Summary of Study ST004178

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002635. The data can be accessed directly via it's Project DOI: 10.21228/M82253 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004178
Study TitleTomato-Soy Juice Reduces Inflammation and Modulates Urinary Metabolome in Adults with Obesity
Study SummaryScope: Chronic, low-grade inflammation is a hallmark of many noncommunicable diseases, including obesity. Diets enriched with tomatoes and soy have been associated with better health outcomes in inflammation-related illnesses, with lycopene and isoflavones considered key bioactive components, respectively. On the basis that whole food combinations may exert greater effects than isolated phytochemicals, we examine the anti-inflammatory and metabolic effects of tomato-soy juice compared to a low carotenoid tomato juice control in obesity. Methods and results: In a randomized, crossover trial, 12 healthy adults with obesity were provided either tomato-soy juice (54 mg lycopene/d, 189.9 mg isoflavones/d) or a low carotenoid tomato juice (no isoflavones) daily for 4 weeks, then crossed over to the other treatment following a washout period. Plasma carotenoids, cytokines, and the urine metabolome were measured pre- and post-interventions. Plasma lycopene significantly increased by 2.48-fold after tomato-soy intake. IL-5, IL-12p70, and GM-CSF significantly decreased (P < 0.05), and TNF-alpha trended downward (P = 0.052) following tomato-soy. Soy isoflavones and their metabolites primarily distinguished post-tomato-soy urine profiles. Both interventions induced some shared metabolomic changes in the urine, indicating tomato-driven effects independent of lycopene. Conclusion: Tomato-soy intake reduced some pro-inflammatory cytokines and altered the urine metabolomic profile in adults with obesity, supporting future studies using this functional food product for other inflammation-related conditions.
Institute
Ohio State University
Last NameCooperstone
First NameJessica
Address2255 Kenny Rd
Emailcooperstone.1@osu.edu
Phone614-292-2843
Submit Date2025-09-02
Num Groups2
Total Subjects12
Num Males7
Num Females5
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2025-09-30
Release Version1
Jessica Cooperstone Jessica Cooperstone
https://dx.doi.org/10.21228/M82253
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002635
Project DOI:doi: 10.21228/M82253
Project Title:Tomato-Soy Juice Reduces Inflammation and Modulates Urinary Metabolome in Adults with Obesity
Project Summary:Scope: Chronic, low-grade inflammation is a hallmark of many noncommunicable diseases, including obesity. Diets enriched with tomatoes and soy have been associated with better health outcomes in inflammation-related illnesses, with lycopene and isoflavones considered key bioactive components, respectively. On the basis that whole food combinations may exert greater effects than isolated phytochemicals, we examine the anti-inflammatory and metabolic effects of tomato-soy juice compared to a low carotenoid tomato juice control in obesity. Methods and results: In a randomized, crossover trial, 12 healthy adults with obesity were provided either tomato-soy juice (54 mg lycopene/d, 189.9 mg isoflavones/d) or a low carotenoid tomato juice (no isoflavones) daily for 4 weeks, then crossed over to the other treatment following a washout period. Plasma carotenoids, cytokines, and the urine metabolome were measured pre- and post-interventions. Plasma lycopene significantly increased by 2.48-fold after tomato-soy intake. IL-5, IL-12p70, and GM-CSF significantly decreased (P < 0.05), and TNF-alpha trended downward (P = 0.052) following tomato-soy. Soy isoflavones and their metabolites primarily distinguished post-tomato-soy urine profiles. Both interventions induced some shared metabolomic changes in the urine, indicating tomato-driven effects independent of lycopene. Conclusion: Tomato-soy intake reduced some pro-inflammatory cytokines and altered the urine metabolomic profile in adults with obesity, supporting future studies using this functional food product for other inflammation-related conditions.
Institute:Ohio State University
Last Name:Cooperstone
First Name:Jessica
Address:2255 Kenny Rd
Email:cooperstone.1@osu.edu
Phone:614-292-2843

Subject:

