Summary of Study ST004034

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002526. The data can be accessed directly via it's Project DOI: 10.21228/M84G0Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples | Perform analysis on untargeted data
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST004034
Study Title	A Multi-Tissue Lipidome and Adipose Proteome Atlas Reveals Perinatal Bisphenol S Exposure Primes Offspring for High-Fat Diet-Induced Obesity and Insulin Resistance
Study Summary	Due to increasingly strict regulations on the use of bisphenol A (BPA), its structurally similar alternative, bisphenol S (BPS), has been widely used as a major substitute in many industries, including food packaging. However, recent studies have revealed that BPS may promote obesity and lead to insulin resistance, especially in the early stages of life, although its exact impact is not yet fully understood. To address these concerns, this study established a mouse model to explore the exposure of female mice to BPS during pregnancy and lactation, as well as the consumption of high-fat diets by their offspring. By evaluating the biological phenotypes related to obesity and insulin resistance in the offspring mice, multi-tissue lipidomics analysis and adipose tissue proteomics analysis were carried out. Ultimately, a correlation study was conducted by combining phenotypes, lipidomics, and proteomics to explore the risks and potential mechanisms of BPS exposure to obesity and insulin resistance.
Institute	Jimei University
Last Name	Li
First Name	Shuyin
Address	No.185 Yinjiang Road,Jimei District,Xiamen City,Fujian Province,China
Email	164000107@qq.com
Phone	18750682266
Submit Date	2025-07-08
Raw Data Available	Yes
Raw Data File Type(s)	mzML, raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2025-07-31
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002526
Project DOI:	doi: 10.21228/M84G0Z
Project Title:	A Multi-Tissue Lipidome and Adipose Proteome Atlas Reveals Perinatal Bisphenol S Exposure Primes Offspring for High-Fat Diet-Induced Obesity and Insulin Resistance
Project Summary:	Due to increasingly strict regulations on the use of bisphenol A (BPA), its structurally similar alternative, bisphenol S (BPS), has been widely used as a major substitute in many industries, including food packaging. However, recent studies have revealed that BPS may promote obesity and lead to insulin resistance, especially in the early stages of life, although its exact impact is not yet fully understood. To address these concerns, this study established a mouse model to explore the exposure of female mice to BPS during pregnancy and lactation, as well as the consumption of high-fat diets by their offspring. By evaluating the biological phenotypes related to obesity and insulin resistance in the offspring mice, multi-tissue lipidomics analysis and adipose tissue proteomics analysis were carried out. Ultimately, a correlation study was conducted by combining phenotypes, lipidomics, and proteomics to explore the risks and potential mechanisms of BPS exposure to obesity and insulin resistance.
Institute:	Jimei University
Last Name:	Li
First Name:	Shuyin
Address:	No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
Email:	164000107@qq.com
Phone:	18750682266

Subject:

