Summary of Study ST004023

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002516. The data can be accessed directly via it's Project DOI: 10.21228/M8DZ7X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004023
Study TitleUntargeted Metabolomics Profiling of Human Plasma via LC-Q-TOF for sudden sensorineural hearing loss (SSNHL) Biomarker Discovery
Study Summary1. Background: Sudden sensorineural hearing loss (SSNHL) is a common otological disorder with complex etiologies and unclear pathophysiology. This study aimed to detect plasma metabolites in SSNHL, identify potential biomarkers, and uncover underlying metabolic mechanisms. 2. Methods: Participants: 64 SSNHL patients and 53 normal controls. Analysis: Plasma samples were analyzed by liquid chromatography-mass spectrometry (LC-MS). Metabolite profiling and multivariate statistical analyses (OPLS-DA, PLS-DA) were performed to screen differentially expressed metabolites. 3. Key Findings: Differential Metabolites: 130 metabolites were identified as significantly altered in SSNHL, primarily including: Lipids and lipid-like molecules (e.g., sphingosine), Amino acids and derivatives (e.g., anthranilic acid), Flavonoids (e.g., 6-hydroxyflavanone), Organic oxygen compounds involved in central carbon metabolism. Pathway Analysis: Enriched pathways included central carbon metabolism, protein digestion/absorption, aminoacyl-tRNA biosynthesis, and mineral absorption. 4. Conclusion: Plasma metabolite profiling shows promise as a non-invasive approach for SSNHL biomarker screening. Identified metabolites and pathways provide novel insights into SSNHL pathophysiology, potentially guiding future therapeutic strategies.
Institute
The First Affiliated Hospital of Kunming Medical University
Last NameZhang
First NameHuangruici
AddressNo. 295 Xichang Road, Kunming, Yunnan, 650032, China
Email1031784375@qq.com
Phone15508893380
Submit Date2025-06-17
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2025-07-28
Release Version1
Huangruici Zhang Huangruici Zhang
https://dx.doi.org/10.21228/M8DZ7X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002516
Project DOI:doi: 10.21228/M8DZ7X
Project Title:Untargeted Metabolomics Profiling of Human Plasma via LC-Q-TOF for sudden sensorineural hearing loss (SSNHL) Biomarker Discovery
Project Type:Untargeted Metabolomics
Project Summary:This project focuses on untargeted metabolomic analysis of human plasma samples using advanced mass spectrometry techniques (LC-MS). The goal is to (1) characterize the metabolic profile of human plasma; (2) identify potential biomarkers or metabolic pathways associated with sudden sensorineural hearing loss (SSNHL); (3) provide foundational metabolomic data for further biomedical research. Raw data, analytical methods, and metabolite annotations are submitted for transparency and reproducibility. In this study, non-targeted metabolomic analysis revealed that the differentially expressed metabolites in plasma of SSNHL patients mainly focus on lipid and lipid-like molecules, amino acids and their derivatives, flavonoids, as well as organic oxygen compounds involved in central carbon metabolism and energy metabolic pathways such as tricarboxylic acid cycle intermediates.
Institute:The First Affiliated Hospital of Kunming Medical University
Last Name:Zhang
First Name:Huangruici
Address:No. 295 Xichang Road, Kunming, Yunnan, 650032, China
Email:1031784375@qq.com
Phone:15508893380

Subject:

Subject ID:SU004161
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Treatment
SA462620TX-61plasma case
SA462621TX-64plasma case
SA462622TX-63plasma case
SA462623TX-62plasma case
SA462624TX-1plasma case
SA462625TX-2plasma case
SA462626TX-16plasma case
SA462627TX-28plasma case
SA462628TX-27plasma case
SA462629TX-26plasma case
SA462630TX-25plasma case
SA462631TX-24plasma case
SA462632TX-23plasma case
SA462633TX-22plasma case
SA462634TX-21plasma case
SA462635TX-20plasma case
SA462636TX-19plasma case
SA462637TX-18plasma case
SA462638TX-17plasma case
SA462639TX-15plasma case
SA462640TX-30plasma case
SA462641TX-14plasma case
SA462642TX-13plasma case
SA462643TX-12plasma case
SA462644TX-11plasma case
SA462645TX-10plasma case
SA462646TX-9plasma case
SA462647TX-8plasma case
SA462648TX-7plasma case
SA462649TX-6plasma case
SA462650TX-5plasma case
SA462651TX-4plasma case
SA462652TX-3plasma case
SA462653TX-29plasma case
SA462654TX-31plasma case
SA462655TX-59plasma case
SA462656TX-46plasma case
SA462657TX-58plasma case
SA462658TX-57plasma case
SA462659TX-56plasma case
SA462660TX-55plasma case
SA462661TX-54plasma case
SA462662TX-53plasma case
SA462663TX-52plasma case
SA462664TX-51plasma case
SA462665TX-50plasma case
SA462666TX-49plasma case
SA462667TX-48plasma case
SA462668TX-47plasma case
SA462669TX-45plasma case
SA462670TX-32plasma case
SA462671TX-44plasma case
SA462672TX-43plasma case
SA462673TX-42plasma case
SA462674TX-41plasma case
SA462675TX-40plasma case
SA462676TX-39plasma case
SA462677TX-38plasma case
SA462678TX-37plasma case
SA462679TX-36plasma case
SA462680TX-35plasma case
SA462681TX-34plasma case
SA462682TX-33plasma case
SA462683TX-60plasma case
SA462567CKX-13plasma Control
SA462568CKX-24plasma Control
SA462569CKX-23plasma Control
SA462570CKX-22plasma Control
SA462571CKX-21plasma Control
SA462572CKX-20plasma Control
SA462573CKX-19plasma Control
SA462574CKX-18plasma Control
SA462575CKX-17plasma Control
SA462576CKX-16plasma Control
SA462577CKX-15plasma Control
SA462578CKX-14plasma Control
SA462579CKX-12plasma Control
SA462580CKX-27plasma Control
SA462581CKX-11plasma Control
SA462582CKX-10plasma Control
SA462583CKX-9plasma Control
SA462584CKX-8plasma Control
SA462585CKX-7plasma Control
SA462586CKX-6plasma Control
SA462587CKX-5plasma Control
SA462588CKX-4plasma Control
SA462589CKX-3plasma Control
SA462590CKX-2plasma Control
SA462591CKX-1plasma Control
SA462592CKX-25plasma Control
SA462593CKX-26plasma Control
SA462594CKX-28plasma Control
SA462595CKX-41plasma Control
SA462596CKX-53plasma Control
SA462597CKX-52plasma Control
SA462598CKX-51plasma Control
SA462599CKX-50plasma Control
SA462600CKX-49plasma Control
SA462601CKX-48plasma Control
SA462602CKX-47plasma Control
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Collection:

Collection ID:CO004154
Collection Summary:Fasting blood samples from control (non-SSNHL) and case (SSNHL) individuals were collected in 5 mL of vacuum blood collection tube containing EDTA anticoagulant, centrifuged at 1500g at 4°C for 15 minutes, and 150 μL of plasma were taken and stored at -80°C. After thawing at 4°C before detection, protein was precipitated with cold methanol/acetonitrile/water (2:2:1, v/v) and metabolites were extracted.
Collection Protocol Filename:ZHRC_Method.pdf
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR004170
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP004167
Sampleprep Summary:Plasma samples were collected from 64 sudden sensorineural hearing loss (SSNHL) patients and 53 healthy controls into EDTA-anticoagulated tubes after an overnight fast. Samples were centrifuged at 1500 × g for 15 min at 4°C, and 150 μL plasma aliquots were stored at –80°C. For metabolomic extraction, 100 μL thawed plasma was mixed with 400 μL cold methanol/acetonitrile (1:1, v/v), vortexed, sonicated for 30 min at low temperature, and incubated at –20°C for 10 min. The mixture was centrifuged at 14,000 × g for 20 min at 4°C, and the supernatant was vacuum-dried. The dried pellet was re-dissolved in 100 μL acetonitrile/water (1:1, v/v), centrifuged again at 14,000 × g for 15 min, and the supernatant was injected into the UHPLC-MS system. Quality control (QC) samples were prepared by pooling 10 μL of each plasma sample and inserted every 5 samples to monitor analytical reproducibility, validated via TIC overlap, PCA clustering, and RSD ≤ 30% for >80% of ion peaks. Total sum normalization was applied to preprocess data for inter-sample comparability.
Sampleprep Protocol Filename:ZHRC_Method.pdf

Chromatography:

Chromatography ID:CH005038
Chromatography Summary:Ultra-high performance liquid chromatography (UHPLC) combined with hydrophilic interaction chromatography (HILIC) for plasma metabolite separation, using gradient elution and hyphenated with high-resolution mass spectrometry.
Methods Filename:ZHRC_Method.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:25
Flow Gradient:0–0.5 min, 95% B; 0.5–7 min, B linearly decreased to 65%; 7–8 min, B decreased to 40%; 8–9 min, hold at 40%; 9–9.1 min, B increased to 95%; 9.1–12 min, hold at 95%
Flow Rate:0.5 mL/min
Solvent A:100% Water; 25 mM ammonium acetate; 25 mM ammonium hydroxide
Solvent B:100% Acetonitrile
Chromatography Type:HILIC
  
Chromatography ID:CH005039
Chromatography Summary:Ultra-high performance liquid chromatography (UHPLC) combined with hydrophilic interaction chromatography (HILIC) for plasma metabolite separation, using gradient elution and hyphenated with high-resolution mass spectrometry.
Methods Filename:ZHRC_Method.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:25
Flow Gradient:0–1.5 min, 98% B; 1.5–12 min, B decreased to 2%; 12–14 min, hold at 2%; 14–14.1 min, B increased to 98%; 14.1–17 min, hold at 98%.
Flow Rate:0.3 mL/min
Solvent A:100% Water; 25 mM ammonium acetate; 25 mM ammonium hydroxide
Solvent B:100% Acetonitrile
Chromatography Type:HILIC

Analysis:

Analysis ID:AN006629
Analysis Type:MS
Analysis Protocol File:ZHRC_Method.pdf
Chromatography ID:CH005038
Has Mz:1
Has Rt:1
Rt Units:Seconds
Results File:ST004023_AN006629_Results.txt
Units:Peak area
  
Analysis ID:AN006630
Analysis Type:MS
Analysis Protocol File:ZHRC_Method.pdf
Chromatography ID:CH005039
Has Mz:1
Has Rt:1
Rt Units:Seconds
Results File:ST004023_AN006630_Results.txt
Units:Peak area
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