Subject ID:SU004329
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:30-60
Gender:Male and female
Human Smoking Status:Non-smokers
Human Inclusion Criteria:BMI 30-45, plasma total cholesterol levels at 250 mg/dL or lower, and triglyceride levels at 250 mg/dL or lower
Human Exclusion Criteria:Smoking, the use of daily prescription anti-inflammatory drugs, antibiotics within 3 months prior to the study, use of carotenoid/isoflavone/metabolism-altering supplements within 1 month of the study, presence of autoimmune or metabolic disorders, presence of any gastrointestinal or malabsorption conditions, indication of liver or kidney disorders, and tomato or soy allergies.

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Timepoint
SA4822986110_U2_C18NEG_34urine post Control
SA4822996113_U2_C18POS_31urine post Control
SA4823006112_U4_C18NEG_63urine post Control
SA4823016112_U4_C18POS_63urine post Control
SA4823026109_U2_HILICPOS_13urine post Control
SA4823036111_U2_C18NEG_35urine post Control
SA4823046111_U2_C18POS_35urine post Control
SA4823056110_U2_C18POS_34urine post Control
SA4823066111_U2_HILICPOS_35urine post Control
SA4823076109_U2_C18NEG_13urine post Control
SA4823086109_U2_C18POS_13urine post Control
SA4823096108_U4_C18NEG_18urine post Control
SA4823106108_U4_C18POS_18urine post Control
SA4823116112_U4_HILICNEG_63urine post Control
SA4823126112_U4_HILICPOS_63urine post Control
SA4823136106_U2_C18NEG_46urine post Control
SA4823146113_U2_C18NEG_31urine post Control
SA4823156111_U2_HILICNEG_35urine post Control
SA4823166105_U4_C18NEG_47urine post Control
SA4823176110_U2_HILICNEG_34urine post Control
SA4823186108_U4_HILICPOS_18urine post Control
SA4823196108_U4_HILICNEG_18urine post Control
SA4823206106_U2_HILICPOS_46urine post Control
SA4823216106_U2_HILICNEG_46urine post Control
SA4823226105_U4_HILICPOS_47urine post Control
SA4823236105_U4_HILICNEG_47urine post Control
SA4823246110_U2_HILICPOS_34urine post Control
SA4823256101_U4_HILICNEG_14urine post Control
SA4823266104_U2_HILICPOS_19urine post Control
SA4823276104_U2_HILICNEG_19urine post Control
SA4823286103_U4_HILICPOS_53urine post Control
SA4823296103_U3_HILICNEG_61urine post Control
SA4823306102_U4_HILICPOS_50urine post Control
SA4823316102_U3_HILICNEG_48urine post Control
SA4823326101_U4_HILICPOS_14urine post Control
SA4823336106_U2_C18POS_46urine post Control
SA4823346109_U2_HILICNEG_13urine post Control
SA4823356105_U4_C18POS_47urine post Control
SA4823366113_U2_HILICNEG_31urine post Control
SA4823376102_U4_C18NEG_50urine post Control
SA4823386113_U2_HILICPOS_31urine post Control
SA4823396104_U2_C18NEG_19urine post Control
SA4823406101_U4_C18NEG_14urine post Control
SA4823416101_U4_C18POS_14urine post Control