Subject ID:	SU004180
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	BPS Treatment	Sample source
SA465801	BLF-267	0.05mg/kg/d	Brown Adipose Tissue
SA465802	BLF-258	0.05mg/kg/d	Brown Adipose Tissue
SA465803	BLF-261	0.05mg/kg/d	Brown Adipose Tissue
SA465804	BLF-262	0.05mg/kg/d	Brown Adipose Tissue
SA465805	BLF-263	0.05mg/kg/d	Brown Adipose Tissue
SA465806	BLF-266	0.05mg/kg/d	Brown Adipose Tissue
SA465807	BLF-264	0.05mg/kg/d	Brown Adipose Tissue
SA465808	BLM-247	0.05mg/kg/d	Brown Adipose Tissue
SA465809	BLM-252	0.05mg/kg/d	Brown Adipose Tissue
SA465810	BLM-253	0.05mg/kg/d	Brown Adipose Tissue
SA465811	BLM-255	0.05mg/kg/d	Brown Adipose Tissue
SA465812	BLM-275	0.05mg/kg/d	Brown Adipose Tissue
SA465813	BLM-278	0.05mg/kg/d	Brown Adipose Tissue
SA465814	BLM-279	0.05mg/kg/d	Brown Adipose Tissue
SA465815	HLM-247	0.05mg/kg/d	Heart Tissue
SA465816	HLF-258	0.05mg/kg/d	Heart Tissue
SA465817	HLF-261	0.05mg/kg/d	Heart Tissue
SA465818	HLF-262	0.05mg/kg/d	Heart Tissue
SA465819	HLF-264	0.05mg/kg/d	Heart Tissue
SA465820	HLF-266	0.05mg/kg/d	Heart Tissue
SA465821	HLF-267	0.05mg/kg/d	Heart Tissue
SA465822	HLF-263	0.05mg/kg/d	Heart Tissue
SA465823	HLM-252	0.05mg/kg/d	Heart Tissue
SA465824	HLM-255	0.05mg/kg/d	Heart Tissue
SA465825	HLM-275	0.05mg/kg/d	Heart Tissue
SA465826	HLM-278	0.05mg/kg/d	Heart Tissue
SA465827	HLM-279	0.05mg/kg/d	Heart Tissue
SA465828	HLM-253	0.05mg/kg/d	Heart Tissue
SA465829	LLM-247	0.05mg/kg/d	Liver Tissue
SA465830	LLM-279	0.05mg/kg/d	Liver Tissue
SA465831	LLM-255	0.05mg/kg/d	Liver Tissue
SA465832	LLM-253	0.05mg/kg/d	Liver Tissue
SA465833	LLM-252	0.05mg/kg/d	Liver Tissue
SA465834	LLF-264	0.05mg/kg/d	Liver Tissue
SA465835	LLF-267	0.05mg/kg/d	Liver Tissue
SA465836	LLF-266	0.05mg/kg/d	Liver Tissue
SA465837	LLM-275	0.05mg/kg/d	Liver Tissue
SA465838	LLF-263	0.05mg/kg/d	Liver Tissue
SA465839	LLF-262	0.05mg/kg/d	Liver Tissue
SA465840	LLF-261	0.05mg/kg/d	Liver Tissue
SA465841	LLF-258	0.05mg/kg/d	Liver Tissue
SA465842	LLM-278	0.05mg/kg/d	Liver Tissue
SA465843	PLM-255	0.05mg/kg/d	Pancreas Tissue
SA465844	PLM-275	0.05mg/kg/d	Pancreas Tissue
SA465845	PLM-253	0.05mg/kg/d	Pancreas Tissue
SA465846	PLM-252	0.05mg/kg/d	Pancreas Tissue
SA465847	PLM-247	0.05mg/kg/d	Pancreas Tissue
SA465848	PLF-267	0.05mg/kg/d	Pancreas Tissue
SA465849	PLF-266	0.05mg/kg/d	Pancreas Tissue
SA465850	PLF-264	0.05mg/kg/d	Pancreas Tissue
