Summary of Study ST004036

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002528. The data can be accessed directly via it's Project DOI: 10.21228/M8VZ56 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004036
Study TitleMetabolome Changes of Megachile rotundata Across Pupal Stages Measured by Mass Spectrometry-Based Untargeted Metabolomics
Study TypeDiscovery-based
Study SummaryMegachile rotundata pupal stages Prepupae (PP), Red Eye (RE), and Melanized (M) showed distinguishable metabolite profiles as measured by untargeted GCMS analysis. Metabolites associated with the different growth stages include amino acids, neurotransmitters, sugars, sugar phosphates, amino sugars, and sugar alcohols. Metabolic profiles between sexes within each growth stage were indistinguishable as measured in this study.
Institute
University of Wyoming
DepartmentDepartment of Chemistry
Last NameFranco
First NameBasile
Address1000 E. University Ave (3838) Dept. of Chemistry
Emailbasile@uwyo.edu
Phone3077664376
Submit Date2025-06-27
Num Groups3
Total Subjects62
Num Males23
Num Females17
Study Commentssex determination at the pupae stages is ambiguous
Publicationsin preparation
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2025-07-31
Release Version1
Basile Franco Basile Franco
https://dx.doi.org/10.21228/M8VZ56
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002528
Project DOI:doi: 10.21228/M8VZ56
Project Title:Metabolome Changes of Megachile rotundata Across Pupal Stages Measured by Mass Spectrometry-Based Untargeted Metabolomics
Project Type:untargeted metabolomics
Project Summary:Untargeted metabolomics measurements were conducted via Gas Chromatography-Mass Spectrometry (GC-MS) on both sexes, male and female, of the alfalfa leaf cutting bee, Megachile rotundata during three stages: Prepupae (PP), Red Eye Pupae (RE), and Melanized Pupae (ME). Focusing on the analysis of polar metabolites, results uncovered clear metabolic differences between the three stages of M. rotundata. However, metabolic profiles between sexes within each growth stage were indistinguishable. A total of 289 metabolite entities or features were tentatively identified between the three pupal stages studied.
Institute:University of Wyoming
Department:Department of Chemistry
Last Name:Basile
First Name:Franco
Address:1000 E. University Ave, Chemistry Department 3838
Email:basile@uwyo.edu
Phone:3034780236
Funding Source:NSF
Publications:in preparation
Contributors:Kendra J. Greenlee (U. North Dakota), Joseph P. Rinehart (USDA)

Subject:

Subject ID:SU004182
Subject Type:Insect
Subject Species:Megachile rotundata
Taxonomy ID:143995
Age Or Age Range:pupae stages
Gender:Male and female

Factors:

Subject type: Insect; Subject species: Megachile rotundata (Factor headings shown in green)

mb_sample_id local_sample_id pupae growth stage Sample source
SA466116MM2melanized pupae tissue
SA466117MF2melanized pupae tissue
SA466118MM9melanized pupae tissue
SA466119MM8melanized pupae tissue
SA466120MM7melanized pupae tissue
SA466121MM6melanized pupae tissue
SA466122MM5melanized pupae tissue
SA466123MM4melanized pupae tissue
SA466124MM3melanized pupae tissue
SA466125MF1melanized pupae tissue
SA466126MM13melanized pupae tissue
SA466127MM12melanized pupae tissue
SA466128MM11melanized pupae tissue
SA466129MM10melanized pupae tissue
SA466130MM1melanized pupae tissue
SA466131MF7melanized pupae tissue
SA466132MF6melanized pupae tissue
SA466133MF5melanized pupae tissue
SA466134MF4melanized pupae tissue
SA466135MF3melanized pupae tissue
SA466136PP21prepupae pupae tissue
SA466137PP22prepupae pupae tissue
SA466138PP3prepupae pupae tissue
SA466139PP9prepupae pupae tissue
SA466140PP4prepupae pupae tissue
SA466141PP6prepupae pupae tissue
SA466142PP7prepupae pupae tissue
SA466143PP8prepupae pupae tissue
SA466144PP20prepupae pupae tissue
SA466145PP5prepupae pupae tissue
SA466146PP19prepupae pupae tissue
SA466147PP12prepupae pupae tissue
SA466148PP18prepupae pupae tissue
SA466149PP1prepupae pupae tissue
SA466150PP10prepupae pupae tissue
SA466151PP11prepupae pupae tissue
SA466152PP2prepupae pupae tissue
SA466153PP13prepupae pupae tissue
SA466154PP15prepupae pupae tissue
SA466155PP16prepupae pupae tissue
SA466156PP17prepupae pupae tissue
SA466157PP14prepupae pupae tissue
SA466158REM10red-eyed pupae tissue
SA466159REM9red-eyed pupae tissue
SA466160REM8red-eyed pupae tissue
SA466161REM7red-eyed pupae tissue
SA466162REM6red-eyed pupae tissue
SA466163REM5red-eyed pupae tissue
SA466164REM4red-eyed pupae tissue
SA466165REM3red-eyed pupae tissue
SA466166REM2red-eyed pupae tissue
SA466167REF8red-eyed pupae tissue
SA466168REM1red-eyed pupae tissue
SA466169REF9red-eyed pupae tissue
SA466170REF7red-eyed pupae tissue
SA466171REF6red-eyed pupae tissue
SA466172REF4red-eyed pupae tissue
SA466173REF3red-eyed pupae tissue
SA466174REF2red-eyed pupae tissue
SA466175REF10red-eyed pupae tissue
SA466176REF1red-eyed pupae tissue
SA466177REF5red-eyed pupae tissue
Showing results 1 to 62 of 62

