Summary of Study ST004242
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002675. The data can be accessed directly via it's Project DOI: 10.21228/M8W83V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004242 |
| Study Title | Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupus - lupus prone W.Yaa mouse treated with 2-deoxy-D-glucose |
| Study Summary | This study compares the cellular metabolomics of T follicular helper cells (Tfh) purified from the spleen of lupus-prone mice (W.Yaa) orally treated with 2DG versus untreated controls or non-autoimmune wild-type B6 controls. Age-matched W.Yaa male mice were treated with 2DG in drinking water (6 mg/ml) for 4 weeks or untreated for controls (CTRL). A group of age- and sex-matched C57BL/6 (B6) mice was used as non-autoimmune controls (B6 group). Murine Tfh cells were sorted from the mouse spleens as CD4+CD44+PD-1+PSGL-1lo cells. Metabolites were analyzed using LC-MS. The conclusion from this study is that 2DG treatment reprogrammed the metabolic profile of autoimmune Tfh cells toward that of non-autoimmune Tfh cells. |
| Institute | UT Health San Antonio |
| Department | Microbiology, Immunology. Molecular Genetics |
| Last Name | Yong |
| First Name | Ge |
| Address | 7703 Flyod Curl Dr |
| geyong725@gmail.com | |
| Phone | 210-567-3925 |
| Submit Date | 2025-09-25 |
| Num Groups | 3 |
| Total Subjects | 20 |
| Num Males | 20 |
| Publications | Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupus |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002675 |
| Project DOI: | doi: 10.21228/M8W83V |
| Project Title: | Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupus |
| Project Summary: | This study aimed to understand the impact of 2-deoxy-D-glucose (2DG) in modulating the transcriptional and metabolomic responses of T follicular helper cells (Tfh) in a lupus-prone mouse model (W.Yaa). Thus, we analyzed the metabolomes of Tfh cells purified the spleen of W.Yaa mice treated with 2DG versus untreated controls. Data demonstrated that inhibition of glycolysis via 2DG treatment profoundly reprogrammed the metabolic pathways of splenic Tfh cells. This includes the suppression of the pentose phosphate pathway, essential for autoimmune Tfh cell expansion and the activation of mitochondrial metabolism. The reprogrammed metabolism of Tfh cells was associated with the reduction of autoantibodies and autoimmune phenotype. |
| Institute: | UT Health San Antonio |
| Department: | Microbiology, Immunology. Molecular Genetics |
| Last Name: | Yong |
| First Name: | Ge |
| Address: | 7703 Flyod Curl Dr |
| Email: | geyong725@gmail.com |
| Phone: | 210-567-3925 |
| Funding Source: | NIH |
| Publications: | Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupus |
Subject:
| Subject ID: | SU004394 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Gender: | Male |
| Animal Light Cycle: | 12 hours interval |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Genotype | Treatment |
|---|---|---|---|---|
| SA490112 | B6-1 | Splenic Tfh cells | Wild-type | WT_Ctrl |
| SA490113 | B6-2 | Splenic Tfh cells | Wild-type | WT_Ctrl |
| SA490114 | B6-3 | Splenic Tfh cells | Wild-type | WT_Ctrl |
| SA490115 | B6-4 | Splenic Tfh cells | Wild-type | WT_Ctrl |
| SA490116 | B6-5 | Splenic Tfh cells | Wild-type | WT_Ctrl |
| SA490117 | B6-6 | Splenic Tfh cells | Wild-type | WT_Ctrl |
| SA490098 | 2DG-6 | Splenic Tfh cells | W.Yaa | 2DG |
| SA490099 | 2DG-2 | Splenic Tfh cells | W.Yaa | 2DG |
| SA490100 | 2DG-7 | Splenic Tfh cells | W.Yaa | 2DG |
| SA490101 | 2DG-1 | Splenic Tfh cells | W.Yaa | 2DG |
| SA490102 | 2DG-5 | Splenic Tfh cells | W.Yaa | 2DG |
| SA490103 | 2DG-4 | Splenic Tfh cells | W.Yaa | 2DG |
| SA490104 | 2DG-3 | Splenic Tfh cells | W.Yaa | 2DG |
| SA490105 | CTRL-1 | Splenic Tfh cells | W.Yaa | Ctrl |
| SA490106 | CTRL-2 | Splenic Tfh cells | W.Yaa | Ctrl |
| SA490107 | CTRL-3 | Splenic Tfh cells | W.Yaa | Ctrl |
| SA490108 | CTRL-5 | Splenic Tfh cells | W.Yaa | Ctrl |
| SA490109 | CTRL-6 | Splenic Tfh cells | W.Yaa | Ctrl |
| SA490110 | CTRL-7 | Splenic Tfh cells | W.Yaa | Ctrl |
| SA490111 | CTRL-4 | Splenic Tfh cells | W.Yaa | Ctrl |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO004387 |
| Collection Summary: | Splenic Tfh cells were purified by flow cytometry. W.Yaa mice were treated with 2DG in drinking water or untreated (CTRL). B6 mice were used for non-autoimmune controls. Thus, 3 groups of mice (2DG, CTRL, and B6) and 2 genotypes (W.yaa and Wild-type) were specified. W.Yaa mice were bred in our mouse colonies at the Animal Facility of UT Health San Antonio |
| Sample Type: | Splenic T follicular helper cells |
| Collection Method: | Flow cytometry |
Treatment:
| Treatment ID: | TR004403 |
| Treatment Summary: | Age-matched W.Yaa mice were orally treated with 2DG (6 mg/ml) in drinking water or untreated (Ctrl). Mice were euthanized after 4 weeks of treatment with 2DG to isolate Tfh cells for metabolite analyses. A group of non-autoimmune B6 control mice was included. |
Sample Preparation:
| Sampleprep ID: | SP004400 |
| Sampleprep Summary: | Global metabolomics profiling was performed on a Thermo Q-Exactive Oribtrap mass spectrometer with Dionex UHPLC and autosampler. Samples were homogenized in 5 mM ammonium acetate at 20 mg/ml. After homogenization, the sample was centrifuged at 20,000 rcf and 100 l was transferred to a new tube. Next, 800 μL of a mixture of acetonitrile, methanol, and acetone (8:1:1) was added followed by centrifugation to precipitate and pellet the proteins. The supernatant was transferred to a clean tube and dried under a gentle stream of nitrogen before reconstitution in 100 μL of 0.1% formic acid in water for metabolomic analysis. |
Chromatography:
| Chromatography ID: | CH005362 |
| Chromatography Summary: | Separation was achieved on an ACE C18-pfp 100 x 2.1 mm, 2 µm column with mobile phase A as 0.1% formic acid in water and mobile phase B as acetonitrile. The flow rate was 350 µl/min with a column temperature of 25°C. 4 µl were injected for negative ions and 2 µL for positive ions. |
| Instrument Name: | Thermo Dionex |
| Column Name: | ACE C18-PFP (100 x 2.1 mm, 2 um) |
| Column Temperature: | 25°C |
| Flow Gradient: | No flow gradient |
| Flow Rate: | 350 µL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN007061 |
| Analysis Type: | MS |
| Chromatography ID: | CH005362 |
| Num Factors: | 3 |
| Num Metabolites: | 142 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST004242_AN007061_Results.txt |
| Units: | TIC-normalized intensity |
| Analysis ID: | AN007062 |
| Analysis Type: | MS |
| Chromatography ID: | CH005362 |
| Num Factors: | 3 |
| Num Metabolites: | 135 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST004242_AN007062_Results.txt |
| Units: | TIC-normalized intensity |
