Summary of Study ST004083

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002564. The data can be accessed directly via it's Project DOI: 10.21228/M8725N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004083
Study TitleDietary methionine restriction in high-fat diet-fed male mice, brown adipose tissue metabolomics
Study Summary25-week-old male mice (n=31) with diet-induced obesity were divided into four groups. The first group (n=8) was fed a high-fat diet (HFD, 60% kcal fat) for 15 weeks. The second, third, and fourth groups were fed a modified high-fat diet (HFD-MetR, 60% fat/0.12% methionine) for 3 days (MetR3, n=8), 5 days (MetR5, n=8), or 10 days (MetR10, n=7), respectively. The HFD group (60% fat/0.86% methionine) served as the control. Mice were fasted for 4 hours prior to sample collection. The goal of this study was to investigate the effect of methionine restriction on brown adipose tissues.
Institute
Panome Bio
Last NameGuzior
First NameDouglas
Address4340 Duncan Ave, St. Louis, MO 63110
Emaildouglas.guzior@panomebio.com
Phone(314) 632-6588
Submit Date2025-03-26
Num Groups4
Total Subjects31
Num Males31
Study CommentsPart 1 of 3
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-08-25
Release Version1
Douglas Guzior Douglas Guzior
https://dx.doi.org/10.21228/M8725N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002564
Project DOI:doi: 10.21228/M8725N
Project Title:Short-term restriction of dietary methionine counteracts metabolic dysfunction in obese mice fed a high fat diet
Project Summary:Twenty-five-week-old male mice (n=31) with diet-induced obesity were divided into four groups to investigate the impact of methionine restriction on metabolic profiles within plasma, brown adipose tissue, and liver, aiming to determine if short-term dietary methionine restriction (MetR) could reverse the metabolic disruptions caused by a high-fat diet and to characterize the resulting alterations in protein and lipid signatures across these key metabolic tissues. The control group (n=8) was maintained on a high-fat diet (HFD, 60% kcal fat/0.86% methionine) for 15 weeks, while the intervention groups were fed a modified high-fat diet with significantly reduced methionine (HFD-MetR, 60% fat/0.12% methionine) for 3 days (MetR3, n=8), 5 days (MetR5, n=8), or 10 days (MetR10, n=7), with all mice fasted for 4 hours prior to the collection of biological samples for comprehensive metabolomic analysis. Our findings revealed that even short durations of HFD-MetR led to rapid and significant metabolic improvements, evidenced by reduced circulating triglyceride levels, blood glucose, and insulin within 10 days. Furthermore, significant changes in the secretion of adipokines and hepatokines were observed, alongside distinct alterations in the overall metabolomic profiles of plasma, brown adipose tissue, and liver. Proteomic analysis of perivascular adipose tissue (PVAT) complemented these findings by revealing enriched metabolic signatures. These data demonstrate that short-term dietary methionine restriction elicits rapid and substantial changes in the metabolome across multiple tissues in obese mice, highlighting its potential as a potent dietary intervention to reverse diet-induced metabolic dysfunction.
Institute:Panome Bio
Last Name:Guzior
First Name:Douglas
Address:4340 Duncan Ave, St. Louis, MO 63110
Email:douglas.guzior@panomebio.com
Phone:‪(314) 632-6588

Subject:

Subject ID:SU004229
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:25 wk
Weight Or Weight Range:>45g bodyweight at 25 w/o
Gender:Male
Animal Light Cycle:12-hour light/12-hour dark photoperiod (0700 – 1900)
Animal Feed:Research Diets D12492; Research Diets A14032002; Research Diets A14032001
Animal Inclusion Criteria:>45g bodyweight at 25 w/o

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id group Sample source
SA473590blank1blank blank
SA473576BAT-CF-6Control Brown adipose tissue
SA473577BAT-CF-5Control Brown adipose tissue
SA473578BAT-CF-7Control Brown adipose tissue
SA473579BAT-CF-8Control Brown adipose tissue
SA473580BAT-MR10-29MetR10 Brown adipose tissue
SA473581BAT-MR10-30MetR10 Brown adipose tissue
SA473582BAT-MR10-31MetR10 Brown adipose tissue
SA473583BAT-MR3-14MetR3 Brown adipose tissue
SA473584BAT-MR3-15MetR3 Brown adipose tissue
SA473585BAT-MR3-16MetR3 Brown adipose tissue
SA473586BAT-MR5-21MetR5 Brown adipose tissue
SA473587BAT-MR5-22MetR5 Brown adipose tissue
SA473588BAT-MR5-24MetR5 Brown adipose tissue
SA473589BAT-MR5-23MetR5 Brown adipose tissue
SA473591qc1qc pooled_qc
SA473592qc2qc pooled_qc
SA473593qc3qc pooled_qc
Showing results 1 to 18 of 18

