Summary of Study ST004259
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002687. The data can be accessed directly via it's Project DOI: 10.21228/M8B85N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004259 |
| Study Title | Phytate enhances gut Parasutterella colonization to alleviate radiation injury via anti-inflammatory and antioxidant effects |
| Study Summary | Food as medicine shows promise for disease intervention or treatment. Here, we found phytate, an active ingredient of plant-based diets, exhibits properties in mitigating radiotherapy-related complications. Oral gavage of phytate restored hematogenic organ atrophy, elevated peripheral blood neutrophils and white blood cells, reduced inflammation, and improved gastrointestinal (GI) integrity in irradiated mice. Phytate intake modulated the gut microbiota, facilitating the colonization of symbiotic Parasutterella in GI tract, thus combating intestinal radiation toxicity. In vitro assays and untargeted metabolomics identified 3-Phenyllactic acid (PLA) and N-Acetyl-L-leucine (NL) as functional metabolites produced by Parasutterella. In vitro, ex vivo, and in vivo models showed that PLA induces M2-like polarization in macrophages, while NL reduced oxidative stress, both counteracting radiation toxicity and working synergistically. Our findings offer mechanistic insights into phytate for alleviating radiation-associated complications and suggest that Parasutterella and its metabolites might be employed as promising probiotics or postbiotics for cancer patients with radiotherapy. |
| Institute | Chinese Academy of Medical Sciences |
| Last Name | Li |
| First Name | Yuan |
| Address | Baidi Road, Tianjin, Tianjin, 300192, China |
| liyuan@irm-cams.ac.cn | |
| Phone | +8615900383739 |
| Submit Date | 2025-09-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002687 |
| Project DOI: | doi: 10.21228/M8B85N |
| Project Title: | Phytate enhances gut Parasutterella colonization to alleviate radiation injury via anti-inflammatory and antioxidant effects |
| Project Summary: | Food as medicine shows promise for disease intervention or treatment. Here, we found phytate, an active ingredient of plant-based diets, exhibits properties in mitigating radiotherapy-related complications. Oral gavage of phytate restored hematogenic organ atrophy, elevated peripheral blood neutrophils and white blood cells, reduced inflammation, and improved gastrointestinal (GI) integrity in irradiated mice. Phytate intake modulated the gut microbiota, facilitating the colonization of symbiotic Parasutterella in GI tract, thus combating intestinal radiation toxicity. In vitro assays and untargeted metabolomics identified 3-Phenyllactic acid (PLA) and N-Acetyl-L-leucine (NL) as functional metabolites produced by Parasutterella. In vitro, ex vivo, and in vivo models showed that PLA induces M2-like polarization in macrophages, while NL reduced oxidative stress, both counteracting radiation toxicity and working synergistically. Our findings offer mechanistic insights into phytate for alleviating radiation-associated complications and suggest that Parasutterella and its metabolites might be employed as promising probiotics or postbiotics for cancer patients with radiotherapy. |
| Institute: | Chinese Academy of Medical Sciences |
| Last Name: | Li |
| First Name: | Yuan |
| Address: | Baidi Road, Tianjin, Tianjin, 300192, China |
| Email: | liyuan@irm-cams.ac.cn |
| Phone: | +8615900383739 |
Subject:
| Subject ID: | SU004412 |
| Subject Type: | Bacteria |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
Subject type: Bacteria; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment | Sample source |
|---|---|---|---|
| SA496928 | PE0h-6 | Sample collection after 0h of cultivation | Culture supernatant |
| SA496929 | PE0h-2 | Sample collection after 0h of cultivation | Culture supernatant |
| SA496930 | PE0h-1 | Sample collection after 0h of cultivation | Culture supernatant |
| SA496931 | PE0h-5 | Sample collection after 0h of cultivation | Culture supernatant |
| SA496932 | PE0h-4 | Sample collection after 0h of cultivation | Culture supernatant |
| SA496933 | PE0h-3 | Sample collection after 0h of cultivation | Culture supernatant |
| SA496934 | PE24h-1 | Sample collection after 24h of cultivation | Culture supernatant |
| SA496935 | PE24h-2 | Sample collection after 24h of cultivation | Culture supernatant |
| SA496936 | PE24h-3 | Sample collection after 24h of cultivation | Culture supernatant |
| SA496937 | PE24h-4 | Sample collection after 24h of cultivation | Culture supernatant |
| SA496938 | PE24h-5 | Sample collection after 24h of cultivation | Culture supernatant |
| SA496939 | PE24h-6 | Sample collection after 24h of cultivation | Culture supernatant |
| SA496940 | PE6h-1 | Sample collection after 6h of cultivation | Culture supernatant |
| SA496941 | PE6h-2 | Sample collection after 6h of cultivation | Culture supernatant |
| SA496942 | PE6h-3 | Sample collection after 6h of cultivation | Culture supernatant |
| SA496943 | PE6h-5 | Sample collection after 6h of cultivation | Culture supernatant |
| SA496944 | PE6h-6 | Sample collection after 6h of cultivation | Culture supernatant |
| SA496945 | PE6h-4 | Sample collection after 6h of cultivation | Culture supernatant |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO004405 |
| Collection Summary: | Culture supernatants of Parasutterella excrementihominis (DSM 21040) were collected at 0, 6, and 24 hours, followed by centrifugation at 3000 rpm for 10 minutes at 4ºC. The samples were then rapidly frozen in liquid nitrogen for 15 minutes and stored at -80ºC until further use. |
| Sample Type: | Media |
Treatment:
| Treatment ID: | TR004421 |
| Treatment Summary: | Not applicable |
Sample Preparation:
| Sampleprep ID: | SP004418 |
| Sampleprep Summary: | The samples (1 mL) were freeze-dried and resuspended with prechilled 80% methanol by well vortex. Then the samples were incubated on ice for 5 min and centrifuged at 15,000 g, 4°C for 15 min. Some of supernatant was diluted to final concentration containing 53% methanol by LC-MS grade water. The samples were subsequently transferred to a fresh Eppendorf tube and were centrifuged at 15000 g, 4°C for 15 min. Finally, the supernatant was injected into the LC-MS/MS system analysis. |
Chromatography:
| Chromatography ID: | CH005384 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Hypersil GOLD aQ (100 x 2.1 mm, 1.9 μm) |
| Column Temperature: | 40°C |
| Flow Gradient: | 2%B, 1.5 min;2-85% B, 3 min; 85-100% B, 10 min；100-2% B, 10.1 min；2%B, 12 min. |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 100% Water; 0.1% Formic acid |
| Solvent B: | 100% Methanol |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH005385 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Hypersil GOLD aQ (100 x 2.1 mm,1.9 μm) |
| Column Temperature: | 40°C |
| Flow Gradient: | 2%B, 1.5 min;2-85% B, 3 min; 85-100% B, 10 min；100-2% B, 10.1 min；2%B, 12 min. |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 100% Water; 5 mM Ammonium acetate, pH9.0 |
| Solvent B: | 100% Methanol |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN007087 |
| Analysis Type: | MS |
| Chromatography ID: | CH005384 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST004259_AN007087_Results.txt |
| Units: | Peak area |
| Analysis ID: | AN007088 |
| Analysis Type: | MS |
| Chromatography ID: | CH005385 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST004259_AN007088_Results.txt |
| Units: | Peak area |
