Summary of Study ST004264

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002691. The data can be accessed directly via it's Project DOI: 10.21228/M8T837 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004264
Study TitleDynamic metabolic and molecular changes during seasonal shrinking in Sorex araneus
Study TypeMS quantitative analysis
Study SummaryTo meet the challenge of wintering in place many high-latitude small mammals reduce energy demands through hibernation. In contrast, short-lived Eurasian common shrews, Sorex araneus, remain active and shrink, including energy-intensive organs in winter, regrowing in spring in an evolved strategy called Dehnel’s phenomenon. How this size change is linked to metabolic and regulatory changes to sustain their high metabolism is unknown. We analyzed metabolic, proteomic, and gene expression profiles spanning the entirety of Dehnel’s seasonal cycle in wild shrews. We show regulatory changes to oxidative phosphorylation and increased fatty acid metabolism during autumn-to-winter shrinkage, as previously found in hibernating species. But in shrews we also found upregulated winter expression of genes involved in gluconeogenesis: the biosynthesis of glucose from non-carbohydrate substrates. Co-expression models revealed changes in size and metabolic gene expression interconnect via FOXO signaling, whose overexpression reduces size and extends lifespan in many model organisms. We propose that while shifts in gluconeogenesis meet the challenge posed by high metabolic rate and active winter lifestyle, FOXO signaling is central to Dehnel’s phenomenon, with spring downregulation limiting lifespan in these shrews.
Institute
Aalborg University
DepartmentHealth Science and Technology
LaboratoryMolecular Pharmacology
Last NameZeng
First NameYuanyuan
AddressThulevej 34 Aalborg SØ, Aalborg, Aalborg, 9210, Denmark
Emailyuanyzeng@outlook.com
Phone+4555204123
Submit Date2025-09-16
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-11-03
Release Version1
Yuanyuan Zeng Yuanyuan Zeng
https://dx.doi.org/10.21228/M8T837
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002691
Project DOI:doi: 10.21228/M8T837
Project Title:Dynamic metabolic and molecular changes during seasonal shrinking in Sorex araneus
Project Type:MS quantitative analysis
Project Summary:Eurasian common shrews (Sorex araneus) maintain exceptionally high metabolic rates year-round, posing a significant energetic challenge during winter when food availability declines. To investigate seasonal metabolic regulation, we performed metabolomic profiling of blood—central to energy homeostasis—across seasons, using a bottom-up/shotgun DDA LC-MSMS metabolomics analytical strategy. This approach reveals molecular adaptations that support metabolic resilience and highlights key regulatory shifts in response to environmental stress.
Institute:Aalborg University
Department:Health Science and Technology
Laboratory:Molecular Pharmacology
Last Name:Zeng
First Name:Yuanyuan
Address:Thulevej 34 Aalborg SØ, Aalborg, Aalborg, 9210, Denmark
Email:yuanyzeng@outlook.com
Phone:55204123

Subject:

Subject ID:SU004417
Subject Type:Mammal
Subject Species:Sorex araneus
Taxonomy ID:42254
Gender:Not applicable
Animal Animal Supplier:Max Plank Institute of Animal Behavior
Animal Inclusion Criteria:wild types across different seasons

Factors:

Subject type: Mammal; Subject species: Sorex araneus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Group
SA497281FALLJUV20_1Blood FallJuveniles
SA497282FALLJUV20_2Blood FallJuveniles
SA497283FALLJUV20_3Blood FallJuveniles
SA497284FALLJUV20_4Blood FallJuveniles
SA497285SPRINGAD21_2Blood SpringAdults
SA497286SPRINGAD21_5Blood SpringAdults
SA497287SPRINGAD21_4Blood SpringAdults
SA497288SPRINGAD21_3Blood SpringAdults
SA497289SPRINGAD21_1Blood SpringAdults
SA497290SUMAD21_5Blood SummerAdults
SA497291SUMAD21_4Blood SummerAdults
SA497292SUMAD21_3Blood SummerAdults
SA497293SUMAD21_2Blood SummerAdults
SA497294SUMAD21_1Blood SummerAdults
SA497295SUMJUV20_1Blood SummerJuveniles
SA497296SUMJUV20_2Blood SummerJuveniles
SA497297SUMJUV20_5Blood SummerJuveniles
SA497298SUMJUV20_4Blood SummerJuveniles
SA497299SUMJUV20_3Blood SummerJuveniles
SA497300WINTERJUV21_5Blood WinterJuveniles
SA497301WINTERJUV21_3Blood WinterJuveniles
SA497302WINTERJUV21_2Blood WinterJuveniles
SA497303WINTERJUV21_1Blood WinterJuveniles
SA497304WINTERJUV21_4Blood WinterJuveniles
Showing results 1 to 24 of 24

Collection:

Collection ID:CO004410
Collection Summary:Eurasian common shrews were trapped using insulated wooden traps in Radolfzell, Germany, at five different stages of development: large-brained summer juveniles (n=5), shrinking autumn juveniles (n=4), small winter juveniles (n=5), regrowing spring adults (n=5), and regrown summer adults (n=5). Blood was collected prior to perfusion through the heart, let stand for 15 minutes, spun down at 1200 rpm, the serum pipetted off and then stored at −80°C. Shrews were perfused under anesthesia via the vascular system with PAXgene Tissue Fixative.
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR004426
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP004423
Sampleprep Summary:Plasma was aliquoted and stored at −80 °C until extraction. For metabolite extraction, frozen aliquots were thawed on ice and proteins were precipitated by adding ice-cold organic solvent (methanol), followed by vortex mixing, brief incubation on ice, and centrifugation at high speed (4 °C). The clarified supernatant was transferred to new tubes and reconstituted in the initial LC–MS solvent prior to analysis. Blank and pooled QC extracts were prepared and processed alongside study samples under the same conditions.
Extract Storage:-80℃

Chromatography:

Chromatography ID:CH005390
Chromatography Summary:The analysis was carried out using a UPLC system (Vanquish, Thermo Fisher Scientific) coupled with a high-resolution quadrupole-orbitrap mass spectrometer (Q Exactive™ HF Hybrid Quadrupole-Orbitrap, Thermo Fisher Scientific). An electrospray ionization interface was used as ionization source. Analysis was performed in negative and positive ionization mode. A QC sample was analysed in MS/MS mode for identification of compounds. The UPLC was performed using a slightly modified version of the protocol described by Catalin et al. (UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes, Water Application note 2011, 720004042en).
Methods Filename:Waters_Water_Soluble_Vitamin-Bs.pdf
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
Column Temperature:30 °C
Flow Gradient:0.0 min 0% B 2.0 min 0% B 12.0 min 35% B 13.0 min 90% B 14.0 min 90% B 15.0 min 0% B
Flow Rate:300 μL/min
Solvent A:100% water; 10 mM ammonium formate; 0.1% formic acid (pH 3.1)
Solvent B:100% methanol; 10 mM ammonium formate, 0.1% formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN007096
Analysis Type:MS
Chromatography ID:CH005390
Num Factors:5
Num Metabolites:170
Units:Peak area
  
Analysis ID:AN007097
Analysis Type:MS
Chromatography ID:CH005390
Num Factors:5
Num Metabolites:72
Units:Peak area
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