SA465851	PLF-263	0.05mg/kg/d	Pancreas Tissue
SA465852	PLF-258	0.05mg/kg/d	Pancreas Tissue
SA465853	PLF-261	0.05mg/kg/d	Pancreas Tissue
SA465854	PLM-279	0.05mg/kg/d	Pancreas Tissue
SA465855	PLM-278	0.05mg/kg/d	Pancreas Tissue
SA465856	PLF-262	0.05mg/kg/d	Pancreas Tissue
SA465857	WLF-258	0.05mg/kg/d	White Adipose Tissue
SA465858	WLM-247	0.05mg/kg/d	White Adipose Tissue
SA465859	WLF-262	0.05mg/kg/d	White Adipose Tissue
SA465860	WLF-263	0.05mg/kg/d	White Adipose Tissue
SA465861	WLF-264	0.05mg/kg/d	White Adipose Tissue
SA465862	WLF-266	0.05mg/kg/d	White Adipose Tissue
SA465863	WLF-267	0.05mg/kg/d	White Adipose Tissue
SA465864	WLF-261	0.05mg/kg/d	White Adipose Tissue
SA465865	WLM-252	0.05mg/kg/d	White Adipose Tissue
SA465866	WLM-255	0.05mg/kg/d	White Adipose Tissue
SA465867	WLM-275	0.05mg/kg/d	White Adipose Tissue
SA465868	WLM-278	0.05mg/kg/d	White Adipose Tissue
SA465869	WLM-279	0.05mg/kg/d	White Adipose Tissue
SA465870	WLM-253	0.05mg/kg/d	White Adipose Tissue
SA465871	BHF-4417	5mg/kg/d	Brown Adipose Tissue
SA465872	BHM-294	5mg/kg/d	Brown Adipose Tissue
SA465873	BHM-290	5mg/kg/d	Brown Adipose Tissue
SA465874	BHM-289	5mg/kg/d	Brown Adipose Tissue
SA465875	BHF-4438	5mg/kg/d	Brown Adipose Tissue
SA465876	BHF-4419	5mg/kg/d	Brown Adipose Tissue
SA465877	BHF-4418	5mg/kg/d	Brown Adipose Tissue
SA465878	BHF-307	5mg/kg/d	Brown Adipose Tissue
SA465879	BHF-4416	5mg/kg/d	Brown Adipose Tissue
SA465880	BHF-4415	5mg/kg/d	Brown Adipose Tissue
SA465881	BHF-4413	5mg/kg/d	Brown Adipose Tissue
SA465882	BHF-306	5mg/kg/d	Brown Adipose Tissue
SA465883	BHF-299	5mg/kg/d	Brown Adipose Tissue
SA465884	BHF-298	5mg/kg/d	Brown Adipose Tissue
SA465885	BHM-301	5mg/kg/d	Brown Adipose Tissue
SA465886	BHF-295	5mg/kg/d	Brown Adipose Tissue
SA465887	BHF-297	5mg/kg/d	Brown Adipose Tissue
SA465888	BHM-300	5mg/kg/d	Brown Adipose Tissue
SA465889	BHM-305	5mg/kg/d	Brown Adipose Tissue
SA465890	BHM-4452	5mg/kg/d	Brown Adipose Tissue
SA465891	BHM-4434	5mg/kg/d	Brown Adipose Tissue
SA465892	BHM-4433	5mg/kg/d	Brown Adipose Tissue
SA465893	BHM-4432	5mg/kg/d	Brown Adipose Tissue
SA465894	BHM-4431	5mg/kg/d	Brown Adipose Tissue
SA465895	BHM-4427	5mg/kg/d	Brown Adipose Tissue
SA465896	BHM-4423	5mg/kg/d	Brown Adipose Tissue
SA465897	BHM-304	5mg/kg/d	Brown Adipose Tissue
SA465898	HHF-299	5mg/kg/d	Heart Tissue
SA465899	HHF-307	5mg/kg/d	Heart Tissue
SA465900	HHF-306	5mg/kg/d	Heart Tissue