SA4823426103_U4_C18POS_53urine post Control
SA4823436104_U2_C18POS_19urine post Control
SA4823446103_U4_C18NEG_53urine post Control
SA4823456102_U4_C18POS_50urine post Control
SA4823466113_U4_C18POS_62urine post Tomato Soy
SA4823476113_U4_C18NEG_62urine post Tomato Soy
SA4823486101_U2_HILICNEG_30urine post Tomato Soy
SA4823496103_U2_C18POS_60urine post Tomato Soy
SA4823506101_U2_HILICPOS_30urine post Tomato Soy
SA4823516110_U4_HILICPOS_10urine post Tomato Soy
SA4823526103_U2_C18NEG_60urine post Tomato Soy
SA4823536110_U4_HILICNEG_10urine post Tomato Soy
SA4823546102_U1_HILICNEG_26urine post Tomato Soy
SA4823556102_U2_HILICPOS_16urine post Tomato Soy
SA4823566103_U2_HILICPOS_60urine post Tomato Soy
SA4823576103_U1_HILICNEG_21urine post Tomato Soy
SA4823586109_U4_HILICPOS_22urine post Tomato Soy
SA4823596101_U2_C18NEG_30urine post Tomato Soy
SA4823606101_U2_C18POS_30urine post Tomato Soy
SA4823616108_U2_HILICPOS_27urine post Tomato Soy
SA4823626108_U2_HILICNEG_27urine post Tomato Soy
SA4823636106_U4_HILICPOS_36urine post Tomato Soy
SA4823646106_U4_HILICNEG_36urine post Tomato Soy
SA4823656109_U4_HILICNEG_22urine post Tomato Soy
SA4823666105_U2_HILICPOS_40urine post Tomato Soy
SA4823676112_U2_C18POS_37urine post Tomato Soy
SA4823686105_U2_HILICNEG_40urine post Tomato Soy
SA4823696104_U4_HILICPOS_12urine post Tomato Soy
SA4823706104_U4_HILICNEG_12urine post Tomato Soy
SA4823716113_U4_HILICPOS_62urine post Tomato Soy
SA4823726113_U4_HILICNEG_62urine post Tomato Soy
SA4823736102_U2_C18POS_16urine post Tomato Soy
SA4823746102_U2_C18NEG_16urine post Tomato Soy
SA4823756112_U2_C18NEG_37urine post Tomato Soy
SA4823766111_U4_HILICNEG_42urine post Tomato Soy
SA4823776109_U4_C18NEG_22urine post Tomato Soy
SA4823786105_U2_C18POS_40urine post Tomato Soy
SA4823796108_U2_C18POS_27_1urine post Tomato Soy
SA4823806108_U2_C18NEG_27urine post Tomato Soy
SA4823816109_U4_C18POS_22urine post Tomato Soy
SA4823826112_U2_HILICNEG_37urine post Tomato Soy
SA4823836110_U4_C18POS_start-10urine post Tomato Soy
SA4823846106_U4_C18NEG_36urine post Tomato Soy
SA4823856106_U4_C18POS_36urine post Tomato Soy
SA4823866110_U4_C18NEG_10urine post Tomato Soy
SA4823876105_U2_C18NEG_40urine post Tomato Soy
SA4823886112_U2_HILICPOS_37urine post Tomato Soy
SA4823896104_U4_C18POS_12urine post Tomato Soy
SA4823906111_U4_HILICPOS_42urine post Tomato Soy
SA4823916104_U4_C18NEG_12urine post Tomato Soy
SA4823926111_U4_C18POS_42urine post Tomato Soy
SA4823936111_U4_C18NEG_42urine post Tomato Soy
SA4823946110_U1_HILICPOS_11urine pre Control
SA4823956105_U3_HILICNEG_39urine pre Control
SA4823966110_U1_HILICNEG_11urine pre Control
SA4823976108_U3_C18POS_28_1urine pre Control
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Collection:

