetS-MCI
SA451084	NC-031	plasma	MetS-MCI
SA451085	NC-026	plasma	MetS-MCI
SA451086	NC-025	plasma	MetS-MCI
SA451087	NC-023	plasma	MetS-MCI
SA451088	NC-039	plasma	MetS-MCI
SA451089	NC-022	plasma	MetS-MCI
SA451090	NC-021	plasma	MetS-MCI
SA451091	NC-013	plasma	MetS-MCI
SA451092	NC-009	plasma	MetS-MCI
SA451093	NC-008	plasma	MetS-MCI
SA451094	NC-005	plasma	MetS-MCI
SA451095	NC-003	plasma	MetS-MCI
SA451096	NC-038	plasma	MetS-MCI
SA451097	NC-043	plasma	MetS-MCI
SA451098	NC-069	plasma	MetS-MCI
SA451099	NC-059	plasma	MetS-MCI
SA451100	NC-068	plasma	MetS-MCI
SA451101	NC-066	plasma	MetS-MCI
SA451102	NC-065	plasma	MetS-MCI
SA451103	NC-064	plasma	MetS-MCI
SA451104	NC-063	plasma	MetS-MCI
SA451105	NC-002	plasma	MetS-MCI
SA451106	NC-060	plasma	MetS-MCI
SA451107	NC-058	plasma	MetS-MCI
SA451108	NC-044	plasma	MetS-MCI
SA451109	NC-054	plasma	MetS-MCI
SA451110	NC-053	plasma	MetS-MCI
SA451111	NC-052	plasma	MetS-MCI
SA451112	NC-051	plasma	MetS-MCI
SA451113	NC-050	plasma	MetS-MCI
SA451114	NC-049	plasma	MetS-MCI
SA451115	NC-048	plasma	MetS-MCI
SA451116	NC-045	plasma	MetS-MCI
SA451117	NC-062	plasma	MetS-MCI

Showing results 1 to 95 of 95

Showing results 1 to 95 of 95

Collection:

Collection ID:	CO004077
Collection Summary:	Blood was collected in EDTA-coated tubes and centrifuged at 3,000 rpm to obtain plasma. The plasma was then transferred into cryovials and stored at –85°C until use.
Sample Type:	Blood (plasma)

Treatment:

Treatment ID:	TR004093
Treatment Summary:	All patients were divided into two groups: 1) MetS patients with MCI and 2) MetS patients with normal cognitive function. MCI was determined when the MoCA score was less than 23.

Sample Preparation:

Sampleprep ID:	SP004090
Sampleprep Summary:	The sample preparation method was modified from a previously published protocol (Jariyasopit et. al. (2021) J. Food Composition. Anal. vol 97, 103785. https://doi.org/10.1016/j.jfca.2020.103785). To convert fatty acids to FAMEs, a 50-µL aliquot of a plasma sample was mixed in a pyrex tube with 0.5 mL of borontrifluoride and nonadecanoate (final concentration of 20 ng µL-1) which was used as a surrogate. The sample mixture was heated at 100ºC for 1 h. After heating, the extract was allowed to cool down to room temperature before adding 1 mL n-hexane and brief vortexing. One milliliter of Milli-Q water was then added to the extract followed by vortexing for 20 s. The extract was centrifuged at 1,847 ×g, 20ºC for 15 min. The supernatant was dried under N2 stream and reconstituted in 500 µL hexane containing nonanoate (final concentration of 20 ng µL-1), used as an internal standard.

Sampleprep ID:

SP004090

Sampleprep Summary:

The sample preparation method was modified from a previously published protocol (Jariyasopit et. al. (2021) J. Food Composition. Anal. vol 97, 103785. https://doi.org/10.1016/j.jfca.2020.103785). To convert fatty acids to FAMEs, a 50-µL aliquot of a plasma sample was mixed in a pyrex tube with 0.5 mL of borontrifluoride and nonadecanoate (final concentration of 20 ng µL-1) which was used as a surrogate. The sample mixture was heated at 100ºC for 1 h. After heating, the extract was allowed to cool down to room temperature before adding 1 mL n-hexane and brief vortexing. One milliliter of Milli-Q water was then added to the extract followed by vortexing for 20 s. The extract was centrifuged at 1,847 ×g, 20ºC for 15 min. The supernatant was dried under N2 stream and reconstituted in 500 µL hexane containing nonanoate (final concentration of 20 ng µL-1), used as an internal standard.

Chromatography:

Chromatography ID:	CH004930
Chromatography Summary:	Determination of FAMEs was carried out using a GC-TOFMS (Pegasus-BT, Leco Corp., St.Joseph, MI). Target FAMEs were separated on a DB-FastFAME (30 m × 0.25 mm I.D., 0.25 µm film thickness, Agilent Technologies, U.S.A.). The injection volume was 1 µL. The samples were analyzed in split mode using the split ratio of 40:1. The injector temperature was kept at 200ºC. Helium was used as a carrier gas at a constant flow rate of 1 mL min-1. The GC oven temperature program started at 40ºC (2 min hold), increased to 150 ºC at 20 ºC min-1 (2 min hold), increased to 180ºC at 10ºC min-1 (3 min hold), increased to 190ºC at 5ºC min-1 (2 min hold), increased to 210ºC at 5ºC min-1, and increased to 240ºC at 15ºC min-1 (3 min hold). Transfer line and EI ion source temperatures were both kept at 250ºC.
Instrument Name:	Agilent 7890B
Column Name:	Agilent DB-FastFAME (30 m, 0.25 mm, 0.25 um)
Column Temperature:	programmed
Flow Gradient:	n/a
Flow Rate:	1 mL/min
Solvent A:	n/a
Solvent B:	n/a
Chromatography Type:	GC

Analysis:

Analysis ID:	AN006488
Analysis Type:	MS
Chromatography ID:	CH004930
Num Factors:	2
Num Metabolites:	10
Units:	µmol/dL plasma