Showing page 1 of 3 Results: 1 2 3 Next Showing results 1 to 100 of 275

Collection:

Collection ID:	CO004173
Collection Summary:	After three weeks of feeding, adaptive feed was given for one week, followed by high-fat feed for eight weeks. The method of euthanasia for mice caused by cervical dislocation. Take the heart, liver, pancreas, white fat and brown fat of mice. After sampling, quickly rinse the tissue with normal saline. The cleaned tissues were treated with liquid nitrogen and finally frozen in a -80℃ refrigerator.
Sample Type:	White adipose,Brown adipose,Heart,Liver,Pancreas

Treatment:

Treatment ID:	TR004189
Treatment Summary:	Simply put, C57BL/6 pregnant mice were fed a normal diet under constant temperature [(23±1) ℃] and constant humidity [(55±5)%] conditions, and strictly followed a 12-hour / 12-hour light-dark cycle. In the experiment, the animals were kept in polystyrene mouse cages. Mice were divided into the normal saline solvent control group, the low-dose BPS group (0.05 mg/kg/d), and the high-dose BPS group (5 mg/kg/d). BPS exposure is performed through subcutaneous injection. Observe the general condition of pregnant mice every day and measure their weight once a week. After delivery, pregnant mice in different groups were exposed to BPS during lactation, and the female mice were sacrificed after lactation. After lactation, observe the general condition of F1 mice every day and weigh them once a week. After three weeks of feeding, adaptive feed was given for one week, followed by high-fat feed for eight weeks. Collect the hearts, livers, pancreas, white fat and brown fat of mice. After sampling, the mice were quickly washed with normal saline. After cleaning, the mice were treated with liquid nitrogen and finally frozen in a refrigerator at -80℃.

Treatment ID:

TR004189

Treatment Summary:

Simply put, C57BL/6 pregnant mice were fed a normal diet under constant temperature [(23±1) ℃] and constant humidity [(55±5)%] conditions, and strictly followed a 12-hour / 12-hour light-dark cycle. In the experiment, the animals were kept in polystyrene mouse cages. Mice were divided into the normal saline solvent control group, the low-dose BPS group (0.05 mg/kg/d), and the high-dose BPS group (5 mg/kg/d). BPS exposure is performed through subcutaneous injection. Observe the general condition of pregnant mice every day and measure their weight once a week. After delivery, pregnant mice in different groups were exposed to BPS during lactation, and the female mice were sacrificed after lactation. After lactation, observe the general condition of F1 mice every day and weigh them once a week. After three weeks of feeding, adaptive feed was given for one week, followed by high-fat feed for eight weeks. Collect the hearts, livers, pancreas, white fat and brown fat of mice. After sampling, the mice were quickly washed with normal saline. After cleaning, the mice were treated with liquid nitrogen and finally frozen in a refrigerator at -80℃.

Sample Preparation:

Sampleprep ID:	SP004186
Sampleprep Summary:	Each repeated mouse tissue sample (~15 mg) was precisely weighed and transferred to 2 mL Eppendorf centrifuge tubes. Subsequently, magnetic beads and 400 μL of pre-cooled methanol solution containing internal standards were added in sequence. Internal standards was added, consisting of 1 μg/mL FFA (C16:0)-d3 and FFA (C18:0)-d3, 1.63 μg/mL LPC (19:0) ,1.30 μg/mL SM (d18:1/12:0), 1.88 μg/mL PC (38: 0),1.38 μg/mL PE (30:0), 1.26 μg/mL TG (45:0), and 1.27 μg/mL Cer (d18:1/17:0). Then, tissue homogenization was performed at 65 Hz for 1 minute using a high-throughput rapid grinder. The mouse heart, liver and pancreatic tissues were ground and homogenized twice. The white fat and brown fat of mice were ground and homogenized four times. After grinding is completed, add 1mL of pre-cooled methyl tert-butyl ether to a centrifuge tube, vortex and mix for 30 seconds first. Then, use a constant temperature mixer set to 10℃ and vortex at 1000 rpm for 30 minutes. Subsequently, add 400 μL of pre-cooled ultrapure water, continue to vortex and mix for 1 minute, and let it stand at 6℃ for 5 minutes. The upper and lower phases were stratified by centrifugation (15000 g, 15 min, 6℃). Collect the upper hydrophobic phase into 1.5 mL Eppendorf centrifuge tubes, then place the samples in a freeze vacuum centrifugal concentrator for low-temperature drying treatment. Finally, store the obtained samples in a -80°C refrigerator for subsequent analysis.