Collection:

Collection ID:CO004222
Collection Summary:Brown adipose tissue samples were collected upon completion of the time course after fasting the mice for 4 h. A pooled QC sample was used for MS/MS collection.
Sample Type:Brown adipose
Storage Conditions:-80℃

Treatment:

Treatment ID:TR004238
Treatment Summary:Mice were weight-matched and divided into two groups fed either High fat diet, HFD-control (60% fat/0.86% methionine; Research Diets A14032002) or HFD-MetR (60% fat/0.12% methionine; Research Diets A14032001). The HFD-MetR group was split into 3 groups, where the HFD-MetR was administered for 3 days (MetR3, n=8), 5 days (MetR5, n=8), or 10 days (MetR10, n=7). HFD-MetR groups were compared to each other and the control group (HFD, n=8).
Treatment:Dietary intervention and duration of altered diet
Treatment Route:Diet
Treatment Dose:Research Diets A14032002; Research Diets A14032001
Treatment Dosevolume:HFD-control (60% fat/0.86% methionine; Research Diets A14032002) or HFD-MetR (60% fat/0.12% methionine; Research Diets A14032001)
Treatment Doseduration:3-10 days
Treatment Vehicle:Food
Animal Fasting:4h prior to sample collection
Animal Endp Tissue Coll List:Brown adipose tissue, liver, plasma

Sample Preparation:

Sampleprep ID:SP004235
Sampleprep Summary:Frozen tissue samples were homogenized with 80 μL 2:2:1 methanol:acetonitrile:water per mg tissue, centrifuged, and the supernatant was collected after a cold incubation and wash steps, then stored at -80°C. Plasma samples underwent solid-phase extraction (SPE) with sequential additions of acetonitrile:methanol and methanol:acetonitrile:water to separate polar and non-polar metabolites; polar metabolites were eluted and stored at -80°C, while non-polar metabolites were eluted using methyl tert-butyl ether:methanol, dried under nitrogen, reconstituted in isopropanol:methanol, and stored at -80°C.
Processing Storage Conditions:-80℃
Extract Storage:-80℃
Sample Resuspension:2:2:1 methanol:acetonitrile:water (polar), isopropanol:methanol (lipid)

Chromatography:

Chromatography ID:CH005138
Instrument Name:Agilent 1290 Infinity II
Column Name:HILICON iHILIC (P)-Classic (100 x 2.1mm, 5um)
Column Temperature:45
Flow Gradient:0-1 min: 90% B, 1-12 min: 90-35% B, 12-12.5 min: 35-20% B, 12.5 min-14.5 min: 20% B
Flow Rate:250 uL/min
Solvent A:95% water/5% acetonitrile; 20mM ammonium bicarbonate; 0.1% ammonium hydroxide; 2.5 µM medronic acid
Solvent B:95% water/5% acetonitrile
Chromatography Type:HILIC
  
Chromatography ID:CH005139
Instrument Name:Agilent 1290 Infinity II
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:45
Flow Gradient:0-2 min: 30% B, 2-17 min: 30-75% B, 17-20 min: 75-85% B, 20-23 min: 85-100% B, 23-26 min: 100% B, 26-27 min: 100-30% B
Flow Rate:400 uL/min
Solvent A:50% water/30% acetonitrile/20% isopropanol; 10 mM ammonium formate; 5 µM InfinityLab Deactivator Additive (Agilent)
Solvent B:90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid; 2.5 mM medronic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN006761
Analysis Type:MS
Chromatography ID:CH005138
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004083_AN006761_Results.txt
Units:log2-transformed normalized relative abundance
  
Analysis ID:AN006762
Analysis Type:MS
Chromatography ID:CH005138
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004083_AN006762_Results.txt
Units:log2-transformed normalized relative abundance
  
Analysis ID:AN006763
Analysis Type:MS
Chromatography ID:CH005139
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004083_AN006763_Results.txt
Units:log2-transformed normalized relative abundance
  
Analysis ID:AN006764
Analysis Type:MS
Chromatography ID:CH005139
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004083_AN006764_Results.txt
Units:log2-transformed normalized relative abundance
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