Collection ID:CO004322
Collection Summary:At weeks 2, 6, 10, and 14, 24-hour urine samples were collected from the subjects into 4 L plastic jugs containing 10 g boric acid for preservation. Urine samples were then sub-sampled into smaller aliquots and stored at -80 degrees C.
Sample Type:Urine
Storage Conditions:-80℃

Treatment:

Treatment ID:TR004338
Treatment Summary:To test the effects of both lycopene and isoflavones on inflammation and the metabolome, subjects were instructed to consume diets with low levels of these phytochemicals for 2 weeks prior to the intervention. Counseling and a list of foods to avoid was provided to achieve a low lycopene/isoflavone diet. Following the 2-week washout period, subjects were randomized to receive 360 mL (two 180 mL cans) of either a soy-enriched, high lycopene tomato (tomato-soy) juice or a low carotenoid, yellow tomato juice (control) every day for 4 weeks. Followed by a 4-week washout period (low lycopene and isoflavone diet), participants began their second 4-week intervention consuming the other juice daily (Figure 2). At weeks 2, 6, 10, and 14, 24-hour urine samples were collected from the subjects into 4 L plastic jugs containing 10 g boric acid for preservation. Urine samples were then sub-sampled into smaller aliquots and stored at -80 degrees C. This study was approved by Chesapeake IRB (Pro00024511) and is registered on clinicaltrials.gov as NCT03783013.

Sample Preparation:

Sampleprep ID:SP004335
Sampleprep Summary:After thawing in cold water, urine was centrifuged for 10 min at 21,130 x g. Urine osmolality was measured by freezing point depression using an osmometer (Advanced Osmometer Model 3300; Norwood, MA, USA). To account for differences in urine volumes and hydration level between subjects/timepoints, each sample was normalized to 100 milliosmoles with water. Normalized urine samples were added to new sample tubes containing methanol and acetonitrile, so that the final mixture was the injection solvent 2:1:1 ACN/MeOH/H2O and centrifuged at 21,130 x g for 5 min to pellet any insoluble material. Process blanks were prepared using water following the same steps. Quality control (QC) samples were prepared by pooling 100 microliters from each normalized urine sample.
Processing Storage Conditions:Room temperature
Extract Storage:-80℃

Chromatography:

Chromatography ID:CH005265
Chromatography Summary:For each analysis, sample order was randomized, with QC samples in every 7th position (i.e., approximately every 1.5-2 h) to monitor instrument variability throughout the duration of the experimental run. Three process blanks were queued at the beginning of each run to account for residues and contamination from extraction materials and remove them from downstream analyses. For RPLC-MS analysis, 20 microliters was injected onto a 2.1 x 100 mm x 1.8 um Waters HSS column (Milford, MA, USA) kept at 40 degrees C.
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:held at 1% B for 0.5 min, increased linearly to 100% B over 10 minutes, held there for 1 min, then immediately decreased to 1% B and held there for 2 min for a total time of 13.5 min.
Flow Rate:0.4 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH005266
Chromatography Summary:For each analysis, sample order was randomized, with QC samples in every 7th position (i.e., approximately every 1.5-2 h) to monitor instrument variability throughout the duration of the experimental run. Three process blanks were queued at the beginning of each run to account for residues and contamination from extraction materials and remove them from downstream analyses. HILIC analysis was adapted from methods in Spagou et al (2011). Twenty uL was injected onto a 2.1 x 100 mm x 1.8 micron Waters Z-HILIC column maintained at 40 degrees C.
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Z-HILIC (100 x 2.1mm; 1.8um)
Column Temperature:40
Flow Gradient:The 14 min gradient was applied at 0.5 mL/min as described: held at 100% B for 1 min, increased linearly to 100% A over 10 min and held at this point for 1 min. Immediately afterwards, the gradient was held at 100% B for 2 min
Flow Rate:0.5 mL/min
Solvent A:50% acetonitrile/50% water; 10 mM ammonium formate (pH 7.2); 0.1% formic acid
Solvent B:95% acetonitrile/5% water; 10 mM ammonium formate (pH 7.2); 0.1% formic acid
Chromatography Type:HILIC

Analysis:

Analysis ID:AN006933
Analysis Type:MS
Chromatography ID:CH005265
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004178_AN006933_Results.txt
Units:Peak area
  
Analysis ID:AN006934
Analysis Type:MS
Chromatography ID:CH005265
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004178_AN006934_Results.txt
Units:Peak area
  
Analysis ID:AN006935
Analysis Type:MS
Chromatography ID:CH005266
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004178_AN006935_Results.txt
Units:Peak area
  
Analysis ID:AN006936
Analysis Type:MS
Chromatography ID:CH005266
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004178_AN006936_Results.txt
Units:Peak area
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