Collection:

Collection ID:CO004175
Collection Summary:Prepupae used in this study were purchased in April of 2020 (JWM Leafcutters, Nampa, ID) and stored upon arrival in Fargo (USDA-ARS Edward T. Schafer Agricultural Research Center) at 6°C, as previously described (Rinehart et al., 2011). To initiate development, bees were placed in 29°C with 75% humidity. Bees were sampled immediately prior to development, prepupae (PP), or allowed to develop to one of 2 growth stages of pupae classified as red-eye pupae (RE) or melanized pupae (ME).
Collection Protocol Filename:CollectionProtocol_Megachile_rotundata_pupae.pdf
Sample Type:Insect tissue
Collection Frequency:once
Storage Conditions:-80℃

Treatment:

Treatment ID:TR004191
Treatment Summary:No treatment. Different bee pupae growth stages were compared in this study. Bees were sampled immediately before development, prepupae (PP), or allowed to develop to one of 2 growth stages of pupae classified as red-eye pupae (RE) or melanized pupae (ME).
Treatment:No treatment. Different bee pupae growth stages were compared in this study.

Sample Preparation:

Sampleprep ID:SP004188
Sampleprep Summary:After the collection of bees, a biphasic extraction method (Matyash et al., 2008) using MTBE, methanol and water was implemented. The aqueous phase was processed and later analyzed by GC-MS.
Sampleprep Protocol Filename:SamplePreparation_Megachile_rotundata_pupae.pdf
Processing Storage Conditions:4℃
Extraction Method:MTBE extraction
Extract Storage:4℃
Sample Derivatization:methoximation (MeOX)-MSTFA
Sample Spiking:FAMEs

Chromatography:

Chromatography ID:CH005071
Chromatography Summary:The GC-MS method used in this study was adapted from Fiehn (Fiehn, 2016) with some modifications to optimize our specific GC-MS instrument (Pegasus HRT+).
Methods Filename:MethodsGCMS_Megachile_rotundata_pupae.pdf
Instrument Name:Agilent 7890B
Column Name:Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
Column Temperature:initial temperature of 60°C held for 1 minute, then ramped at 5°C/min to 330°C, and held for 10 minutes
Flow Gradient:NA
Flow Rate:Helium 1 mL/min
Injection Temperature:250
Retention Index:FAME
Sample Injection:splitless
Solvent A:NA
Solvent B:NA
Chromatography Type:GC

Analysis:

Analysis ID:AN006674
Laboratory Name:Basile Laboratory, Chemistry Dept, Univ. Wyoming
Analysis Type:MS
Analysis Protocol File:MethodsGCMS_Megachile_rotundata_pupae.pdf
Operator Name:Banani Mondal
Data Format:CDF
Chromatography ID:CH005071
Num Factors:3
Num Metabolites:285
Units:arbitrary units
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