Sampleprep ID:

SP004186

Sampleprep Summary:

Each repeated mouse tissue sample (~15 mg) was precisely weighed and transferred to 2 mL Eppendorf centrifuge tubes. Subsequently, magnetic beads and 400 μL of pre-cooled methanol solution containing internal standards were added in sequence. Internal standards was added, consisting of 1 μg/mL FFA (C16:0)-d3 and FFA (C18:0)-d3, 1.63 μg/mL LPC (19:0) ,1.30 μg/mL SM (d18:1/12:0), 1.88 μg/mL PC (38: 0),1.38 μg/mL PE (30:0), 1.26 μg/mL TG (45:0), and 1.27 μg/mL Cer (d18:1/17:0). Then, tissue homogenization was performed at 65 Hz for 1 minute using a high-throughput rapid grinder. The mouse heart, liver and pancreatic tissues were ground and homogenized twice. The white fat and brown fat of mice were ground and homogenized four times. After grinding is completed, add 1mL of pre-cooled methyl tert-butyl ether to a centrifuge tube, vortex and mix for 30 seconds first. Then, use a constant temperature mixer set to 10℃ and vortex at 1000 rpm for 30 minutes. Subsequently, add 400 μL of pre-cooled ultrapure water, continue to vortex and mix for 1 minute, and let it stand at 6℃ for 5 minutes. The upper and lower phases were stratified by centrifugation (15000 g, 15 min, 6℃). Collect the upper hydrophobic phase into 1.5 mL Eppendorf centrifuge tubes, then place the samples in a freeze vacuum centrifugal concentrator for low-temperature drying treatment. Finally, store the obtained samples in a -80°C refrigerator for subsequent analysis.

Chromatography:

Chromatography ID:	CH005068
Chromatography Summary:	Positive
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Waters ACQUITY UPLC BEH C8 (100 x 2.1 mm, 1.7 μm)
Column Temperature:	55°C
Flow Gradient:	First, maintain 32% B for 1.5 minutes, then linearly increase it from 1.5 to 15.5 minutes to 85% B, then linearly increase it to 97% B within 0.1 minutes, maintain it until 18 minutes, and finally rapidly decrease it to 32% B at 0.1 minutes, and equilibrium until the next injection.
Flow Rate:	0.26 mL/min
Solvent A:	60% Acetonitrile/40% Water; 10 mM Ammonium Acetate
Solvent B:	90% Isopropyl alcohol/10% Acetonitrile; 10 mM Ammonium Acetate
Chromatography Type:	Reversed phase

Chromatography ID:	CH005069
Chromatography Summary:	Negative
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Waters ACQUITY UPLC BEH C8 (100 x 2.1 mm, 1.7 μm)
Column Temperature:	55°C
Flow Gradient:	First, maintain 32% B for 1.5 minutes, then linearly increase it from 1.5 to 15.5 minutes to 85% B, then linearly increase it to 97% B within 0.1 minutes, maintain it until 18 minutes, and finally rapidly decrease it to 32% B at 0.1 minutes, and equilibrium until the next injection.
Flow Rate:	0.26 mL/min
Solvent A:	60% Acetonitrile/40% Water; 10 mM Ammonium Acetate
Solvent B:	90% Isopropyl alcohol/10% Acetonitrile; 10 mM Ammonium Acetate
Chromatography Type:	Reversed phase

Analysis:

Analysis ID:	AN006671
Analysis Type:	MS
Software Version:	Trace Finder(Thermo, USA)
Operator Name:	Shuyin Li
Detector Type:	Q-Exactive，Thermo Fisher，USA
Data Format:	txt
Chromatography ID:	CH005068
Has Mz:	1
Has Rt:	1
Rt Units:	Minutes
Results File:	ST004034_AN006671_Results.txt
Units:	Peak area

Analysis ID:	AN006672
Analysis Type:	MS
Software Version:	Trace Finder(Thermo, USA)
Operator Name:	Shuyin Li
Detector Type:	Q-Exactive，Thermo Fisher，USA
Data Format:	txt
Chromatography ID:	CH005069
Has Mz:	1
Has Rt:	1
Rt Units:	Minutes
Results File:	ST004034_AN006672_Results.txt
Units:	Peak area